Labshake search
Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 6- to 12-week-old mice were orally administrated with 180mg/kg of Tamoxifen (TAM) (Sigma-Aldrich, St. Louis, MO, T5648-5G) for 5 days ...
-
bioRxiv - Immunology 2023Quote: ... Plates were then blocked for 6 h with PBST (PBS with 0.01% Tween-20) containing 2% BSA (Sigma-Aldrich; cat. no. 10735094001) at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... double-stained cells were also stained with 10 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine, Sigma Aldrich, Zwijndrecht, the Netherland). Laurdan was excited at 395 nm and 470 nm emission wavelength was used for rigid membrane and 508 nm for fluid membrane environment ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells were incubated at 37 °C and 5% CO2 for 24 hrs and selected with growth medium containing 6 μg/mL puromycin (Sigma-Aldrich, P8833) and 750 μg/mL neomycin (Thermo ...
-
bioRxiv - Cell Biology 2023Quote: ... permeabilized for 4-6 h with 10% goat serum in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and incubated overnight at 4°C in primary antibody in PBS containing 0.1% Tween 20 (PBS-Tw ...
-
bioRxiv - Cell Biology 2023Quote: Recombination of the conditional tm1c allele was induced in utero by injecting time pregnant females with a single intraperitoneal (IP) injection of 6 mg tamoxifen (Millipore Sigma, T5648)/40 g body weight dissolved in corn oil ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
-
bioRxiv - Microbiology 2024Quote: ... Pre-seeded A549 cells were subsequently transduced with the lentivirus in the presence of 6 μg/ml of polybrene (Sigma-Aldrich, TR1003). After 24 hours ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
-
bioRxiv - Immunology 2024Quote: ... IL-6 and IL-17A were assessed using the High Sensitivity T-cell Discovery Array 3-Plex (Millipore, St. Charles, MO, USA) at Eve Technologies using the Bio-Plex™ 200 system (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were permeabilised overnight at 4°C and then transferred to blocking solution (10% DMSO, 6% normal donkey serum (NDS; Sigma-Aldrich, D9663), 0.2% Triton X-100 in DPBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 5 µg/ml blasticidin S and surviving colonies were isolated using 6 mm Pyrex® cloning cylinder (Sigma-Aldrich). Gene knockout was verified by western blotting of whole cell lysates with appropriate antibodies.
-
bioRxiv - Genetics 2021Quote: We mixed on ice 500 µl of microspore suspension in TriReagent with 300 µl glass beads (Sigma, G8772-100G glass beads acid washed) and two sterilized metals beads (3mm diameter) ...
-
bioRxiv - Microbiology 2021Quote: ... Blood-fed mosquitoes were then aspirated into a new cup then provided with 10% sucrose supplemented with 0.05% para-aminobenzoic acid (PABA, Sigma, Cat no. A9878, USA) and maintained at 19±1°C ...
-
bioRxiv - Biochemistry 2020Quote: ... each cage was fed on defibrinated horse blood spiked with distilled water (control) or a serial dilution of DL-p-hydroxyphenyllactic acid (HPLA: Aldrich-Sigma cat no. H3253). All dilutions were made with the same ratio ...
-
bioRxiv - Biochemistry 2021Quote: ... rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM; Sigma-Aldrich, St. Louis, MO), Atto 465-p (8 μM) ...
-
bioRxiv - Plant Biology 2021Quote: ... culture was harvested and washed in 50 ml of buffer A [10 mM 2-ethanesulfonic acid (MES; Sigma-Aldrich, St. Louis, MO, USA) at pH 5.6 and 10 mM MgCl2] before resuspended to a final OD600 = 1.0 with buffer A with 200 µM acetosyringone (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: LC-MS grade water was purchased from Honeywell (Riedel-de Hae n; LOT: I174P) and formic acid was purchased from Sigma-Aldrich (LOT: 179247). LiChromasolv hypergrade Acetonitrile (LOT ...
-
bioRxiv - Microbiology 2020Quote: ... Each strain was grown overnight in GMM (20 mM glucose, 5 mL culture tubes) supplemented with 1x MEM amino acids (AA) (Sigma, Cat. No. M5550). Cells were pelleted ...
-
bioRxiv - Cancer Biology 2020Quote: ... diluted to a concentration of 200 μM per amino acid was added to 600 μL 80% HPLC grade methanol (Sigma-Aldrich, 646377-4X4L) and added to cells after draining media and washing with 2-3 mL ice cold saline ...
-
bioRxiv - Cancer Biology 2021Quote: ... Serial sections for trabecular bone were obtained from the distal femur at a thickness of 5 µm followed by tartrate-resistant acid phosphatase (TRAP) staining or alkaline phosphatase (ALP) staining using TRAP kits (Fast Red TR/Naphthol AS-MX, Sigma, St. Louis, MO) and NBT/BCIP (Thermo Scientific) ...
-
bioRxiv - Plant Biology 2022Quote: ... normalizing protein levels to the Rubisco large subunit visualized in membranes stained with Ponceau (0.1 % Ponceau S Sigma-Aldrich in 5 % acetic acid). The statistical differences between the average protein levels of each sample were inferred using a Student’s t-test.
-
bioRxiv - Bioengineering 2022Quote: ... A 400-μL cell culture medium was clarified by addition of trichloroacetic acid (200-μL, 30% by weight; Sigma Aldrich, St. Louis, MO), followed by vortex mixing and centrifugation at 8,000g for 5 min ...
-
bioRxiv - Physiology 2021Quote: ... followed by Sirius red staining consisting of a 30-minute incubation in 0.1% Sirius red F3B (1A 280; Chroma Gesellschaft, Germany) in saturated picric acid (Sigma-Aldrich, St Louis, MO), and several incubations with ethyl alcohol and xylene ...
-
bioRxiv - Neuroscience 2020Quote: ... The cochlea was post-fixed for a further 2 hours prior to rinsing with phosphate buffered saline (PBS) and decalcification in 0.12 M ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich, St Louis, MO) for 48 hours on an orbital shaker at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained using 0.1% Picrosirius red (Direct Red 80, Sigma-Aldrich, Cat# 365548 and picric acid solution, Sigma-Aldrich, Cat# P6744) and counterstained with Weigert’s hematoxylin (Cancer Diagnostics ...
-
bioRxiv - Microbiology 2022Quote: ... Durham, NC) or Brucella agar (Becton Dickinson, Canaan, CT) supplemented with 500uL of PBS with 0.1% taurocholic acid (Millipore-Sigma, St. Louis, MO) for C ...
-
bioRxiv - Cancer Biology 2022Quote: ... To visualize cellular morphologies and protein distribution for live cells grown in culture, cells were grown on acid-washed coverslips (Fisher, Cat. no. NC0706236) coated with bovine gelatin (Sigma, Cat. no. G1393), fixed with 4% paraformaldehyde and stained with Alexa Fluor 594-labelled phalloidin (ThermoFisher ...
-
bioRxiv - Microbiology 2019Quote: ... Capsule quantitation was assayed by measuring uronic acid content on ethanol-precipitated culture supernatants by addition of 1.2 mL of 12.5 mM tetraborate (Sigma-Aldrich, St. Louis, MO, USA) in concentrated sulphuric acid (Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and formic acid (FA) were purchased from Biosolve (Valkenswaard, The Netherlands) and water was purified using a Milli-Q plus system (Millipore, Amsterdam, The Netherlands). Bovine plasma was obtained from Biowest (Amsterdam ...
-
bioRxiv - Systems Biology 2020Quote: ... Supernatants were subsequently filtered using a 0.2 µm filter and purified by nickel affinity chromatography with nickel-activated nitrilotriacetic acid-agarose (Ni-NTA) (Novagen, San Diego, CA, USA). His-tagged AltD and AtlF proteins eluted with 20 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... Both 1:1 and 1:10 dilutions of each sample were prepared in duplicate with 0.5X SYBR Green I nucleic acid gel stain (Sigma-Aldrich, St. Louis, MO), plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) used for cell differentiation were purchased from Sigma-Aldrich (R2625 and P8139, respectively). According to our previous work (Carmona et al. ...
-
bioRxiv - Microbiology 2019Quote: ... Both 1:1 and 1:10 dilutions of each sample were prepared in duplicate with 0.5X SYBR Green I nucleic acid gel stain (Sigma-Aldrich, St. Louis, MO), plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs ...
-
bioRxiv - Cell Biology 2021Quote: ... After alkylation, 5 µg of mass spectrometry (MS) grade trypsin (Fisher, PI90057) dissolved in 5 µL 50 mM acetic acid (Sigma, 45754-100ML-F) was added to proteins on beads ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
-
bioRxiv - Microbiology 2020Quote: ... 1ml of the medium/well) were incubated at 37°C for 24 hours with 0.1 nM retinoic acid (Sigma-Aldrich, St. Louis, MO) and 2 ng/ml recombinant mouse TGF-β (R&D Systems ...
-
bioRxiv - Immunology 2021Quote: ... a mixture of chicken type II collagen dissolved in 0.1M acetic acid was mixed with an equal volume of complete Freund’s adjuvant (all reagents purchased from Sigma-Aldrich, Saint Louis, MO, USA). Eight week-old mice were then anesthetized using isoflurane (2% ...
-
bioRxiv - Immunology 2020Quote: ... combined with acetic acid (0.1%, pH 4.2, Biosolve, Valkenswaard, Netherlands) in water or acetonitrile (LC-MS CHROMASOLV, FLUKA, Sigma Aldrich, St Louis, MO, USA). Mass spectrometry analysis was performed in negative electrospray ionization mode ...
-
bioRxiv - Bioengineering 2020Quote: We then prepared and autoclaved a 2% w/v alginate (Pronova UltraPure MVG, NovaMatrix, Drammen, Norway) or 7.5% w/v alginate (Alginic acid sodium salt, Millipore Sigma, St Louis, MO) in HEPES saline buffer solution (Ultrasaline A ...
-
bioRxiv - Developmental Biology 2022Quote: Samples were isolated from whole brain as described above and proteins were precipitated from lysis buffer using Trichloroacetic acid (TCA) as follows: 50μl of 100% TCA (Sigma/Millipore – cat# T6399-5G) was added to 200 μl of protein sample at a final concentration of 20% ...
-
bioRxiv - Biochemistry 2022Quote: ... a RRP17-HA-AID strain was transformed with plasmids carrying respective constructs and growth assessed upon Rrp17-HA-AID depletion on plates containing 2 mM auxin (3-indoleacetic acid) (Sigma-Aldrich, Cat# I2886). Genetic experiments were performed at least in two biological replicates.
-
bioRxiv - Biochemistry 2022Quote: ... Cells were inoculated into 200 ml MGY liquid media composed of 1.34% yeast nitrogen base without amino acids (Millipore Sigma, St. Louis, MO), 0.4 mg/L biotin ...
-
bioRxiv - Cell Biology 2022Quote: ... Strains containing AID tag alleles were treated with final concentrations of 250 µM 3-indole acetic acid (Sigma-Aldrich, St. Louis, MO, USA) in DMSO and buffered with 50 mM potassium phosphate buffer at pH 6.2 for the 30 minutes prior to harvesting or imaging ...
-
bioRxiv - Cancer Biology 2022Quote: ... T1426), urea (Cat. 5378), dithiothreitol (Cat. 43816), Iodoacetamide (Cat. A3221) and trifluoroacetic acid (TFA, Cat. 302031) were obtained from Sigma-Aldrich (Darmstadt, Germany). Tris-HCl (Cat ...
-
bioRxiv - Developmental Biology 2022Quote: ... Washing steps were performed using PBS supplemented with 0,5 % w/v bovine serum albumin and 0,1 % sodium acid (both Sigma-Aldrich, St. Louis, MO, USA). Surface marker incubation was performed before intracellular staining at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... LO2 cells and AML12 cells were treated with cell culture medium containing the indicated concentrations of palmitic acid (PA, P9767; Sigma-Aldrich, MO, USA) and oleic acid (OA ...
-
bioRxiv - Molecular Biology 2022Quote: A 558 bp region of the brm CDS (Flybase: FBpp0075278) encoding amino acids 1321-1506 was cloned into pET28a-6XHis (Sigma, St. Louis, MO). The construct was transformed into BL21-DE3 E ...
-
bioRxiv - Plant Biology 2023Quote: ... ABA treatments were initiated by transferring 8-day-old seedlings in mass to plates containing 50 µM ABA (Sigma cis/trans-abscisic acid) or to plates containing vehicle control (Ethanol) ...