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Citations for Millipore Sigma :
9401 - 9450 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the glutamatergic nature of EPSCs was confirmed by a complete block of spontaneous activity with 6-cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX, 50 μM, Sigma-Aldridge, US) and D-2-amino-5-posphonopentanoic acid (APV ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... 6–8-week old B6D2F1 females were hormone primed by an intraperitoneal injection of pregnant mare serum gonadotropin (PMS, EMD Millipore) followed 46 hours later by an injection of human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich). Human serum albumin (0.1 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... at a concentration of 1.5 mM was sonicated in nanopore water with 3 mM lactic acid (Sigma-Aldrich, L1750, St. Louis, MO) and sterilized via passage through a 0.2 μm filter ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at room temperature and then precipitated from the supernatant by dropwise addition of ice-cold trichloroacetic acid (Sigma T069-100mL). Precipitated protein was spun down and washed twice with very-cold acetone (Fisher A18-500) ...
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bioRxiv - Cell Biology 2020Quote: ... media was aspirated from confluent cells and replaced with a minimal volume of serum free DMEM-F12 supplemented with 50 μg/ul L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902). Cells were left overnight at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... confluent cells were serum starved for 24 h in the presence of 50 μg/ul L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902). At the time of assay ...
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bioRxiv - Biochemistry 2020Quote: Several mL of a mixture consisted of 15% acetonitrile and 85% water with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich, USA) to pH 2.5 to suppress dissociation of labile protons were added to the dried extract ...
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bioRxiv - Neuroscience 2020Quote: R6/1 mice were treated (from 8 to 20 weeks of age) with vehicle (90% water, 10% Polysorbate 80) or betulinic acid (50 mg/kg; Sigma-Aldrich #855057) administered by oral gavage ...
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bioRxiv - Developmental Biology 2021Quote: ... dehydrated in 20% sucrose in PBS and cryosectioned at 8-10 μm thickness followed by Naphthol AS-BI phosphoric acid and diazotized Fast Garnet GBC stains (Fast green as counter stain) (Sigma Aldrich, 387A). For immunostaining ...
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bioRxiv - Molecular Biology 2022Quote: ... was induced in male C57BL/6 mice at 11–12 wk of age by a single injection of folic acid (240 mg/kg in vehicle, 0.15 mol/l NaHCO3, pH 7.4, Sigma-Aldrich, St. Louis, MO) (9 ...
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bioRxiv - Plant Biology 2019Quote: ... To determine and quantify these amino acids in plant extracts and enzyme assays standard materials were purchased from Sigma-Aldrich (Steinheim, Germany). Other chemicals were obtained in LC/MS grade from Roth (Karlsruhe ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was measured with a bicinchoninic acid (BCA) protein assay (Smith et al., 1985) using bovine serum albumin (BSA) as a standard (Sigma Chemicals, USA) with the EnVision microplate reader at an absorbance of 570 nm ...
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bioRxiv - Systems Biology 2019Quote: ... The resulting chromatogram and the mass spectra of the samples were analysed by comparing with those of amino acids standards (Sigma Aldrich, Germany). All the mass spectra and the chromatograms were analysed out on Thermo XCalibur 2.2 software (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... RtTA/Ngn2-positive iPS cells were plated as single cells at DIV0 onto nitric-acid treated coverslips coated with 50μg/mL poly-L-ornithine hydrobromide (PLO; Sigma-Aldrich #P3655-10MG) and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02 ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained for tartrate-resistant acid phosphatase (TRAP) to examine osteoclast activity and numbers (Sigma-Aldrich Kit, St. Louis, MO, USA), and Fast Green counterstaining and examined with a Nikon Eclipse 90i Advanced Research Scope ...
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bioRxiv - Molecular Biology 2019Quote: ... dissolved in 100 mL PBS containing Ca2+/Mg2+ and 25 mM N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES; Sigma, Vienna, Austria), resulting in a final collagenase concentration of 0.1 U/mL ...
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bioRxiv - Synthetic Biology 2021Quote: To induce the degradation of mAID-fused proteins, cells were treated with 500 µM indole-3-acetic acid (auxin, IAA, dissolved 500 mM in water) (Millipore Sigma, I5148) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... FBS supplementation was decreased to 3% FBS 3 days prior to the addition of 10□M Retinoic Acid (RA; Sigma-Aldrich, R2625). During differentiation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at a concentration of 1.5 mM was sonicated in nanopore water with 3 mM lactic acid (Sigma-Aldrich, L1750, St. Louis, MO) and sterilized via passage through a 0.2 μm filter as described in (5) ...
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bioRxiv - Bioengineering 2020Quote: ... collagen type I was extracted from rat tail tendons as freeze-dried sponges according previously published protocols.42,43 The extracted collagen sponges were dissolved in 0.02 N acetic acid (cat. no. 695092, Sigma-Aldrich, St. Louis, MO) to prepare a sterile stock collagen solution ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Microbiology 2021Quote: ... each spot was coated with 1.5 μL of a matrix solution consisting of saturated α-cyano-4-hydroxycynnamic acid or HCCA (C2020 Sigma, Lyon, France), 50% acetonitrile (CarboErba for HPLC ...
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bioRxiv - Microbiology 2020Quote: ... solution A from the kit and the supernatant was transferred into a new tube with one small spoon of glass beads (425-600 um, acid washed) (Sigma-Aldrich, USA). The samples were then vortexed three times in intervals of 3 seconds to break the cells ...
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bioRxiv - Microbiology 2019Quote: ... For metabolite extraction 1 ml 80% methanol containing 10 µM ribitol (RI, internal standard) were added to dried samples and for cell disruption 500 mg acid-washed glass beads (Sigma-Aldrich, USA) and a homogenizer (Precellys ...
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bioRxiv - Physiology 2021Quote: The NEFAS concentration was quantified using the Free Fatty Acid Quantitation Kit (MAK044) according to the technical bulletin (Sigma-Aldrich, United States). The results are expressed in ng/μl.
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bioRxiv - Plant Biology 2021Quote: ... was kept at 25°C and the elution was performed with acetonitrile (Optima, Fisher chemical, UK) and water containing formic acid (Sigma-Aldrich, Germany). A gradient of solvent A (H2O containing 0.1% formic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... used for prochloraz quantification was obtained from Rathburn (Walkerburn, Scotland), acetic acid (CAS; 64-19-7, LC-MS grade) from Sigma-Aldrich (Germany), 25:24:1 phenol:chloroform:isoamyl alcohol from Carl Roth GmbH & Co ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was removed and protein concentration was measured by use of a Bicinchoninic acid (BCA) solution (#B9643-1LSigma-aldrich, USA) coupled to copper II sulfate solution (#C2284-25ml, Sigma-aldrich, USA) following the manufacturer’s recommandations ...
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bioRxiv - Neuroscience 2022Quote: ... all samples were weighted and placed into 10 μL of ice-cold 0.1 M perchloric acid (Sigma Aldrich, St. Louis, MO, USA) solution with 100 ng/mL 3,4-dihydroxybenzylamin (DHBA ...
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bioRxiv - Neuroscience 2022Quote: ... water (for GC, HPLC, and spectrophotometry grade, Honeywell, Burdick, and Jackson, Muskegon, USA) and formic acid (≥98.0%) from Sigma-Aldrich (Steinheim, Germany) were used for UHPLC-HRMS ...
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bioRxiv - Neuroscience 2022Quote: ... 17-% methanol and was adjusted to pH 4.5 by phosphoric acid (all reagents were purchased from Sigma Aldrich, St. Louis, MO, USA), in line with 73 ...
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bioRxiv - Cell Biology 2022Quote: ... Essentially a colony of yeast is resuspended in 250ul of P1 buffer combined with 100 μl of acid-washed glass beads (Sigma G-8772) and vortexed for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... was used to prepare a stock solution of 10 mg/ml and filter sterilized) and lactic acid (Sigma, 11.4 M stock concentration). Collagen and lactoferrin were stored at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30%, Sigma-Aldrich, Germany) or A was 0.1% formic acid (for celesticetin detection ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 900 μl of 25% trichloroacetic acid (TCA) before harvesting precipitates on Whatman filter strips (Sigma-Aldrich, Cat: WHA1823035). Filter paper disks were placed in Biofluor scintillation fluid (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... Further tissues were homogenized using homogenizer on ice and subjected to deproteinization of sample adding 25μl Trichloroacetic acid (Sigma Aldrich, Catalog # T6399). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Microbiology 2022Quote: ... NC) or Brucella agar (Becton Dickinson, Canaan, CT) supplemented with 500uL of PBS with 0.1% taurocholic acid (Millipore-Sigma, St. Louis, MO) for C ...
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bioRxiv - Pathology 2022Quote: ... and is grown in Lysogeny broth (LB) or LB agar supplemented with 50 μg/mL of diaminopimelic acid (Sigma-Aldrich, Darmstadt, Germany) [63] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... L-ascorbic acid (A0278), ammonium iron (II) sulfate hexahydrate (215406), BSA (A3803), and β-nicotinamide adenine dinucleotide (NAD, N6522) were from Sigma-Aldrich. Tween-20 (10% ...
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bioRxiv - Synthetic Biology 2020Quote: ... The strains were precultured on Yeast Nitrogen Base (YNB) medium without amino acids and with 5 g/L ammonium sulfate (Sigma-Aldrich Y0626) and 10 g/L glucose ...
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bioRxiv - Neuroscience 2019Quote: ... A 10 µL microsyringe with stainless steel 33G beveled needle was filled with a 20 mM solution of kainic acid (KA; Sigma-Aldrich, France) in 0.9% sterile NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... or SD minimal medium or SD-Complete medium (60) containing leucine at 170 mg/l and all other amino acids (Sigma Aldrich, USA) at 85 mg/l.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml; Sigma-Aldrich, www.sigmaaldrich.com). We measured mass spectra using an Ultraflex III MALDI-TOF (Bruker Daltonics ...