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Citations for Millipore Sigma :
901 - 950 of 1467 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... A 2 mL volume of warm virus medium (1X MEM with 100U/100 μg/mL penicillin/streptomycin, 0.3% bovine serum albumin [Sigma]) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from CsCl purified reovius preparations (~1×1010 virus particles) using TRI Reagent LS (T3934, Millipore Sigma) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Each cell line was infected with varying volumes of concentrated virus in the presence of polybrene (Sigma Aldrich 107689) to determine the concentration that conferred survival in puromycin to 30% of cells ...
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bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... except that each virus was added to SVOG3e/rtTA cells and selected with 0.5 μg/ml Puromycin (Sigma P8833) and 0.6 mg/ml Geneticin to create polyclonal stable cell lines SVOG3e/rtTA/V5 (V5 control cell line) ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral mixes were created with an aliquot of virus into complete media and polybrene at 8μg/ml (Sigma-Aldrich) to have a target multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: Media was recovered approximately 48-hr post-transfection and cell-free virus stocks were generated by filtering the media through 0.45 µM filters (Millipore). The TZM-bl indicator cell line was used to quantify the amount of infectious virus(Derdeyn et al. ...
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bioRxiv - Biochemistry 2019Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Sigma) and oligo dT (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The activated cells were added to the virus-bound plates after removing dead cells using Histopaque-1119 (Sigma-Aldrich), and centrifuged at 3200 × g for 90 min at 32°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lentivirally transduced by spinfection with centrifugation of cells with virus in the presence of 8μg/ml polybrene (Millipore) at 1,000xg for two hrs ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded onto glass-bottom dishes and transduced with virus containing pEGFP_EB3 (gift from S. Godinho) Cells were treated with Eg5 (Kif11) inhibitor monastrol (67 μM, Sigma) for 2 hr ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RAMP1 expression achieved through transduction of virus containing RAMP1 MISSION TRC3 Open Reading Frame (ORF) plasmid (pLX_304) (Sigma, US) into RAMP2 KO-HUVECs ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... virus was mixed with 0.36 million cells in growth media supplemented with 8 μg/mL Polybrene (Sigma-Aldrich, H9268) and incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... The virus particles in the supernatant were concentrated to 100μl using Amicon® Ultra-15 Centrifugal Filters (Sigma-Aldrich Chemicals Private Limited ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Microbiology 2021Quote: ... cell monolayers were washed twice with PBS before adding virus inoculum in DMEM supplemented with 1μg/ml of trypsin TPCK (Sigma) and no FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were transduced with optimal volume of lentiviral virus at 50% confluence in MV2 medium and 8μg/ml Polybrene (Sigma). After 24 h ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Microbiology 2023Quote: ... The medium for the culture of influenza A virus in MDCK cells (virus medium) was prepared by supplementing Minimal Essential Medium with 4.3% bovine serum albumin (BSA; Sigma) and Normocin.
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Physiology 2024Quote: ... C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore) as shown previously40 ...
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bioRxiv - Genetics 2023Quote: ... RPE-1 cells were transduced with lentivirus at an MOI of 0.3-0.5 (usually between 50-100 µL of virus/well) and 10 µg/mL Polybrene (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Next the pseudotyped virus-containing culture medium was diluted 50% in culture medium supplemented with 8μgml−1 polybrene (Sigma) and immediately applied onto MutuDC1 cells ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transduced with the respective virus particles in an equal ratio using 5 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552, Sigma-Aldrich, Darmstadt, Germany) based on the p24 antigen concentration in the supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Virus-containing supernatants were harvested after 48 h transfection and filtered by syringe through a 0.45 μM filter (Millipore) to eliminate cell contaminates ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transduced with the virus for 48 h in the presence of 4 µg/ml polybrene (Sigma H9268).
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bioRxiv - Microbiology 2023Quote: ... neutralized whole-virus samples were incubated in D2O buffer (99% deuterium oxide, Sigma 151882, supplemented with 120 mM NaCl). 40 μL D2O buffer was added to every 10 μL of neutralized virus (final D2O concentration 78% ...
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bioRxiv - Cancer Biology 2023Quote: ... subconfluent cultures were incubated with virus-containing supernatants in the presence of 4µg/ml polybrene (Sigma-Aldrich , TR-1003) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Cancer Biology 2024Quote: ... Virus transduction of target sarcoma cells was performed in the presence of 8.0 μg/mL polybrene (Sigma-Aldrich, #H9268) and puromycin selection (3 μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1×107 OE19 cells were treated with virus (MOI 0.7) and 5 μg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 500 ng/μL puromycin (Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... the virus containing supernatant was centrifuged 500 x g for 5min and filtered by using 0.45um PES filters (Millipore). The lentivirus was concentrated by using Lenti-X concentrator (Clontec) ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...