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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Blast colony forming cell (Bl-CFC) assays were performed using iPSC cell pellets resuspended in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma-Aldrich S0192) supplemented with 1% penistreptomycin and L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... IgG3 and IgM) in mice were determined after infection with MORV and VSV using mouse Isotyping multiplex assay (MGAMMAG-300K, Millipore Sigma, USA) by Luminex xMAP technology as recommended by the manufacture.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... magnetic force was applied by placing the assay plates on a handheld magnetic separation block (V&P Scientific 771HH-H/Millipore 40-285). In all subsequent experiments ...
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bioRxiv - Biochemistry 2024Quote: ... Then 20 µl of the mix was transferred to a tube containing 10 µl 3x Stop buffer (assay buffer + 100 mM EDTA, 500 µM proteinase K (Sigma-Aldrich, 107393), 1% SDS ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in samples was determined using Bradford assay (Bradford, 1976) against standards of known protein concentration prepared using bovine serum albumin (BSA; Sigma-Aldrich, UK).
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bioRxiv - Molecular Biology 2024Quote: The standard assay for ALP activity was carried out in 500 μL of the reaction mixture containing 2 mM pNPP (Sigma-Aldrich, USA) in 0.1 M Tris–HCl buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Neuroscience 2023Quote: ... the entire frozen neocortex of the left hemisphere was grinded by Dounce homogenizer in RIPA buffer (radioimmunoprecipitation assay buffer, 20-188, Millipore, CA, USA) in the presence of protease inhibitors and phosphatase (Roche ...
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bioRxiv - Genetics 2023Quote: ... The IC90 at day 6 of treatment for each drug was determined by treating the inducible SpCas9 single clone (before gRNA library infection) with a dose curve of each drug in triplicate and taking an MTT assay measurement (Sigma Aldrich 11465007001) on day 6 following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 90 μL of the remaining solution after ALP assay was mixed with 90 μL of digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).
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bioRxiv - Physiology 2023Quote: ... containing 12.5µL of assay buffer (125mM KCl, 20mM HEPES, 2mM MgCl2, 2mM KH2PO4, pH =7.4) supplemented with 40mM succinate (Sigma-Aldrich # S3674-100G), 40mM malate (Sigma-Aldrich #240176-50G) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then treated in triplicates for 24hrs with varying concentrations of rhIL-23 for analysis via a WST-1 proliferation assay using WST-1 reagent (0501594400, Sigma-Aldrich, Canada) and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091 ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in XF DMEM media immediately prior to experiments: Oligomycin 5μM (Sigma Aldrich cat. 495455), Rotenone 2.5μM (Sigma Aldrich cat ...
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bioRxiv - Systems Biology 2024Quote: ... or thawed on ice from frozen preparations and 25 μg from each sample was prepared for glutamate dehydrogenase assay according to manufacturer instructions (Millipore Sigma MAK099). The absorbance at 450 nm was then measured every two minutes for 4 hours on Cytation 5 (Biotek ...
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bioRxiv - Molecular Biology 2024Quote: ... were transferred to a 96-well plate and overlayed with 120 µL of assay buffer containing DTNB (D8130, Sigma; 2 mg/ 3mL) and 10 U glutathione reductase (G3664 ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cancer Biology 2022Quote: ... EV were labeled with a PKH67 dye labeling kit-green (or PKH26 dye labeling kit-red) following the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate or 15N-ammonium labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Microbiology 2021Quote: ... Alginate lyase was removed from culture medium prior to assay by filtering the medium through 3 kDa Amicon Ultra molecular weight cutoff filters (MilliPore Sigma, Burlington, MA). To maintain plasmids in 12B01,12.5 µg/mL chloramphenicol was added to the medium.
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill, UK) and Anatrace (Ohio ...
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bioRxiv - Biophysics 2019Quote: ... Purity was examined by SDS-PAGE and concentration determined by Bradford assay before loading onto a 20 mL spin column packed with 5 mL ssDNA-cellulose (Sigma Aldrich D8273-5G). The column with SSB containing fractions was sealed and incubated for 60 min with gentle rocking ...
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bioRxiv - Biochemistry 2020Quote: ... The GnT I assay was subsequently performed in GnT I activity buffer [63] containing 5 mM of UDP-GlcNAc (Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Microbiology 2020Quote: The MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex – Immunology Multiplex Assay (Millipore, Burlington, MA, USA; #PCYTMG-40K-PX23) was performed on collected plasma samples following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop, 4906837001, Sigma-Aldrich, USA) The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Samples obtained from in vitro proteolysis assay were analyzed by electrophoresis on an 8% SDS-polyacrylamide gel followed by transfer to Immobilon polyvinylidene difluoride membranes (Millipore Co., Bedford, MA). The membranes were blocked using 4% non-fat milk and immunostained with specific mAb ...
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2021Quote: ... ALT was measured in 1 microliter of plasma collected via cardiac puncture or tail vein bleed using a colorimetric assay (Millipore Sigma cat. # MAK052) according to the manufacturer’s instructions.
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Neutralization assays were performed by pre-incubating cells with anti-GalactosylCeramide antibody (mGalC, 10µg/mL, Millipore 10µg/mL, 30 min at 4°C) or viral inoculum with monoclonal antibody against HIV-1 gp120 (1-2µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of siderophores produced by AMB-1 was performed using the Chrome Azurol S (CAS) assay (199532, Sigma-Aldrich, St. Louis, Missouri, USA) [47] ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the workflow of this assay involves four main steps: (i) Tissue is harvested and lysed in lysis buffer containing S-Methyl Methanethiosulfonate (MMTS, Sigma, Cat no. 64306) to block free thiol groups (-SH) ...
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bioRxiv - Physiology 2022Quote: Tissue triglyceride levels were measured using an enzymatic triglyceride assay (Glycerol Reagent, Cat# F6428, Triglyceride Reagent, Cat#T2449, Sigma-Aldrich, Oakville, ON, Canada). In a flat bottom 96 well plate ...
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bioRxiv - Physiology 2024Quote: ... [NH3 + NH +]) was determined spectrophotometrically in 25 µL aliquots of flash frozen plasma by enzymatic ammonia assay (Sigma-Aldrich, USA, Catalog number: AA0100). [TAmm] was calculated from the delta absorbance at 340 nm wavelength before and after the addition of the enzyme L-glutamate dehydrogenase ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Microbiology 2023Quote: Proteins were isolated from hPCLS with the radioimmunoprecipitation assay (RIPA) buffer (Thermo, Cat.# 89901) containing a cocktail of protease inhibitors (Sigma, Cat.# P8340-5ML) and phosphatase inhibitors (Fisher ...
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bioRxiv - Genetics 2023Quote: ... exact number recorded per vial for assay read-out) were then exposed to standard medium prepared with 200mM Mifepristone (RU486) from Sigma Aldrich (cat# 856177) and 2.5% w/v FD&C Blue Dye no 1 from Spectrum (cat# FD110 ...