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Citations for Millipore Sigma :
951 - 1000 of 1467 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 2% HI-FBS PBS with 1 µg/mL DAPI (Sigma Aldrich, #D9542) to stain dead cells ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were stored at –20 °C and insulin secretion was quantified using human (Millipore Cat. # HI-14K) insulin radioimmunoassay kits.
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against FLAG tag [M2] (F1804, RRID: AB_262044) and [M2] (F3145, RRID:AB_259529) were purchased from Sigma Aldrich. Antibodies against DDX3X [D19B4] (8192S ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in phosphate-buffered saline (PBS) containing 1/400 anti Rho-tag antibody 4D2 (Millipore), 15mM NaN3 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the eluate was analysed by SDS-PAGE and western blotting with anti-His6-tag antibody (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... respectively) was cloned into a pRSF-Duet vector preceded by an N-terminal His6-SUMO tag (Novagen)[11 ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoblots from tandem affinity purifications were probed with antibodies against S-tag (mouse monoclonal MAC112; EMD Millipore) followed by visualization using IRDye-tagged secondary antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... The upper portion of the membrane was probed with anti-HA tag antibody (mouse, Sigma-Aldrich, H3663) diluted 1:1,000 in 5% skim milk solution ...
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bioRxiv - Biochemistry 2020Quote: ... Phage with uncleaved FLAG-tag were removed by immunodepletion using M2 anti-FLAG monoclonal antibody (Sigma-Aldrich) coupled to epoxy-activated M-450 magnetic beads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: TAG and cholesterol evaluation of tissue and serum were processed according to the manufactures’ instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). E ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Biochemistry 2024Quote: ... GFP variants were cloned downstream of a 7xHis-SUMO tag in a modified pET22b vector (EMD Millipore). Tag variants were generated by ligating synthetic dsDNA oligos (IDT ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... were transduced when reaching around 50-70% confluence with 1x concentrated virus diluted in ADF+++ and supplemented with hEGF and 5 µg/µl polybrene (Sigma). The cells were incubated for 12-18 h with the viral suspension ...
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The parental HEK293T cells were transduced with this virus and the HEK293T-ACE2 cell line was selected and maintained with medium containing puromycin (Sigma). ACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells’ media was exchanged with virus-containing supernatant at 48- and 72-hours post-transfection with 8µg/ml polybrene (Sigma #H9268). N2a cells were expanded and FACS sorted for mCherry+ cells.
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bioRxiv - Biochemistry 2020Quote: ... The medium was replaced after 72 h and cells containing the integrated virus were selected with Puromycin (50ng/μl) (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... the assay was performed on cells exposed to Pronase (3,5mg/ml) or using virus pre-exposed to 1 to 100 U/mL heparin (Sigma-Aldrich). The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...