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Citations for Millipore Sigma :
801 - 850 of 1384 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
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bioRxiv - Immunology 2020Quote: ... and cells were overlaid with virus growth medium containing Avicel microcrystalline cellulose (final concentration of 1.2%) (Sigma-Aldrich). The plates were then incubated at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in 1.5 mL virus-containing media supplemented with 0.5 mL fresh media and 0.8 μg/mL polybrene (Millipore Sigma). The 6-well plate was covered with aluminum foil to keep cells in the dark ...
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bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag antibody (1:1000 dilution used) raised in mouse (F1804) was obtained from Sigma-Aldrich and Porin antibody (1:5000 dilution used ...
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bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
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bioRxiv - Microbiology 2019Quote: ... The hexa-histidine tag was removed overnight with thrombin (5 U thrombin (Sigma-Aldrich, Darmstadt, Germany) per mg SiiA protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Biochemistry 2021Quote: Monoclonal antibodies against the Flag tag epitope and Cdh1 were purchased from Sigma (St. Louis, MO) while antibodies against GFP ...
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bioRxiv - Biophysics 2020Quote: ... MSP1D1 was purified using IMAC72 with further cleavage of 6xHis-tag by TEV protease (Sigma-Aldrich). The lipid mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Molecular Biology 2022Quote: Full-length REC114 and its variants were cloned as Strep-tag fusions into pRSFDeut-1 (Novagen). TOPOVIBL 1-385 was cloned as a 6xHis-SUMO fusion protein in pETM11 ...
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bioRxiv - Biochemistry 2019Quote: ... The hexahistidine tag was removed by thrombin cleavage by incubating with 1:5000 thrombin (EMD Millipore) overnight at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... They included Fluidigm CS1 and CS2 tags and were ordered from Sigma (see appendix table 3). Reactions were performed in multiplex primer groups with primers each at 1 μM concentration (group 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Cell Biology 2021Quote: The flag-tag immunoprecipitations were carried out using FLAG-M2 coated agarose beads (Sigma-Aldrich #F2426) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation with primary antibodies against Strep Tag II Antibody HRP conjugate (Sigma, 1:5000), anti-FLAG Antibody (Sigma F7425 ...
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bioRxiv - Microbiology 2023Quote: ... ORF3c-HA was detected using a primary antibody against the HA-tag (Sigma-Aldrich, Cat# H3663) followed by staining with a secondary AF555 antibody (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Flag-Tag (F1804, 1:2,000 for Western blot, 1:200 for immunofluorescence) was from Sigma-Aldrich. Anti-mouse IgG–HRP (7076S ...
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bioRxiv - Cell Biology 2023Quote: ... HA tag sequence (YPYDVPDYA) was introduced by linker insertion at the 5’ side of pET28a (Novagen) MCS to generate pET28-HA-Nter ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA tag-PLUS PLA probe and anti-rabbit IgG-MINUS PLA probes (DUO92005: Sigma-Aldrich) appropriately diluted in the Duolink antibody diluent were applied onto the specimens that were incubated for 60 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were probed with primary antibodies against FLAG-tag (dilution 1:1000, mouse; Sigma, A1804), Wee1 (dilution 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Western blotting for the 6xHis-tag was performed using either HRP-conjugated primary antibody (Sigma, rabbit) or non-HRP-conjugated antisera (rabbit).
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bioRxiv - Microbiology 2024Quote: ... and incubated with a mouse monoclonal antibody [M2] specific to the FLAG-tag (Sigma-Aldrich, F1804) in 1% BSA/PBS at 37℃ for 1 hour ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).