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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the expression of the protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG – Sigma) and the flask was placed at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2022Quote: ... LATS1 kinase was immunoprecipitated from 500 µg of protein lysates and subject to Oxyblot assay (#S7150, Millipore) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Separated proteins were wet transferred onto 0.45mm pore-sized PVDF membranes (Immobilon®-FL PVDF membrane, Millipore) for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The presence of proteins in samples was detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich, France). Acquisition was performed on a ChemiDoc Imaging System (Biorad ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Immunology 2022Quote: ... Equivalent amounts of protein were separated by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Membranes were incubated with antibodies per manufacturer’s suggestions ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Immunology 2022Quote: ... Purified proteins were concentrated using a 100 kDa and 30 kDa cutoff concentrator (EMD Millipore, Burlington, MA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electroblotted from 10% acrylamide gel to nitrocellulose membrane (Immobilon-P, MILLIPORE Corporation, Bedford, MA, USA) after the separation of SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... magnetic protein A/G beads were prepared with 5 µg of rabbit anti-AGO2 (Millipore, #03-110) or normal rabbit IgG antibodies (from the Magna RIP kit) ...
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bioRxiv - Physiology 2023Quote: Biosynthetic labelling of newly synthesised proteins was achieved by oral consumption of deuterium oxide (Sigma-Aldrich, UK), consistent with our previous work (Camera et al. ...
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bioRxiv - Plant Biology 2022Quote: ... and transferred to a PVDF membrane followed by probe with Phos-tag BTL-111 according to the manufacturer’s instructions (www.wako-chem.co.jp) to detect protein phosphorylated levels and anti-FLAG®M2-Peroxidase (HRP) (Sigma) antibody to detect the total amount of immunoprecipitated TMK1-FLAG.
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bioRxiv - Neuroscience 2023Quote: ... or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... These protein samples were resolved on 10% SDS-polyacrylamide gels and transferred to Immobilon-P membranes (Millipore) using the semidry method (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
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bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... SDS-polyacrylamide gel (10%) was cast in the presence of 0.5 mg/ml myelin basic protein (Sigma) within the gel ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using an Amicon Ultra 100 K filter (Merck Millipore, Billerica, Massachusetts, USA) before further purification using Superdex 200 gel filtration (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting eluate was collected and used for total protein isolation using Trichloroacetic acid-TCA (Sigma, T0699). The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Then T24 chromatin’s DNA and proteins were separated by adding 0.5 µl Benzonase (Sigma Aldrich, E8263-5KU) and vortexing ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were concentrated to a final concentration of ∼3 mg/ml by using Centricon filters (Millipore), aliquoted and flash-frozen in liquid nitrogen for −80°C storage ...
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bioRxiv - Bioengineering 2024Quote: ... Glycosidases were removed after incubation by filtration through 10 kDa protein-binding EZ™ filters (Millipore Corporation). N-glycans were then analysed by UPLC as previously described (36).
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bioRxiv - Biochemistry 2024Quote: ... The coding sequence of the DDX1 protein was PCR amplified and inserted into the pET28 vector (Novagen). A His-tag was placed in the N-terminal of DDX1 and a stop codon was inserted at the coding sequence of amino acid 708 ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the protein of interest were pooled and concentrated in an Amicon centrifugal unit (EMD-Millipore) with the appropriate molecular weight cutoff and purified on an AKTA FPLC system (Amersham Biosciences Co. ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were loaded onto a 10% SDS-PAGE gel and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 3% BSA (BioFroxx ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged for 15 min at 20 000 g and protein concentration was assessed using Bradford assay (Sigma). 50 µg of protein for each sample was transferred in fresh tubes ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the WT SCO4122 and mutant M93L-SCO4122 proteins were transferred on to polyvinylidene difluoride (PVDF) membrane (Millipore) using transfer buffer (6g Tris base ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies against EI24 (Sigma) ...
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bioRxiv - Genetics 2024Quote: ... Proteins were separated in SDS-polyacrylamide gel and transferred onto nitrocellulose membrane (Amersham Protran, GE10600001, Sigma Aldrich) by wet transfer method ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein G magnetic beads (TAMAGAWA SEIKI, TAS8848N1173) were pre-incubated with anti-FLAG antibody (SIGMA [M2], F1804) for more than 1 h at 4°C with gentle rotation before adding lysate ...
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bioRxiv - Developmental Biology 2024Quote: ... TBX33xFLAG proteins were detected by chemo-luminescence using monoclonal M2 anti-FLAG antibodies (1:500, Sigma, F1804) in combination with donkey anti-mouse IgG-HRP antibodies (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... The supernatant collected for protein purification was purified with Centricon® centrifugal filter (10 K NMWL, Millipore). Concentrated IFN proteins were verified by western blot.
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bioRxiv - Immunology 2024Quote: Equal amounts of protein extracts were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). Following a 2-hour blocking period in 5% skim milk in TBS-T buffer [20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were concentrated to a final concentration of ∼3 mg/ml by using Centricon filters (Millipore), aliquoted and flash-frozen in liquid nitrogen for −80°C storage ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated in an SDS-PAGE 10% gel and subsequently blotted onto a PVDF membrane (Millipore). After incubation with blocking buffer (Tris-buffered saline with 5% nonfat milk) ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions with target proteins were pooled and concentrated using an Amicon Ultra 3K NMWL (Merck Millipore). The sample was ultracentrifuged (45,000 rpm x 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... the purified native protein was first concentrated to 8 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then mixed with 2 mM ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein peak was collected and concentrated using a 10 kDa Amicon Ultra 15 concentrator cell (Millipore), and then applied to a Superdex 200 16/600 pg size exclusion column equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... 4°C and precleared by incubating with a mix of Protein G Plus/A Agarose Beads (Millipore) on a tube rotator for 2-3h ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred onto a polyvinylidene difluoride (PVDF) membrane (Sigma). Membranes were blocked in Odyssey blocking buffer (Li-cor Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets were resuspended in 200 µL lysis buffer (1X BugBuster® Protein Extraction Reagent, EMD Millipore; 0.025 U/µL Benzonase® Nuclease ...
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bioRxiv - Microbiology 2023Quote: ... the reduced proteins were transferred to a 30 kDa Microcon filter unit (YM-30 filter units, Millipore) and centrifuged at 14,000 × g for 20 min in all consecutive steps ...