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Citations for Millipore Sigma :
9401 - 9450 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Genetics 2023Quote: ... Proteins (5-15 μg) were separated on a 10% NuPAGE Bis-Tris Protein Gel and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked with 5% BSA made up in Tris-buffered saline-Tween 20 (TBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... The fibrinogen stock solution is made by first preparing a 50 mg/mL stock from lyophilized bovine blood plasma protein (Millipore). It is reconstituted in a controlled manner by adding sterile PBS without Ca2+ and Mg2+ and keeping it at 37°C without agitation for 2–3h ...
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bioRxiv - Developmental Biology 2023Quote: ... A small blood draw was carried out 4 weeks later to check progress and a booster injection of 100μg of Tin protein in 200 μl of Freund’s Incomplete Adjuvant (Sigma-Aldrich 344291) was carried out at four sites ...
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with a solution containing the membrane pore-forming protein Streptoylsin O (SLO) (Sigma catalog: S5265-25KU) sufficient to permeabilize 90% of HeLa cells ...
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bioRxiv - Immunology 2023Quote: ... the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore) to exclude the nucleic and cytoskeletal fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... the titrations of total protein were separated on 12% SDS-PAGE gel followed by blotting to Immobilon PVDF membrane (Millipore). Membranes were dried and total protein was stained with Sypro Ruby (Thermo-Fisher ...
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bioRxiv - Synthetic Biology 2023Quote: ... Dialyzed protein samples were concentrated using Amicon Ultra centrifugal filter units with a 10 kDa molecular weight cutoff (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... a molecular ‘anchor’ was added to cellular proteins by incubating the coverslips in a solution containing 1.4% Formaldehyde (Sigma F8775) and 2% Acrylamide (Sigma A4058 ...
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bioRxiv - Biophysics 2023Quote: ... Streptactin resin was washed with 40 column volumes (CV) wash buffer and SBP-tagged protein was eluted using wash buffer supplemented with 10 mM desthiobiotin (Sigma). Eluate was concentrated to 500 µl using a 100 kDa MWCO concentrator (amicon ...
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bioRxiv - Neuroscience 2023Quote: ... We used direct blue stains of total protein as loading controls by saturating PVDF membrane for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
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bioRxiv - Physiology 2023Quote: ... Lysates were collected by centrifugation and 500 μg of protein were incubated with 0.5 μl of Flag antibody (#F3165, Sigma-Aldrich) at 4 ℃ overnight ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... the purified proteins and domains were pooled and concentrated to 0.2–1 ml in PBS buffer by Amicon Ultra-15 (Millipore, USA). A BCA Protein Assay Kit (Beytotime Bio Co Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... and the soluble fractions containing the FLAG-tagged KDM2A protein were affinity purified using the anti-FLAG M2 affinity gel (Sigma). Gel beads were washed with TBS buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed twice with lysis buffer and proteins were eluted from the beads with 8 M urea (U5128; Sigma) and 0.1 M Tris–HCl at pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Immunology 2023Quote: ... The protein was then eluted in 15 μL of peptide elution buffer three times using Flag peptide or HA peptide (Sigma) 300µg/mL ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains BL21 (D3E)/ pRARE carrying the corresponding expression vectors were induced for protein expression with 0.5 mM isopropyl-1-thio-D-galactopyranoside (IPTG, Sigma-Aldrich) for three hours at 30 ° C ...
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bioRxiv - Bioengineering 2023Quote: ... The protein concentrator allowed to change the storage buffer to 100 µL of 0.1 M sodium borate (Sigma-Aldrich, HT1002), pH 9.3 ...
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... into the WT-CaM construct in the pET21a vector using standard PCR mutagenesis after residue D80 of the protein sequence using primers ordered from Sigma-Aldridge (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was achieved by incubating cell lysates containing 1 mg of protein overnight with anti-HA agarose beads (Sigma-Aldrich), Myc-agarose beads (Santa cruz biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... and overexpression of the proteins was induced by 0.1–0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma Aldrich, #I5502) for 20 hrs at 18°C for His6-tagged caltubin or 3 hrs at 30°C for GST-tagged caltubin ...
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bioRxiv - Immunology 2023Quote: ... 50 µg protein in 1× Laemmli buffer was separated by SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The PVDF membranes were blotted with primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... The next day the proteins were collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore) for size exclusion chromatography.
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bioRxiv - Biochemistry 2023Quote: Proteins guided towards high cysteine content were transformed into and expressed in Rosetta-gami B(DE3) Competent Cells (Novagen 71137). The 1 mL and 50 mL scale protein purification protocols were otherwise followed.
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bioRxiv - Biochemistry 2023Quote: ... cell lysates were normalized by dilution to a common protein concentration at 1 mL total volume and pre-cleared with 4B Sepharose beads (Sigma) at 4°C for 2 h while rocking ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 10 column volumes of FLAG buffer and proteins were eluted as 1 ml fractions with 100 μg/ml FLAG peptide (Sigma). 5 mM DTT was added to each fraction and peak fractions as determined from Coomassie blue gel electrophoresis were pooled and concentrated in Vivaspin 15-max speed filter (MWCO 30kD ...
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bioRxiv - Microbiology 2023Quote: Protein samples were resolved by SDS–polyacrylamide gel electrophoresis and transferred to Immobilon®-FL polyvinylidene fluoride membrane (Millipore Sigma) as previously described [76] ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected after centrifuging at 4°C for 10 minutes and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The fusion protein was cleaved using Thrombin (from bovine plasma, 50 NIH units/mg dry wt) following the manufacturer’s instructions (Sigma-Aldrich). The following day ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded on a NuPAGE 4-12% Bis-Tris protein gel and transferred onto an Immobilion PVDF membrane (Merk-Millipore) for 1 h at 35 V in a wet transfer apparatus buffered with 12.5 mM Trizma-Base (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
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bioRxiv - Immunology 2022Quote: ... Purified protein was concentrated and buffer exchanged into 1xDPBS using a 10kDa MWCO Amicon Ultra-15 centrifugal filter (Millipore Sigma), aliquoted ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Physiology 2023Quote: ... The proteins were collected using 700 μl elution buffer (20 mM Na2PO4, 300 mM NaCl, 300 mM imidazole) (Sigma Aldrich) and dialyzed against MOPS buffer (30 mM MOPS ...