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Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... they were first anesthetized using egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester) from Sigma-Aldrich. Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... 50-60 µm cryosections were blocked for 3h at room temperature in PBS containing 3% BSA (A7906, Sigma-Aldrich) and 0.5%Triton X-100 (T9284 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibodies (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... and the chocolate-flavored pellets were replaced with cotton pads infused with 1 ml of a 1% solution of 2,3,5-trimethyl-3-thiazoline (TMT, Sigma Aldrich) in water ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then washed with PBS 3 times before incubation with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257) in PBS at RT for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were rinsed and permeabilized in PBS-0.1% triton, 3 times 30 min, and incubated in blocking solution (PBS-0.1% triton, 1% donkey serum (D9663, Sigma)) prior to incubating with primary and secondary antibodies ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK), were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM ...
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bioRxiv - Systems Biology 2024Quote: ... UK) previously coated with calf- skin collagen (15 µg/cm2 and fibronectin 3 µg/cm2; both Sigma-Aldrich, UK). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNase A (345 μL TE, 3 μL 10 mg/mL RNAse A (Sigma-Aldrich, R6513), 2 μL 20 mg/mL Glycogen (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... paraformaldehyde in PBS and incubated for 30 min in blocking buffer containing 0.3% Triton X100 and 3% BSA (Sigma) in PBS to permeabilize the cells and block nonspecific staining ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed and permeabilized cells were then exposed to a blocking solution (3% bovine serum albumin (#A7906, Sigma‒Aldrich) in PBS ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Neuroscience 2024Quote: Stock solutions (1mg/ml) of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in N,N-dimethylformamide (Sigma-Aldrich 468495) were stored at −20°C ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating organotypic slices or vibratome sections were incubated in permeabilization solution (0.2% Triton-X100, 3% BSA in PBS; Sigma) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All the ASO probes were synthesized with biotin-TEG added to the 3’ end of the sequences (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... the culture medium was changed to medium supplemented with 500 μM 3-Indoleacetic acid (IAA; Sigma-Aldrich, I2886-5G) and 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... The cell nucleus was stained with DAPI (1:1000 dilution, Sigma-Aldrich 28718-90-3, St. Louis, MO, USA) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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Evolution of polyamine resistance in Staphylococcus aureus through modulation of potassium transportbioRxiv - Microbiology 2024Quote: ... Four of the cultures were diluted 1/100 into fresh TSB containing 2mM of spermine (Sigma, 71-44-3), and two of the cultures were diluted 1/100 into plain TSB as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Cell Biology 2024Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were maintained in the TREx BCBL-1-RTA medium with additional 3 μg/mL puromycin (P7255, Sigma-Aldrich) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin (SIGMA-ALDRICH #T7451 Clone 6-11B-1), α-tubulin (SIGMA-ALDRICH #T9026) ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Molecular Biology 2020Quote: ... The media was then carefully removed from the 10 cm dish and the enriched myoblasts population was seeded in a 6 well-plate coated with Matrigel (Sigma-Aldrich, St. Louis, MO, USA) in plating media ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Microbiology 2020Quote: Confluent cell monolayers grown in 96-well plates were pretreated with a range of concentrations of brefeldin A (BFA, EMD Millipore CAS 20350-15-6) or Golgicide A (GCA ...
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CSF1R inhibition by a small molecule inhibitor affects hematopoiesis and the function of macrophagesbioRxiv - Neuroscience 2020Quote: BMDM were primed with 150U/mL interferon-γ (IFNγ) for 6 hours followed by LPS at final concentration of 10ng/mL (Sigma-Aldrich, St. Louis, MO) added into culture medium for 20 hours ...
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bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
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bioRxiv - Neuroscience 2020Quote: ... and IL-6 in the conditioned media of WT and Akt2 KO mouse primary microglia cultures treated with LPS (200ng/ml, Sigma-Aldrich, St. Louis, MO, USA) and IFNγ (20ng/ml ...
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bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
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bioRxiv - Evolutionary Biology 2021Quote: ... The total amount of glucose was determined photometrically at 340 nm in a 96-well microplate photometer (HR 7000, Hamilton, Reno, NE, USA) after enzymatic conversion to gluconate-6-phosphate (hexokinase reaction, hexokinase from Sigma Diagnostics, St. Louis, MO, USA). NSC (Non Structural Carbohydrates ...
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bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Microbiology 2022Quote: ... quenched and 6-Carboxyfluorescein-filled liposomes were extruded through Whatman® Nuclepore™ Track-Etched Membrane (pore size=200 nm) (Sigma Aldrich, Saint Louis, MO) as previously described(Jimah et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were infected 12.5 µL of concentrated lentivirus pTRE-SadCas9-2xKRAB-dtTomato and 12.5 µL of concentrated lentivirus pCMV-rtTA in 400 µL of StemFlex medium supplemented with 6 ug/mL of polybrene (Millipore, Cat. No. TR-1003-G). After 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Neuroscience 2024Quote: Sensitivity to cold was assessed immediately following testing for mechanical paw withdrawal thresholds by applying a droplet of acetone (Sigma-Aldrich; approximately 5-6 μL) from the end of a blunt one C.C ...
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bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
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bioRxiv - Immunology 2023Quote: ... we removed the head kidneys from RNAlater and placed them in a tube with ∼10 ceramic beads (1.4 mm) and 600 µL of RTL Buffer enriched with 6 µL β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA) to avoid sample degradation by RNases ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... This solution contained 30 µl of scrambled or 10 µl VPS34-targeting shRNA per target sequence to a total of 30 µl and was supplemented with 6x polybrene to reach a final concentration of 6 µg / ml (Sigma Aldrich, Cat. # TR-1003-G) in a complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue sections were then trimmed to a ∼ 6 mm x 6 mm region centered about the implant site and the tissue block was embedded in a 4% agarose gel (Sigma-Aldrich, Saint Louis, MO, USA). This tissue-gel block was glued onto the slicing platform of a vibratome (VT 1000S ...