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Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 1 mg protein was incubated with 50 μl equilibrated anti-FLAG M2 affinity gel (Sigma–Aldrich, A2220) on a rotating wheel for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins (25-50µg) were resolved on 8 to 15% SDS-PAGE and blotted onto PVDF membrane (Millipore). Membranes were blocked for 1 h at room temperature with TBS-0.1% Tween20 and 5% milk or 1% bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
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bioRxiv - Immunology 2020Quote: ... then 25 ug total protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and probed with the indicated primary antibodies following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were run on SDS-polyacrylamide gel and transferred to Immobilon-P membrane (Millipore). Membranes were incubated with anti-YAP (Novus) ...
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bioRxiv - Molecular Biology 2022Quote: ... The centrifuged protein pellet was then resuspended in a urea/thiourea buffer containing protease inhibitors (PefaBloc; Sigma) prior to reduction with dithiothreitol and alkylation with iodoacetamide ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the BCA reagent according to the directions of the supplier (Sigma-Aldrich). Following β-mercaptoethanol (1% ...
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bioRxiv - Neuroscience 2021Quote: Both monomeric and fibrillar proteins were desalted first using the Amicon Centrifugal Filter (Millipore Sigma, Burlington, MA) before analyzed by ESI-MS ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C) and then incubated with pre-blocked Protein G beads (30ul of 50% slurry) (Sigma, USA) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
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bioRxiv - Immunology 2022Quote: ... All cells were incubated for 72 hours with Myelin Oligodendrocyte Protein (MOG) (2.5ug/ml; Sigma-Aldrich, M4939), with or without rIL-12 (25ng/ml ...
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bioRxiv - Genomics 2019Quote: ... Clarified lysates were incubated overnight with Protein A dynabeads conjugated to mouse anti-FLAG (Sigma Aldrich F3165) or anti-IgG antibody overnight at 4C ...
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bioRxiv - Immunology 2021Quote: ... 50μL of RIPA buffer for protein extraction ready-to-use-solution (Sigma-Aldrich®, Product No. R0278) containing Triton X100 1% and SDS 0.1% was added to the apical surface of AECs and incubated for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore, Billerica, MA, USA) at 4,830× g at 4 °C for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Effects on protein synthesis rates were assessed by SDS-PAGE and immunoblotting using puromycin antibody (Millipore, #MABE343).
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bioRxiv - Microbiology 2019Quote: ... The protein content was measured using the BCA Kit (Bicinchoninic Acid, Sigma-Aldrich, St Louis, MO, USA) and bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2019Quote: ... following which the proteins were transferred from gel to membrane and probed using Anti-FLAG antibody (Sigma).
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... the buffer matrix for the protein standards was changed by adding 1% BSA (A7030, Sigma Life Science) to achieve a higher matrix complexity ...
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bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were resolved by SDS-PAGE and analyzed by western blot using anti-Myc (Grc3; EMD Millipore), anti-Flag (Las1 ...
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bioRxiv - Microbiology 2019Quote: ... The eluted protein was concentrated using a 10 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The concentrated protein was further purified using size-exclusion chromatography with a Superdex 75 10/300 GL column (GE Life Sciences ...
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bioRxiv - Genomics 2019Quote: ... The dialyzed protein solution was concentrated using an Amicon Ultra-4 Centrifugal Filter Units 30K (Millipore UFC803024), and sterile glycerol was added to make a final 50% glycerol stock of the purified protein.
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bioRxiv - Physiology 2020Quote: ... Equal quantities of proteins were resolved by SDS-PAGE gels and blotted onto polyvinylidene difluoride membranes (Millipore). Then ...
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bioRxiv - Microbiology 2019Quote: ... The purified proteins were concentrated to 30-40 mg/mL using Amicon concentrators (30-kDa cutoff; Millipore), aliquoted ...
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bioRxiv - Genomics 2019Quote: ... DENV C protein purified fractions were concentrated with a Centriprep® Centrifugal Filter (Millipore, cutoff of 3kDa) and stored at −80 °C ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Total protein (30 μg) was separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck, Germany) using a transfer apparatus (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Microbiology 2019Quote: ... Equal amounts of total protein were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). The membrane was then blocked with horse blocking buffer (bioWORLD ...
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bioRxiv - Genetics 2020Quote: ... Resin-bound proteins were eluted in R buffer containing 300 mM NaCl and 40 mM glutathione (Sigma). The sample was supplemented with 0.2 µg/mL of HRV-3C protease (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: Proteins separated by SDS-gel electrophoresis (7.5–15% polyacrylamide) were transferred to Immobilon-PSQ PVDF membranes (Millipore). After blocking with 5% non-fat dry milk in TBS (50 mM Tris·HCl ...
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bioRxiv - Systems Biology 2020Quote: ... One part was stained using Coomassie Blue for total protein content (Brilliant Blue G solution, Sigma Aldrich) and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Immobilon-P, Millipore). After being incubated with the primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants containing untagged protein were concentrated using Centriprep centrifugal filter devices (Millipore; molecular weight cutoff, 10 kDa) and loaded on superdex 75 column on an AKTA pure 25L protein purification system (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 centrifugal filters (3 kDa molecular weight cut-off, Millipore). Reducing agent (2 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma, P3775, 1:10,000) and anti-Flag (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 10 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
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bioRxiv - Biochemistry 2021Quote: ... Each well contained 0.5 mg/ml protein and 2.5X SYPRO orange dye (Sigma, St. Louis, MO, USA) in 10 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... After thawing the extraction buffer protein was precipitated with 100% trichloroacetic acid (final concentration 10%)(Sigma-Aldrich). After centrifugation at 12000 g for 5 min the pellet was washed with ice-cold 80% ethanol and ice-cold acetone by successive centrifugation-resuspension cycles ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The sample was then added to 75 μL of pre-washed Protein A sepharose beads (Sigma-Aldrich) and further incubated with rotation at 4 °C for an additional 30 min ...