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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 30µg to 50µg of total proteins were loaded onto 7.5% or 12% SDS-polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin or non-fat milk prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescent proteins encoded on the plasmid also carries a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Cells were cultured in autoclaved HL5 medium (per L ...
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bioRxiv - Genetics 2022Quote: ... 25 μg chromatin was diluted with IP dilution buffer and pre-cleared with Protein A agarose beads (Merck-Millipore) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: The protein concentrations of the lysates were determined by an amido black filtration assay56 with Fraction V BSA (Sigma) for a standard curve ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Molecular Biology 2020Quote: ... A 20 μl sample of the cleared protein was mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) and diluted in 250 μl 1.25× protein loading buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Unbound fiber knob protein was removed by washing twice in cold PBS and primary CAR RmcB (Millipore; 05-644) or primary CD46 MEM-258 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted in 7 aliquots of 700 μl each with elution buffer (10 mM reduced glutathione (Sigma 4251-5G in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
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bioRxiv - Cell Biology 2022Quote: ... were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
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bioRxiv - Immunology 2022Quote: ... Both multivalent proteins were buffer exchanged to remove excess imidazole and concentrated with 10 kDa cutoff centrifuge filters (Millipore), then filter sterilized by 22 μm centrifugal sterile filter (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates (25 μg) were resolved by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Sigma-Aldrich, IPVH00010). Membranes were blocked in 5% nonfat milk in TBST buffer at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Biophysics 2019Quote: ... lyophilized protein was dissolved in sodium phosphate buffer (pH 7.4) and filtered using 0.22 μm low protein binding membranes (Millex-GP, Merck Millipore, Germany). The protein concentration was determined by the measurement of absorbance at 277 nm using the extinction coefficient 5960 cm-1M-1.
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 600 µl of a 1.6 mg/ml total protein lysate were incubated with 30 µl Anti-FLAG M2 magnetic beads (Sigma) at 4°C while rotating for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were separated on a SDS-PAGE gel at an acrylamide percentage appropriate to protein size and transferred onto a polyvinylidene difluoride membrane (PVDF, 0.45 μm Millipore). The membrane was blocked in 5 % milk in PBS 0.1 % Tween 20 (PBS-T ...
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bioRxiv - Biophysics 2019Quote: ... after which the protein-bound stain was dissolved in 150 µl of 10mM Tris-base (Sigma-Aldrich, Zwijndrecht, Netherlands). The optical density was read at 540 nm with the HTX Synergy microplate reader (BioTek ...
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bioRxiv - Neuroscience 2019Quote: A total mass of 600μg brain protein was subjected to reduction with 10mM Tris(2-carboxyethyl)phosphine(TCEP, Sigma) at 30°C for 2hrs and then alkylation with 20mM IAA containing 100mM ABB in the dark at room temperature for 30mins ...
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... each protein sample (50 mg) was subjected to SDS/PAGE and transferred to NC membrane (Millipore, Bedford, MA, USA). Then the corresponding primary and secondary antibodies were incubated to visualize the protein ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg of proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 0.1% Tween 20 and 5% BSA for 1 h before overnight incubation with the primary antibody diluted in blocking solution ...
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bioRxiv - Immunology 2019Quote: ... chromatin was precleared by rotating for 2 h at 4°C with 100 μl of 50% protein A/G slurry (0.2 mg/ml sheared salmon sperm DNA, 0.5 mg/ml BSA, and 50% protein A/G; Sigma-Aldrich), 0.3−0.5×106 cell equivalents were saved as input ...
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bioRxiv - Biochemistry 2019Quote: Protein samples of in vitro translation reactions and subsequent purifications were separated on SDS-PAGE at neutral pH condition (pH 6.8, for purified protein samples) and were transferred on PVDF membrane (Immobilon-P, Millipore). After blocking with 5% skim milk in PBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were transferred electrophoretically to polyvinylidene difluoride membranes and probed with the following antibodies:anti-EphB6 (1:500, SAB4503476, Sigma), anti-EphB1 (1:500 ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
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bioRxiv - Neuroscience 2019Quote: ... 450-1000 µg of total protein was immunoprecipitated overnight with 20 µl (50% slurry) of anti-FLAG (Sigma, F2426) affinity gel at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the immuno cytokine was radio-iodinated with 125I (Hartmann Analytic) and Chloramine T hydrate (0.25 μg/μg protein, Sigma) and purified on a PD10 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Oligomeric α-synuclein species were removed by filtering the protein through a 50-kDa cut-off column (Amicon, Millipore). Aliquots of the protein were prepared ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then incubated with 20 μL of 50% protein G plus-agarose (Sigma-Aldrich, St. Louis, MO) for 4 h at 4°C with gentle rocking ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP dilution buffer was used to dilute the sheared chromatin followed by preclearing with Protein G Agarose beads (Sigma) for 30min ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Immunology 2019Quote: ... the proteins samples were incubated with 50 μg/mL of polymyxin B sulphate salt (Sigma-Aldrich, St. Louis, MO) for 30 min at 37 °C to remove possible residual LPS.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in a solution containing 1 % SDC and 10 mM TCEP with 40 mM CAA (Sigma-Aldrich) as reduction and alkylating agents and heated to 37 °C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Immunology 2020Quote: ... Proteins were precipitated overnight at −20°C with six volumes of pre-chilled (−20°C) acetone (Sigma-Aldrich, USA). The samples were centrifuged at 8,000xg for 10 minutes at 4°C to obtain the protein pellets and supernatants were discarded ...
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bioRxiv - Physiology 2020Quote: ... Proteins were resolved on a 4%–12% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-FL, Millipore), incubated with indicated antibodies overnight at 4°C and subsequently quantitated with the LiCOR Odyssey instrument with Odyssey Image Studio software using 2 different infrared channels ...
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bioRxiv - Immunology 2019Quote: ... 20 µg to 50 µg of protein for each sample was mixed with 2X Laemmli sample buffer (S3401, Sigma) and heated at 50°C for 10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Eight µg of IrSPI were refolded for 30 minutes at 25°C in a final volume of 100µl containing 10µg of the disulfide isomerase protein (Sigma-Aldrich) with reduced/oxidised glutathione (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... were used at 1,000 × g and 4 °C to exchange purified protein samples to 250 mM ammonium acetate (99.99 % purity; Sigma-Aldrich), pH 8.0 as buffer surrogate ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble remnants were removed by centrifugation at 16,000 g for 5 min and 140 μg clarified protein extract was transferred to a 30 kDa MW cut-off spin filter (Amicon Ultra 0.5mL Filter, Millipore). SDS was completely replaced by repeated washing with 8 M urea ...
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bioRxiv - Immunology 2021Quote: Mice were sensitized on day 0 by intraperitoneal injection of 50 μg lyophilized BSA-free ovalbumin protein extract (Sigma) resuspended in PBS and pre-incubated with the adjuvant Imject Alum (Thermo) ...