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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... assays22 and nanostructure-initiator mass spectrometry (NIMS)23 assays were performed on the supernatant fraction and the remaining material was filtered using Miracloth (Millipore Sigma, Burlington, MA, USA) and dried at 50 °C to determine the biomass dry weight.
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Pathology 2019Quote: Plasma samples from cohort A rhesus macaques were analyzed using Milliplex map Nonhuman Primate Cytokine Magnetic Bead Panel Premixed 23-Plex Assay (EMD Millipore Corporation, Billerica, MA). Assays were run on a Bio-Plex 200 system and analyzed using Bio-Plex Manager Software version 6.1.1 (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Physiology 2020Quote: ... and 120 minutes thereafter and blood glucose concentrations were measured by colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO).
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO) and tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed or tissue mechanically homogenised in lysis buffer (Extraction buffer, FNN0011) and protein content was quantified by a BCA assay (Sigma-Aldrich, St. Louis, MO). Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... leaf discs were placed in a 96-well plate containing 50 μL of distilled water and 50 μL of assay solution containing 20 mM Luminol (Sigma-Aldrich, St. Louis, A8511), and 1 mg/mL peroxidase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... total mRNA was measured using the RiboGreen assay with an upfront lysis step in 2% Triton X-100 (SX100-500ML; Sigma Aldrich, St. Louis, MO) to lyse the LNPs ...
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bioRxiv - Physiology 2022Quote: The subcellular distribution and possible spatial association between S-NO and Cx43 were evaluated by Proximity Ligation Assay(Soderberg et al., 2006) (Sigma, St. Louis, MO, USA). Tissue sections (6 μm ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay (EMD Millipore Corporation, Billerica, MA, USA). and custom FAS panel on the Luminex MAGPIX ...
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bioRxiv - Neuroscience 2022Quote: Brain punches were homogenized in radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase inhibitor cocktail 1 and 2 and protease inhibitor cocktail (Sigma Aldrich, Saint Louis, MO) followed by centrifugation at 16,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Inflammatory proteins were measured using the MILLIPLEX Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex – Immunology Multiplex Assay (EMD Millipore. Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS at room temperature (RT) and lysed in 1x (radio immunoprecipitation assay buffer (RIPA buffer, Merck Millipore, cat. no. 20-188) containing 0.1% SDS ...
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bioRxiv - Cancer Biology 2024Quote: ... Panel balance and individual [Nuc:bead] identity were confirmed in Luminex assays with anti-dsDNA (EMD Millipore #MAB030; 1/5, 1/50 and 1/500), anti-H3.1/2 (Active Motif #61629 ...
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bioRxiv - Immunology 2023Quote: ... Thirty-two markers were simultaneously measured in the samples using Eve Technologies’ Mouse Cytokine 32-Plex Discovery Assay® (Millipore Sigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: DNA indexes with different overhang sequences were validated by performing in-situ ligation assays on 4T1 mouse cells grown on 25mm round coverslips coated in poly-L-lysine overnight (Sigma P4707, 0.01% w/v). Cells were grown for 24-48hrs to ∼90% confluence.
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bioRxiv - Biochemistry 2024Quote: ... In our studies the experimental rapid Mg2+ influx was normalized to the control (Eq 1): In some assays a final concentration of 1 mM Co(III)Hex (Sigma Aldrich, # 50-180-0331) was added to inhibit Mg2+ import but not export ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions: anti-human γH2AX Ser139 (EMD Millipore; JBW301; 1:200), goat anti-mouse IgG AF546 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Primary cultures fixed and permeabilised as described for IF were used to perform PLA using a commercial kit (Duolink® PLA starter kit, Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA of control and insertion lines was extracted using a commercial kit (Spectrum™ Plant Total RNA Kit, Sigma life science) following the supplier’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were routinely tested negative for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or LookOut Mycoplasma Detection Kit (Sigma-Aldrich, MP0035). The INF2-KO U2OS cell line made by CRISPR-Cas9 is described elsewhere (Chakrabarti et al. ...
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bioRxiv - Physiology 2021Quote: We used ELISA kits to detect the concentrations of inflammatory mediators such as TNF-α (KIT ELISA TNF-a-RAB0477-1KT, Sigma-Aldrich) and IL-6 (KIT ELISA IL-6-RAB0308-1KT ...
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bioRxiv - Molecular Biology 2023Quote: AKT:FBP1:ALDOB:PP2A-C interactions in liver tissue were detected with an in situ PLA kit (Duolink® In Situ Red Starter Kit Mouse/Rabbit, Sigma, DUO92101) (Su et al. ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted using Qiagen RNeasy Mini Kit (Maryland, USA) according to the manufacturer’s protocol and DNase-treated using the DNase I kit (Millipore Sigma, Massachusetts, USA). The quantity and quality of RNA were determined using the Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) and DNeasy® Plant Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and Spectrum Plant Total RNA Kit (Ref. STRN50, Sigma), respectively ...
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bioRxiv - Molecular Biology 2022Quote: The tartrate-resistant acid phosphatase (TRAP) kit (Sigma-Aldrich) was used for osteoclasts detection on paraffin sections of twelve weeks old hind paws using the TRAP kit following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: In Situ Cell Death Detection Kit (11684795910, Sigma-aldrich) was used to evaluate cell death in monolayer cultured neural progenitor cells according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: PLA was performed using the Duolink kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: RNA was extracted by RNA miniprep kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µl of L-Glutamate Dehydrogenase (Ammonia kit, Sigma), 2 µl of 150 mM NAD+ ...
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Cholesterol concentration was estimated using a kit from Sigma/Aldrich (MAK043) ...
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bioRxiv - Molecular Biology 2019Quote: ... and GenElute™ mRNA Miniprep kit (MRN10, SIGMA-ALDRICH), respectively.
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bioRxiv - Cell Biology 2020Quote: ... 50 μl Cell Counting Kit – 8 solution (Sigma Aldrich) was added to each well and cells were incubated for 1 h in the cell culture incubator and absorptions at 450 nm was measured.
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bioRxiv - Genetics 2021Quote: ... We used Spectrum Plant Total RNA Kits (Sigma Aldrich) for RNA extraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the Periodic Acid-Schiff staining kit (Millipore Sigma, 101646) procedure was followed as described by manufacturer ...
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bioRxiv - Biophysics 2021Quote: ... The Duolink In Situ Proximity Ligation kit (Sigma; DUO92007) was used as per the manufacturer’s instructions with fluorophore-conjugated secondary antibody to recognize HA (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... We used the Masson’s Trichrome Stain Kit (Sigma Aldrich) following manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML ...
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bioRxiv - Neuroscience 2021Quote: ... or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma, MAK266).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were measured using a commercial test kit (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the blotted membranes were visualized with chemiluminescent kits (Millipore). Densitometric analyses were performed using ImageJ software.
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bioRxiv - Plant Biology 2020Quote: ... all chemicals and kits were obtained from Sigma-Aldrich Inc.
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bioRxiv - Microbiology 2020Quote: ... using the high capacity RNA to cDNA kit (Sigma) following manufacturer guidelines ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Yeast genomic DNA was isolated with the SDS/LiAc protocol [29] ...
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bioRxiv - Plant Biology 2021Quote: ... Labeling was detected using the Immobilon western kit (Millipore) or the SuperSignal West Femto Maximum sensitivity substrate (Thermo Fisher Scientific) ...