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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg protein were incubated with DNase I (7,5 U) and 1 µg anti-Flag resin (cat# F3165, Sigma-Aldrich) at 12 rpm overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... the pIVEX 1.3 WG vector suitable for cell-free synthesis of the protein with the Rapid Translation System (5PRIME, Hamburg, Germany) and (ii) the bacterial expression vector pET-30a (Novagen). The latter yielded a chimeric TatB construct carrying a combined His-/S-peptide-tag ending with a BrCN cleavage site immediately upstream of the N-terminus of mature TatB (Fig ...
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bioRxiv - Cancer Biology 2019Quote: ... Then 10 μg of nuclear protein extracts were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore). After blocking with QuickBlockTM Blocking Buffer for Western Blot (Beyotime) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were transferred to a PVDF membrane followed by incubation with Anti-Cas9 antibody conjugated to FITC (Sigma Aldrich). Anti-mouse secondary antibody conjugated to Alkaline phosphatase was used to develop the blot with NBT-BCIP (nitro-blue tetrazolium and 5-bromo-4-chloro-3’-indolyphosphate).
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred onto polyvinylidene fluoride membrane (PVDF, Hybond-P, Amersham) in transfer buffer containing 12 mM Tris (Sigma), 96 mM glycine (BDH ...
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bioRxiv - Biochemistry 2019Quote: ... and absorbance scans were performed on a spectrophotometer (Shimadzu) to identify a region of spectral overlap in absorbance maxima between the proteins and Rose Bengal dye (# 330000, Sigma). The most robust overlap occurred between 550-580 nm (Fig ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were separated by SDS-PAGE (10% acrylamide gel) and transferred to a nitrocellulose transfer membrane (Immunobilon – EMD Millipore) using a semi-dry transfer set up ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2019Quote: All tau variants (full length protein and fragment encoding amino acids 168-368 or amino acids 256-368) were cloned into the pET19b vector (Novagen) in between the NcoI and BamHI restriction sites ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We took 25 μL of standard BSA for standard solution preparation and 25 μL damselfly lysates for sample protein quantification and then added 0.1 M NaOH (Sigma-Aldrich) to make the final volume 200 μL ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were treated as previously reported to purify the target proteins by immunoaffinity chromatography using anti-HLA-DR-FF-sepharose or M2 anti flag (Sigma)(Álvaro-Benito et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Viable one-cell embryos were microinjected into the pronucleus with commercially available Cas9 protein (30ng/ul; PNABio) and guide RNAs (sgRNA; 25ng/ul; Sigma). All those components were previously hybridized in solution to generate ribonucleoprotein complexes ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein extracts (40 μg) were separated by electrophoresis on 10% SDS polyacrylamide gel and transferred onto polyvinylidene difluoride membranes (Millipore). Membranes were blocked in 5% nonfat milk in TN buffer (50 mmol/L Tris-HCl pH7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... For total lysate, proteins were directly solubilized in 4X loading buffer [(250 mM Tris, 40% glycerol, 0.008% bromophenol blue (all Sigma-Aldrich)] ...
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Microbiology 2020Quote: ... Equal protein concentrations were loaded onto 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore Corporation, Billerica, Mass.). Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... A volume of 12.5 μl of a solution containing 500 ng protein was diluted in H2O with 5× Sypro Orange (Sigma-Aldrich) and immediately mixed with an equal volume of assay condition ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Biochemistry 2019Quote: ... The T1r2a/T1r3-LBD protein was purified from the culture medium by the use of ANTI-FLAG M2 Affinity Gel (SIGMA). The purified protein was dialyzed against the assay buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: Total protein was extracted from L4/5 DRGs or retinas using the RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Microbiology 2019Quote: Bacterial cells were removed by centrifugation and culture filtrate protein (CFP) was prepared by filtering the supernatant through 0.2 µM pore size filters (Millipore, USA). After sterility testing of CFP in Mycobacteria Growth Indicator Tube (MGIT ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Microbiology 2019Quote: ... Western immunoblotting was performed by incubating the membrane with Streptavidin HRP-linked protein in PBS-BSA buffer and developing with TMB (Sigma).
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bioRxiv - Cancer Biology 2020Quote: CAF CMed was also separated into the metabolite (less than 3kDa) and protein (more than 3kDa) fractions using 3kDa centrifugal filters (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were diluted to 1 M urea with 50 mM TEAB buffer and digested with 1:100 trypsin (Sigma) 2 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 mg of lysate proteins were incubated with 40 μl of pre-washed anti-FLAG M2 magnetic beads (Sigma– Aldrich) for 2 h ...
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bioRxiv - Physiology 2019Quote: ... cells were washed twice with ice cold D-PBS and proteins harvested using RIPA buffer supplemented with cOmplete protease and PhosStop inhibitors (Sigma). The lysates were cleared by centrifugation at 13 000 rpm for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were clarified by centrifugation before GFP-FLAG-tagged proteins were isolated by incubation with 100 µL packed FLAG M2 affinity resin (Sigma) for 1 h with rotation at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were washed extensively in lysis buffer and bound proteins eluted in 500 µL 100 µg/ml 3X FLAG peptide (Sigma) in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and the concentration of the protein were done by using amicons® (Merck Millipore Ltd, cat:UFC900324).
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated to 500 μL using a 50 kDa cutoff Amicon Ultra-4 Centrifugal filter (Millipore UFC805008) and injected onto a Superdex 200 increase gel filtration column in sizing buffer (25 mM HEPES pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... A total of 105 cells or 20 mg protein was resolved by SDS-PAGE and transferred to Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk in TBS-T (25 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of protein were loaded and separated in 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membranes (Millipore). Membranes were blocked for 1 hour at room temperature with Intercept (TBS ...
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bioRxiv - Genetics 2020Quote: ... Proteins were concentrated using an Amicon Ultra-0.5 mL centrifugal filter device with a cutoff of 100 kDa (Millipore Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The protein was then concentrated by centrifuging at 5000 RPM in an Amicon® Ultra-15 10K centrifugation column (Millipore). Protein concentration was measured by UV absorbance at 280nm using an Ultraspec 2100 pro by Amersham Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed from the labeled proteins by successive concentrations and dilutions using a 3 K MWCO centrifugal concentrator (Amicon Ultra-4, Millipore). The labeling efficiency was computed by measuring the concentration of the protein and Cy3 dye using their absorbances at 280 nm and 550 nm ...
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bioRxiv - Immunology 2021Quote: ... the semi-dry protein pellet was dissolved in 25µL of 8 M urea in 20mM EPPS (pH 8.5) (Sigma; Cat#E9502) and was then diluted with EPPS buffer to reduce urea concentration to 4 M ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Biochemistry 2021Quote: ... 1mL (2 mg) of protein was added to 20 µL of ANTI-FLAG® M1 Agarose affinity Gel (Sigma Aldrich) pre-equilibrated in TBS and incubated on a nutator for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Immunology 2021Quote: Approximately 5×105 cells stably expressing the indicated TurboID-V5-fusion proteins were treated as described prior to the addition of 500μM biotin (Sigma-Aldrich) for 30min ...
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bioRxiv - Bioengineering 2020Quote: ... For western blotting 40 μg of protein samples were subjected to 10% in house SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 3% BSA in TBS containing 0.01 % Tween-20 for 1 h at room temperature and then incubated with specific primary antibodies at 4 °C overnight in the blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: To expose Prdx2 to continuous H2O2 generation the protein (20 μM) was incubated with 5 mM glucose plus glucose oxidase (Sigma). Glucose oxidase was diluted to produce approximately 0.5 μM H2O2/min.