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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... juvenile or adult zebrafish were added to water containing 0.08 mg/mL Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich) until fish became non-responsive to touch (<5 minutes) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections of 3 μm in the thickness were conducted for Safranin O and fast green and TRAP (Sigma-Aldrich) staining ...
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bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... USA). 3- sulfopropionic acid (cat. #BD01044758) was purchased from BLD pharma. Tramiprosate (cat. # A76109) was purchased from Sigma Aldrich. GM1 and GM3 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Neuroscience 2023Quote: 17β-Estradiol (E2, E8875), β-Estradiol-3-Benzoate (EB, E8515) and Progesterone (P, P0130) were purchased from Sigma-Aldrich and dissolved in sesame oil ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml). All samples were washed with PBS and covered with aluminium foil until imaging.
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bioRxiv - Bioengineering 2023Quote: ... Nuclei were stained at room temperature for 3 minutes with DAPI (Sigma Aldrich, D9542, 1:500, 1 mg/ml).
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bioRxiv - Evolutionary Biology 2023Quote: ... medaka and zebrafish were deeply anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) and fixed by perfusion with 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... For the serial passaging MDCK cell were infected at a multiplicity of infection (MOI) of 0.0001 and incubated for 3 days at 37°C in DMEM containing TPCK-Trypsin (Sigma) at a final concentration of 1 µg/mL ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed with sterile PBS x 3 and cells were lysed with 0.25 mL of 1% (weight/volume) saponin (Sigma) for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Plant Biology 2023Quote: ... The negative masters were treated with a solution of 3-aminopropyltriethoxysilane (015-28251, Sigma-Aldrich, St. Louis, MO, USA) overnight under ambient conditions once before use ...
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bioRxiv - Cell Biology 2023Quote: ... and microtubules (1 µM paclitaxel, T7402, Sigma Aldrich; 33 µM nocodazole, M1404, Sigma Aldrich, 3 different commercial lots tested). The effect of nocodazole during overnight/short-term culture was enhanced by 10 µM verapamil (V4629 ...
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bioRxiv - Genetics 2024Quote: ... Zygotes with visible pronuclei washed 3 times in HTF medium and transferred to KSOM medium (Millipore, MR-106-D) and cultured in low oxygen chamber filled with the mixture gas (5% O2/ 5% CO2) ...
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bioRxiv - Neuroscience 2023Quote: ... Germany) containing the steroid hormone RU486 (200 μg/ml, CAS No.: 84371-65-3, Sigma-Aldrich, St. Louis, MO) for two days.
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bioRxiv - Biochemistry 2024Quote: ... and 3 µg standards (manually mixed, Avanti Polar Lipids® and Sigma-Aldrich®; BVO from Spectrum Chemical®) onto a Merck HPTLC glass plate silica gel 60 (20×10cm ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were treated with the respective concentrations of selected drugs (see Table 3) or DMSO (Sigma-Aldrich, Cat #:472301) as control and incubated for 6hrs and 24hrs before harvest for RNA extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... 1402-9500) containing 3 μL of SMART-seq3 lysis buffer (0.5 μL PEG 8000 (40% solution, Sigma Aldrich, P1458), 0.03 μL Triton X-100 (10% solution) ...
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bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
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bioRxiv - Immunology 2023Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Immunology 2023Quote: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 10 min at room temperature in the dark and washed 3 times with PBS (#D8537, Sigma). PBS was completely removed and cells were left to dry for 5 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Samples were concentrated to an A260ml−1 of 3 (100 nM) using an Amicon Ultra 0.5 ml spin column with a 100 kDa cutoff (Merck Millipore) to an OD260 absorbance of 5.0.
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Samples were measured in a 3 mm path-length cuvette (Hellma, 105.251-QS) in PBS (Sigma-Aldrich, D8537-500ML) containing 500 mM NaCl adjusted at pH 7.4.
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bioRxiv - Biophysics 2023Quote: ... the fluorescence spectra of mRaichu were measured in solutions containing 3 mg/mL BSA (SIGMA, Product No. 017-25771), 100 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3 kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Molecular Biology 2023Quote: Articular cartilage explants from the non-OA donors were treated with 3 μM of Wnt agonist CHIR99021 (Sigma-Aldrich) or with 1 μL/mL of its vehicle (V ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Plant Biology 2023Quote: ... 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC; >98%; Sigma-Aldrich, Buchs, Switzerland) diluted 50 fold in EtOH and sealed with screw caps containing a rubber septum ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled bone marrow stromal cells (BMSCs) were washed with 3 times with PBS and cultured in αMEM (Sigma, M2279) supplemented with 20% FBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 99.7% pure), Bisphenol TMC (Catalog #, 52746-49-3) and 2′,7′-Dichlorodihydrofluorescein diacetate (Catalog #, D6883) were purchased from Millipore Sig ma (MO ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...