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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... the column was inverted and bound proteins were eluted with 10 ml of buffer PT containing 0.1 mg/ml Flag peptide (Sigma). The eluate fractions were dialyzed against buffer A [25 mM Hepes-NaOH (pH 7.5) ...
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bioRxiv - Cancer Biology 2020Quote: ... Histone H3 lysine 27 trimethylated protein was detected using an anti-H3K27me3 antibody (rabbit polyclonal, Millipore, Burlington, MA, USA) diluted 1:500.
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bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with endoproteinase Lys-C (Wako, Osaka, Japan) and sequencing grade modified trypsin (modified sequencing grade, Sigma) followed by quenching with 1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2020Quote: HMLE variants were washed with PBS prior to protein extraction with lysis buffer (PBS, protease inhibitor cocktail (Sigma-Aldrich), phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... Total protein concentration was determined using the Bradford assay with bicinchoninic acid (BCA) reagent (Millipore Sigma, St. Louis, MO). Total protein (40 μg ...
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bioRxiv - Microbiology 2021Quote: ... Extracellular proteins (10 μg/mL; totally 700 mL) were filtered through a 0.22-μm nitrocellulose membrane (47-mm diameter; Millipore) and concentrated using the Amicon Ultra Centrifugal Filters to 7 mL (cut-off of 30 kDa) ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells grown on 6-well culture plates using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Biochemistry 2021Quote: ... SEC purified protein-DNA preparations were concentrated 10-fold by ultrafiltration (Amicon Ultra-15 30K centrifugal filter device, Millipore) and subjected to crystallization.
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... prelimbic cortex were dissected and homogenized in lysis buffer (pH 7.4) containing a cocktail of protein phosphatase and proteinase inhibitors (Sigma). The samples were centrifuged at 14,000 r.p.m ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were immune precipitated for 2.5 hours at 4°C using Ezview anti-HA agarose (Sigma-Aldrich) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 16,000 g for 10 min and protein concentration was normalized by Amidoblack staining (Sigma). Western blotting was performed following standard protocols as described below ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... the alpaca was boosted twice with 250 μg S protein in 250 μl Freund’s incomplete adjuvant (F5506-10ML, Sigma). One week following the 2nd immunization ...
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bioRxiv - Immunology 2020Quote: For immunoblot analysis, the M-PER Protein Extraction Reagent (Pierce, USA) supplemented with a protease inhibitor cocktail (Sigma, USA) was used to lyse the cells ...
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bioRxiv - Molecular Biology 2021Quote: Protein depletion was induced by treating cells with a final concentration of 500 μM IAA (I5148-10G, Sigma-Aldrich). Time series experiments were performed by inducing protein degradation at different time points and collecting all samples at the end of the time course.
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore) with a 50 kDa molecular weight cut-off ...
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bioRxiv - Immunology 2021Quote: ... and pSARS-CoV-2 expressing SARS-CoV-2 S protein (0.6ug) (GenBank; MN908947.3) (Brouwer et al., 2020).Transfection was performed in 293T/17 cells using genejuice (Novagen, USA) transfection kit according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Genetics 2020Quote: ... Protein samples were subjected to SDS-PAGE and wet-transferred to a 0.2 μM Immobilon-PSQ PVDF membrane (Millipore) at 200 mA for 90 minutes ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Genetics 2020Quote: ... The purified covalent protein-DNA complexes were concentrated to about 0.1– 0.2 mM using Ultracel®-10K Centrifugal Filters (Millipore) in a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
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bioRxiv - Molecular Biology 2020Quote: ... Dialyzed DENV2C protein was concentrated using Amicon Ultra-4 Centrifugal Filter Units (10 kDa molecular weight cut off, Millipore). The concentration of the purified protein sample was determined using nanodrop.
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bioRxiv - Physiology 2020Quote: ... Membranes were washed in TBS-T and proteins were visualized using a chemiluminescent substrate (Sigma Aldrich, Oakville, ON, Canada).
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
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bioRxiv - Biochemistry 2022Quote: ... then complexes immunoprecipitated using the monoclonal antibody 13D11 against the B-subunit followed by Protein A Sepharose beads (Sigma). After elution with cracking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μg of protein in 25 μl of 100 mM NH4HCO3 were reduced with 2.5 μL of 200 mM DTT (Sigma) at 90°C for 20 min and alkylated with 10 μL 200 mM iodoacetamide (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
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bioRxiv - Biophysics 2022Quote: ... Purified Vnucfree solubilized in 0.03% n-DDM was mixed with the lipid stock and membrane scaffold protein MSP1E3D1 (Sigma) at a specific molar ratio of enzyme ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was concentrated to ~ 450 μM of protein using Amicon Ultra Centrifugal Filters unit 3KDa cutoff (Millipore Sigma). Crystals were grown at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein solution was concentrated to 5 mL in a 10 kDa molecular weight cutoff centrifugal ultrafiltration device (Millipore) and loaded onto a Sephacryl S100 HR 26/60 size-exclusion chromatography column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... and mNedd4△N52 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by electrophoresis through 12% polyacrylamide gels containing SDS (0.1%) and transferred to a PVDF membrane (Millipore). The membrane was blocked with 2% (w/v ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Fab protein was eluted with 50 mM acetate buffer at pH 5.0 and concentrated using Ultra-15 Centrifugal Filters (Millipore). Protein concentration was determined using the Qubit protein assay (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence for the mature STIC2 protein (aa 49-182) was cloned into BamHI/SalI linearized pETDuet1 (Novagen) and pGEX4T3 (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... After concentrating the protein using a 30 kDa molecular weight cut-off Amicon Ultra-15 centrifugal filter unit (Millipore) the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare ...