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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Cell Biology 2019Quote: ... treated (5 Gy) fixed (1h post IR) with formaldehyde (3.7% in PBS) and the PLA assays (Duolink PLA technology, Sigma-Aldrich, DUO92014) performed using manufacturer’s protocol with antibody staining performed as in the standard immunofluorescence procedure with antibodies against V5 tag and Treacle or NBS1 and Treacle
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bioRxiv - Microbiology 2021Quote: ... permeabilized with 0.2% Triton X-100 (in Phosphate Buffered Saline [PBS]) and subjected to an immunofluorescence assay (IFA) using a monoclonal antibody (MAb) anti-ACE2 (Sigma-Aldrich), and then incubated with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2020Quote: Alkaline phosphatase activity (APA) was evaluated through fluorometric assay following the hydrolysis of the substrate (S) 4-methylumbelliferyl phosphate (MUF-P, Sigma-Aldrich) to 4-methylumbelliferyl (MUF) ...
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bioRxiv - Microbiology 2020Quote: ... For the phenotypic and proteomic assays after treatment with hydrogen peroxide a final concentration of 5 mM and 10 mM were selected (Sigma-Aldrich). For susceptibility assays C ...
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bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells grown in hypoxic chambers were frozen at the time points mentioned in the results section and harvested all together by lysing in Radioimmunoprecipitation assay buffer (RIPA buffer) containing 1× protease inhibitor cocktail (Sigma-Aldrich). For each sample ...
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bioRxiv - Immunology 2022Quote: ... Cell number and viability were examined each day in triplicate wells using trypan blue exclusion and cell proliferative assay (# Cat. no. T8154, Sigma-Aldrich). The assay was performed for 10 d post-seeding i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... pulverized in liquid nitrogen and protein was extracted using a 1x radioimmunoprecipitation assay buffer (50mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Nonidet®40 Substitute (74385; Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified using a chloramine-T assay (Huszar et al., 1980) with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Cell Biology 2022Quote: ... The following small molecules required for the MTS assay were ordered individually and 10 mM stocks prepared and stored at −20°C: (CCCP, Sigma, C2920), (Oligomycin ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Physiology 2022Quote: ... and 120 minutes following the injection using a colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO). Area under the curve measurements were calculated using the trapezoidal method (37).
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bioRxiv - Plant Biology 2022Quote: ... Quantitative measurements were performed with an enzymatic assay using myrosinase/thioglucosidase from Sinapis alba (Sigma-Aldrich CAS-No. 9025-38-1) and a D-Glucose Assay Kit (glucose oxidase/peroxidase ...
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bioRxiv - Physiology 2022Quote: ... Samples were then incubated for 15 min at room temperature with assay buffer containing glucose oxidase (0.2 U/mL, Sigma-Aldrich G7141), horseradish peroxidase (0.3 U/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Concentrations of RMAs in the collected blood were determined using the standard curves generated by the luciferase assays conducted on the normal mouse serum (Sigma-Aldrich) added with RMAs at known concentrations ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified in scaffolds using a chloramine-T assay [35] with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Bioengineering 2022Quote: ... content of scaffolds was measured using a dimethyl methylene blue (DMMB) assay [36] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Cell Biology 2022Quote: ... and homogenized using a Potter-Elvehjem tissue grinder on ice in ice-cold incomplete radioimmunoprecipitation assay buffer (RIPA) containing 50 mM Tris-HCl pH 7.4 (Sigma-Aldrich, T1503) and 100 mM NaCl (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: We quantified the T7 phage titers extracted from the washed and unwashed pipette tips using the standard double-layer plaque assay (T7Select® System Manual, Novagen). A single colony of BL21 bacteria was grown in 4 ml M9LB agar media overnight at 37°C while shaking at 250 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... glutathione levels in biological samples were measured using Ellman’s Reagent and glutathione reductase using Glutathione GSH/GSSG Assay (Sigma-Aldrich, #MAK440) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Neuroscience 2023Quote: ... buffer (for Sanger sequencing and T7 assays) or 1X sodium borate (SB) buffer (for genotyping) with 1.5 mg/mL ethidium bromide (Sigma-Aldrich®). Samples were run alongside 5 μL of 1kb Plus DNA Ladder (New England Biolabs® ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa cytotoxicity on A549 cells was assayed using a colorimetric assay that detects the number of metabolically active eukaryotic cells able to cleave the MTT tetrazolium salt (Sigma-Aldrich) to the insoluble formazan dye ...
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bioRxiv - Synthetic Biology 2022Quote: ... The buffer of the combined flowthrough was exchanged into assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) using a G25 (Sigma-Aldrich) column ...
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bioRxiv - Physiology 2023Quote: ... The resulting crude mitochondrial pellet fraction was resuspended in MIB and protein concentration was quantified with Bradford assay (Sigma-Aldrich, B6916) using a spectrophotometer (Infinite 200 Pro ...
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bioRxiv - Biochemistry 2023Quote: Reporter silencing assays were conducted as in ref 12 with the following changes: cells were treated with 1ug ml−1 dox (Sigma-Aldrich), and gates were set based on reference reporter cells cultured in parallel without dox and reference fully silenced reporter cells (See gating scheme in Figure S2).
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bioRxiv - Molecular Biology 2023Quote: Harvested cells were lysed in radioimmunoprecipitation assay (RIPA) buffer with the addition of protease inhibitor cocktail (Sigma-Aldrich, Cat. No. P83490), briefly sonicated ...
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bioRxiv - Bioengineering 2023Quote: ... Levels of inflammation-associated cytokines were assessed by MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assay (Millipore Sigma), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Samples were raised to a final volume of 50 μL with Triglyceride Assay Buffer (CAS# MAK266, Sigma Aldrich, St. Louis, MO). 2 μL of Lipase was added to each sample and was incubated for 20 minutes at room temperature to convert triglyceride to glycerol and fatty acids with the plate shaken manually every ten minutes ...
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bioRxiv - Molecular Biology 2023Quote: LX-2 cells were lysed and proteins were extracted using radioimmunoprecipitation assay buffer supplemented with complete EDTA-free protease inhibitor cocktail (Sigma-Aldrich). After centrifugation of cell lysates at 12,000 x g for 10 min ...
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assay (PLA) was performed in MCF10A cells using the DuoLink® in situ Orange detection reagent (DUO92102, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... supernatant of cells was collected and used for quantitating levels of nitrite and nitrate using a Nitrite/Nitrate Assay (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... The final part portion of the lysate was used to quantify the collagen content according to a hydroxyproline assay (Sigma-Aldrich). The absorbance was measured at 570 nm and the collagen content in the sample was determined from a hydroxyproline standard.
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bioRxiv - Immunology 2023Quote: ... Infected and mock controls were washed three times in PBS and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Millipore 20-188) at 2 x with protease inhibitor for western blot analysis.
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bioRxiv - Cell Biology 2022Quote: ... medium was changed to pre-mixed treatments in lipolysis assay medium: insulin 100 nM (Humulin-R U-100, Lilly) and/or isoproterenol 1 mM (Sigma-Aldrich). Lipolysis was measured over 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was spun down at 16,873 x g for 10 min and supernatant was diluted to 2 mg/ml after quantification by Bradford assay (Sigma Aldrich). The sample was reduced for 30 min by adding DTT to a concentration of 5 mM followed by an alkylation step with acrylamide at 15 mM for 30 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... For phage dilutions in plaque assays we used an SM buffer supplemented with 10 mM calcium chloride and magnesium sulphate (Sigma-Aldrich). We routinely stored phages as filter-sterilised (0.22 μm ...
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bioRxiv - Physiology 2023Quote: ... Samples of this amniotic fluid supernatant were submitted to the ONPRC/OHSU Endocrine Technologies Core (ETC) to set up bead-based multiplex assays to analyze multiple cytokine and chemokine biomarkers using the Luminex technology (Millipore Sigma).
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bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in MA media immediately prior to experiments (FCCP 1μM; Sigma Aldrich cat. C2920).
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial lysates for the assay were prepared via dilution of the final isolated mitochondrial suspensions in CelLytic M (Sigma-Aldrich; C2978) at a protein concentration of 2mg/ml ...