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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Two New Zealand White rabbits were injected at ten sites with 500 µl of Freund’s Complete Adjuvant (Sigma-Aldrich F5881) containing 500 µg of Tin protein ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich), α-ZC3HAV1 (GTX120134 ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich) or with isotype control (12-370 ...
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bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-β-actin (A5441, clone AC-15) and rabbit polyclonal anti-Calnexin (#C4731) were obtained from Sigma-Aldrich. Mouse monoclonal anti-V5 (BLD-680605 ...
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bioRxiv - Cell Biology 2023Quote: Polyclonal antibody against the C-terminus of mouse SETX-C were generated by immunizing rabbits. Mouse cDNA encoding Setx (aa. 1900-2701) was subcloned into pET15b (Novagen) and transformed to E.coli strain BL21-CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The wells were washed as before and then probed with 100µl rabbit anti-chicken IgG conjugated to horseradish peroxidase (HRP) (Sigma Ltd.) diluted 1:3000 in ELISA diluent for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The precleared supernatant was divided into two Eppendorf tubes and beads were either cross-linked to the bait antibody (Grin2b-rabbit #06-600 Millipore,Ppp1ca-Abcam Rabbit #ab16446 ...
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bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Neuroscience 2024Quote: ... This was followed by incubation for 90min at room temperature (RT) with secondary antibody anti-goat or anti-rabbit IgG Biotin (Sigma) diluted 1:400 or 1:300 ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was probed with the indicated primary antibody (rabbit anti-GFP [1:1500, Abcam, #ab290], mouse anti-RFP [1:1000, Chromotek, #6G6], and mouse anti-2A [1:1000, Sigma, #3H4]), washed with TBST ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit polyclonal anti-FLAG (F7425, 1:200), mouse monoclonal anti-vinculin (V4505, 1:200), and mouse monoclonal α-SMA (A5228, 1:200) from Sigma; rabbit polyclonal anti–SOX9 (#82630 ...
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bioRxiv - Neuroscience 2021Quote: ... cross- and longitudinal sections of the sciatic nerve were stained with Tuj-1 (1:500, sigma, T5076) and ChAT (1:50, Millipore, AB144P). The numbers of Tuj-1-positive axons ...
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bioRxiv - Neuroscience 2021Quote: ... chicken anti-GFAP (Chemicon, AB5541, 1:3000, RRID:AB_177521), mouse anti-SMI-32 (Covance, SMI-32R, 1:2000) and Hoechst 33342 (Sigma Aldrich, 1:300) for nuclear labeling ...
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bioRxiv - Molecular Biology 2020Quote: ... empty biotin modified hydrogel spheres were modified with 1:1 volume ratio of 1 mg/ml streptavidin (Sigma-Aldrich, cat #85878) for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... was obtained from the Swansea University culture collection and grown on Sabourand dextrose agar (SDA, 40 gL−1 D-glucose, 10 gL−1 mycological peptone, 5 gL−1 technical agar (Sigma,UK), 0.5 gL−1 chloramphenicol ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... A small aliquot from the final suspension was taken and diluted 1:4 in CCM and then 1:1 in Trypan Blue (T8154, Sigma-Aldrich). A live/dead cell count was determined by hemocytometer and a cell viability was assessed ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Cell culture supernatant was diluted 1:1 in IP buffer (50 mM Tris-Cl, 75 nM NaCl, 1% Igepal, protease inhibitor mixture (Sigma, 11836153001), pH 7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403 ...
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bioRxiv - Physiology 2021Quote: ... anti-TRPV4 (1:200, Alomone, #ACC-034) and anti-mCherry (1:500, NOVUS, #NBP1-96752) or anti-Flag (1:1000, Sigma, #F1804), at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... IF 1:500), mouse anti-cortactin (05-180, IF 1:400), mouse anti-talin (T3287, IF 1:200) all from Sigma-Aldrich; rabbit anti-EEA1 (ALX-210-239 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... PC12 cells were incubated in serum-reduced medium (1% horse-serum, 1% FBS) supplemented with 100 ng.ml-1 ß-NGF (Sigma-aldrich) during five days on PDL coated surface.
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bioRxiv - Biochemistry 2022Quote: Reporter cells were plated in triplicate at 1 × 105 cells ml−1 in media with or without 100 ng ml−1 doxycycline (dox) (Sigma-Aldrich). Every 3 d ...
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bioRxiv - Cell Biology 2022Quote: For vinculin staining hMSCs are fixed with ice cooled mixture of 1:1 (v/v) (4% PFA: permeabilizing buffer (1% Triton-X-100-Sigma Aldrich) for one minute on ice ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4, 1:200; α-GluA1, Cell signaling #13185, 1:150, α-myc, Sigma #M5546, 1:1000) were applied to neurons for 10 min at room temperature followed by a washing step in PBS supplemented with 0.1 mM CaCl2 and 1 mM MgCl2 and paraformaldehyde fixation.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were incubated overnight with primary antibodies (Anti-DNALI1: 1: 100, Abcam, Cat No: ab87075; Anti-PACRG: 1: 200, generated by our laboratory; Anti-α-tubulin, 1:200, Sigma, Cat No ...
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bioRxiv - Microbiology 2021Quote: ... Strains were seeded in lysogeny broth (LB, 10 g L-1 NaCl, 10 g L-1 tryptone, 5 g L-1 yeast extract, Sigma-Aldrich) at 30°C with shaking overnight ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 1 ml acetone/water (1:1, Carl Roth, Karlsruhe, Germany) and 0.1 % trifluoroacetic acid (Sigma Aldrich, Dreieich, Germany). 170-200 µL DHB matrix solution was applied to the sections using a pneumatic sprayer system with a nitrogen pressure of 0.7-0.75 bar ...
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bioRxiv - Cell Biology 2020Quote: ... then plated onto LB plates containing the appropriate antibiotic (100 μg ml−1 ampicillin, 50 μg ml−1 kanamycin or 25 μg ml−1 chloramphenicol, Sigma Aldrich). Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich) ...