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Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... we used a solution containing 25 mg/mL yeast extract and 4 mg/mL nalidixic acid in artificial sea water (Sigma-Aldrich, MO, USA) (13) ...
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bioRxiv - Physiology 2023Quote: The cytochrome c oxidase (COX) activity was measured in the presence of Ascorbate-Na (2mM; Sigma-Aldrich, A4034; pH6 with Ascorbic acid (Sigma-Aldrich, A7506)) and N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in osteogenic medium (α-MEM-complete, supplemented with 2 mM β- glycerophosphate (BGP) and 50 µg/ml L-ascorbic acid (both Sigma-Aldrich)) ...
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bioRxiv - Systems Biology 2023Quote: ... 5 g/L yeast extract), supplemented with antibiotics (kanamycin, 50 μg/mL and spectinomycin, 150 μg/mL) or 10 mM Diaminopimelic acid (Sigma Aldrich, cat# D1377) when required ...
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bioRxiv - Biochemistry 2023Quote: ... protein extracts from the insoluble fraction were diluted in blue native sample buffer (25 mM BisTris, Bio-Rad; 0.015 1N Hydrochloric Acid, Fisher Scientific; 10% glycerol, Sigma Aldrich; 25 mM NaCl, Sigma Aldrich; 0.001% Ponceau S ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Sigma cat. C-0759; 0.0874 M sodium citrate, Sigma, cat. S-4641; pH 6.0) for a total of 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was transferred to clean glass tubes and D27-myristic acid (0.15 µg/µL, Cat No 366889, Sigma-Aldrich; St Louis, MO) was added as internal run standard ...
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bioRxiv - Biochemistry 2024Quote: ... Acetonitrile (CHROMASOLV® Plus for HPLC, ≧99.9%), formic acid (ACS reagent ≧96%), and acetone (CHROMASOLV® Plus for HPLC, ≧99.9%) were obtained from Sigma-Aldrich (St. Louis, MO). Triton X-100 was obtained from ICN Biochemicals Inc ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were added at 5,000 cells/cm2 to 150 mm Petri dishes and media supplemented with a final concentration of 50 µM ascorbic acid 2-phosphate (Sigma Aldrich, Cat. # A8960). Media was exchanged every 3 days and cells were cultured for 2.5 weeks ...
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bioRxiv - Bioengineering 2024Quote: ... tuberculosis H37Rv was grown in 7H9 broth supplemented with 10% oleic acid, albumin, dextrose, catalase ([OADC] Difco Laboratories, Detroit, MI) and 0.05% Tween 80 (Sigma-Aldrich, St. Louis, MO) before infection ...
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bioRxiv - Neuroscience 2024Quote: ... mice were intra-amygdalally (i.a.) injected (ventral [V], −2.0 mm below the guide cannula) with either kainic acid (i.a.KA, Sigma-Aldrich, USA; 0.3 µg/2µL; n=30) to induce status epilepticus or sterile phosphate-buffered saline (i.a.PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 24.75 µl of activity assay buffer (0.25% w/v taurocholic acid in citrate/phosphate buffer) containing 2 mM 4-methylumbelliferyl-β-D-glucopyranoside substrate (4MU-Gluc; Sigma-Aldrich, # M3633) in activity assay buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The synthetic auxin α-Naphthaleneacetic acid (NAA) and dexamethasone (DEX) were obtained from Duchefa and latrunculin B (LatB) from Sigma-Aldrich (MO, USA). Except PI ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Genomics 2020Quote: ... We followed the protocol as described previously [6] with one alteration: instead of using one paperdisc (Whatman qualitative filter paper, WHA1001070, SIGMA-ALDRICH CO LTD) per extraction ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were cultured for six days in 6-well-plates followed by treatment with the mTOR inhibitor temsirolimus (10 μM in DMSO; Sigma, St. Louis, USA) or Torin 1 (500 nM in DMSO ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were infected with the viruses in the presence of 6 μg/mL polybrene (Cat. #107689; Sigma-Aldrich, St. Louis, MO, USA). The overexpressing vectors were transfected into Ink4a/Arf-/- astrocytes using the NeonTM Transfection System 10μl Kit (Cat ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Four days after the intravenous injection of 106 iRBC into C57BL/6 mice pre-treated one day earlier with phenylhydrazine 97% (Sigma-Aldrich, 6.18µl/ml) 1 ml of fresh mouse blood was mixed with 10 ml of ookinete medium ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific; 50 μg/mL anti-β1, clone-mAb13, Millipore Sigma; 20 μg/mL anti-β4 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μM-sections were deparaffinized and antigen retrieval was performed in 10 mM citrate buffer (pH = 6; Sigma-Aldrich, St Louis, MO, USA) for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured on a 6-well to confluency and degradation was induced by adding 500 nM dTag-13 (Sigma-Aldrich, SML2601-1MG) or 500 µM auxin (Sigma-Aldrich ...
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bioRxiv - Zoology 2022Quote: The frogs underwent double euthanasia according to institutional ethics guidelines under ethics approval number NWU-00380-16-A5-01: first anaesthesia in 6% ethyl-3-aminobenzoate methansulfonate (MS222) (Sigma-Aldrich Co., USA) and then euthanasia through pithing.
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...