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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and pMCAo-21d group received 4 intraperitoneal injections of 50 mg/kg of 5-Chloro-2′-deoxyuridine (CldU, Sigma). The injections were administered 6 ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... methyl triflate and 2-ethyl-2-oxazoline were obtained from Sigma Aldrich and dried over CaH ...
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bioRxiv - Neuroscience 2021Quote: The mGluR5 specific negative allosteric modulator CTEP (chloro-4-((2,5-dimethyl-1-(4-(trifluoromethoxy)phenyl)-1H-imidazol-4-yl)ethynyl)pyridine) was purchased from Sigma-Aldrich (SML2306) and administered at 2 mg/kg concentration by intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 days at 4°C with rabbit anti-AVP (1:2,000, Sigma, PC234L) or rabbit anti-Ntng1 (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged at 1/8 ratio every 3 or 4 days with Accumax (Millipore, ref. SCR006).
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bioRxiv - Plant Biology 2021Quote: ... 600 μl 4% acetic acid with 20 μl 1mM phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX, Sigma) and 0.6 μl 1mM spike control 8-Br-2′,3′-cAMP (Biolog ...
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bioRxiv - Microbiology 2022Quote: ... XTT (sodium 3′- [1- (phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4- methoxy6-nitro) benzene sulfonic acid hydrate) (Sigma-Aldrich), according to the manufacturer’s recommended specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... 2’-O-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP; Sigma-Aldrich), and 0.1 mM 3-isobutyl-1-methyl xanthine (IBMX ...
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bioRxiv - Immunology 2023Quote: ... 5 ml of PBS and 5 ml of 4% Dextran (Sigma-Aldrich, 31392-50G) in PBS (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... The CGCaP composites were then crosslinked via carbodiimide chemistry in a PBS solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma-Aldrich) and N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated medium and flowthrough medium were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Animal Behavior and Cognition 2022Quote: Ethanol 95% (v/v) and (-)-nicotine hydrogen tartrate salt ((-)-1 -Methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt were purchesed by Sigma Aldrich (St. Louis, MO, USA). The CB1/2 receptor agonist (R)-(+)-[2,3-Dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone mesylate ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Neuroscience 2024Quote: ... The medium was changed every 3–4 d and enriched with granulocyte-macrophage colony-stimulating factor (5 ng/ml GM-CSF, Sigma-Aldrich). After approximately 14 d at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... 3-4 µL was separated using a Millipore SeQuant ZIC-pHILIC (2.1 × 150 mm, 5 µm particle size, Millipore Sigma #150460) column with a ZIC-pHILIC guard column (20 x 2.1 mm ...
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bioRxiv - Bioengineering 2021Quote: ... Initiator 2,2’-azobis(2-methyl-propionitrile) (AIBN; Sigma, >98%) was recrystallized from methanol (MeOH ...
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bioRxiv - Neuroscience 2021Quote: ... and on day 5 naloxone hydrochloride (1 mg/kg, s.c. Sigma-Aldrich, St. Louis, USA) was administered 1 hr after the morning opioid injection ...
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bioRxiv - Developmental Biology 2024Quote: 4-Hydroxytamoxifen (4-OHT) Treatments and Bromodeoxyuridine Incorporation: 4-OHT (T176, Sigma) treatment was performed as previously described (Mosimann et al. ...
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bioRxiv - Cell Biology 2023Quote: ... zymosan (life technologies, added at a 1:5 ratio) or R848 (Sigma-Aldrich; 4 μg/ml). After this time ...
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bioRxiv - Cell Biology 2022Quote: ... 1mM tris (2 carboxyethyl) phosphine hydrochloride (TCEP) (Sigma #C4706) and protease inhibitors (as above) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1mM Tris(2-Carboxyethyl)phosphine hydrochloride (Sigma-Aldrich) (Buffer A) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg/ml isoprenaline hydrochloride (I5627-5G; Sigma-Aldrich), 24 μg/ml adenine (A8626 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 μg/mL doxycycline hydrochloride (Sigma-Aldrich, D3072) and plated in hESC-Qualified ...
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bioRxiv - Immunology 2022Quote: ... 6-diamino-2-phenylindole hydrochloride (DAPI) (Sigma-Aldrich, USA). Stained cells were visualized under confocal microscope (ZEISS LSM 800 ...
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bioRxiv - Genomics 2024Quote: ... 2 μg/mL doxycycline hydrochloride (Sigma; Cat.No. D-9891), 5 μg/mL Mouse Laminin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5 mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... reduced by tris(2-carboxyethyl)phosphine hydrochloride solution (Sigma) and alkylated by iodoacetamide (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Cell Biology 2021Quote: ... Partial depletion of cholesterol was achieved by incubating the cells in 4 mM methyl-β-cyclodextrin (MβCD, Sigma Aldrich: 332615) at 37°C for 30 min or in 60 μg/ml saponin (Nacalai Tesque ...