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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: Evolution under antibiotic exposure started with a 1:1 ratio of overnight susceptible and resistant strains diluted 1:50 into fresh media supplemented with ampicillin (Millipore cat# 171254) and lysine (as indicated in the text) ...
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bioRxiv - Genetics 2024Quote: ... The primary antibodies (NF-kB, #8242 Cell Signaling, 1:1000; CD3, MCA-1477 Biorad, 1:100; acetylated Tubulin, T6793 Sigma, 1:1000; Slc12a3, HPA028748, Sigma Aldrich, 1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting collagen solution was then mixed in a 1:1 ratio with 1 U/mL bovine thrombin solution (Sigma, Cat. # T4648) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Media was then swapped to 200 ng ml-1 FGF9 (R&D, 273-F9-025) and 1 µg ml-1 heparin (Sigma Aldrich, H4784) in TeSR-E6 for 5 days to induce nephron progenitors ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed and incubated with appropriate secondary antibody diluted in TBS-T (HRP conjugated αMouse, 1:10000, CST, 7076S; HRP conjugated αRabbit, 1:10000, CST, 7074S; HRP conjugated αSheep, 1:5000, Sigma-Aldrich, A3415) for at least an hour before being washed and revealed (BioRad Clarity or Clarity Max ECL) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3 times with PBS and incubated with blocking solution (PBS/BSA 1%) for 1 h at 37 °C and then with the following primary antibodies: mouse anti-GFAP (1:500; Millipore, Cat. MAB360), rabbit anti-C3 (1:25 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with goat serum diluted 1:200 in PBS for one hour followed by an overnight incubation at 4°C with the primary antibody (HIF-1α, 1:200, described in32; BACH-1, 1:200, Sigma Aldrich HPA034949) Next ...
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bioRxiv - Biophysics 2024Quote: ... Panel balance and [Nucleosome : bead region] identity were confirmed using anti-dsDNA (EMD Millipore #MAB030; 1/5, 1/50 and 1/500), anti-H3.1/2 (Active Motif #61629 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Alexa-488 goat anti-mouse and Alexa-555 goat anti-α-rabbit (Sigma-Aldrich, St. Louis, MO) (diluted 1:150 in PMEBALG ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were blocked for at least 2.5 hours at room temperature followed by the addition of poly-clonal rabbit anti-histone H4 (citrulline 3) antibody (EMD Millipore) used at 1:100 and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10000 for 1 hour (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for immunoprecipitation: HSF1 (ADI-SPA-901, Enzo), HSF2 (Östling et al., 2007) and normal rabbit IgG (EMD Millipore). Crosslinks were reversed by incubating the samples at 65°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then labeled with the PLA antimouse PLUS probe (DUO92001) and the PLA anti-rabbit MINUS probe (DUO92005) purchased from Sigma-Aldrich. Detection was performed with Duolink in situ detection reagents red kit (DUO92008 ...
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bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449 ...
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bioRxiv - Microbiology 2020Quote: ... measuring the decrease in absorbance at 340 nm 30 min after addition of 5 U of L-lactic dehydrogenase (from rabbit muscle, Sigma-Aldrich) and 4 U of pyruvate kinase (from rabbit muscle ...
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bioRxiv - Molecular Biology 2021Quote: ... PLA was performed according to the manufacturer’s instructions using the following kits and reagents: Duolink in-situ PLA Probe Anti-Rabbit PLUS (Sigma-Aldrich # DUO92002), Duolink in-situ PLA Probe Anti-Mouse MINUS (Sigma-Aldrich # DUO92004) ...
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bioRxiv - Microbiology 2019Quote: ... PltB-3xFLAG and ArtB-c-Myc were detected in sub-fractions using western blot analyses performed with rabbit anti-FLAG (Sigma-Aldrich) and rabbit anti-c-Myc (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... and slices were incubated overnight at 4 °C in a PBST-based antibody carrier solution containing 3% normal goat serum and rabbit anti-TPH2 primary antibodies (Millipore, ABN60) diluted 1:1000 ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The RBM proteins were examined with the primary antibodies and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Immunology 2019Quote: ... The microarray was then rinsed for 3 times with washing buffer and incubated with 200 μL of Horseradish peroxidase (HRP) conjugated Rabbit anti-Goat IgG (Sigma-Aldrich) diluted 1:20000 in Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples of PSD95 and Kal7 or PSD and Kal8 were incubated at 4oC overnight with rabbit anti-Flag Antibody (#F7425 Sigma; RRID:AB_439687) in a final volume of 120μl 20mM TES ...
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bioRxiv - Neuroscience 2020Quote: ... The specimens were incubated at room temperature for 1 h in blocking buffer (PBSTX with 10% normal sheep serum and 2% bovine serum albumin) and subsequently with the primary antibody (rabbit-anti-GABA, Sigma-Aldrich A2052 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times in PB and incubated for 24 hours at 4°C under continuous stirring with rabbit primary polyclonal antibody against Cy3-conjugated NeuN (Millipore, ABN78C3) diluted 1:1000 in PB ...
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bioRxiv - Cell Biology 2021Quote: ... proximity ligation assays were performed on isolated HSCs with the Duolink in Situ Red Starter Kit Mouse/Rabbit (DUO92101, Millipore Sigma), adapting the vendor’s protocol for HSCs ...
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bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Cell Biology 2021Quote: ... we used rabbit polyclonal anti-AFG3L1 (Koppen et al, 2007) (Figure 1A, B and Figure S1C) and rabbit polyclonal anti-FLAG (#F7425 from Sigma-Aldrich) (Figure 3 ...
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bioRxiv - Physiology 2021Quote: ... Protein detection was performed by the use of the HRP-conjugated secondary antibodies: anti-rabbit (CST #7074) or anti-mouse (CST #7076) and Immobilon Forte Western HRP substrate (Millipore Sigma). Protein band intensity on exposed film was measured with Adobe Photoshop software.
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bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated for 90 min with anti-rabbit IgG antibody conjugated to alkaline phosphatase (Sigma Alrich), diluted 1:5.000 in TBS-T solution containing 0.75 % BSA ...
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bioRxiv - Biochemistry 2020Quote: ... Chemiluminescence of the appropriate secondary rabbit or mouse HRP-conjugated antibodies after incubation with ECL HRP substrate (Immobilon™ Western HRP Substrate, Millipore) was detected using a Chemi Doc™ Quantity One® system (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Microbiology 2020Quote: ... were used with secondary anti-mouse IgG or anti-rabbit IgG conjugated with horseradish peroxidase antibodies (A9917 and AP132P, Sigma Aldrich). SuperSignal West Pico Chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Mouse phospho-MLKL (Ser345) rabbit monoclonal (D6E3G) (Cell Signalling Technology) MLKL rat monoclonal 3H1 (available from Millipore MABC604; generated in-house) ß-actin mouse monoclonal AC-15 (Sigma A-1978)
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 2% paraformaldehyde in PBS and proximity ligation assays (PLA) were performed with the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... of SPTAN1-kRas and SPTAN1-Raf1 were identified using the Duolink® proximity ligation assay (PLA) in situ red for mouse/rabbit antibodies (DUO92101, Sigma). THP-1 cells were fixed onto microscope slides and permeabilized as outlined in the ICC/IF section ...
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bioRxiv - Immunology 2022Quote: ... Slides were then washed in dH2O and TBS-T before incubation overnight at room temperature with a rabbit anti-CD34 antibody (HPA036723, Millipore Sigma), a mouse anti-CD20 antibody (M0755 ...
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bioRxiv - Biochemistry 2022Quote: ... and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804; rabbit anti-NF-145 – Millipore AB1987), followed by washing in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins transferred to nitrocellulose membranes were probed with either affinity-purified polyclonal rabbit anti-Gli326 or goat polyclonal anti-Gli3 (R&D, AF3690) and mouse anti-vinculin (Sigma, V9264) and visualized with fluorescent secondary antibodies (LI-COR IRDye 800CW ...
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bioRxiv - Neuroscience 2020Quote: ... Guinea pig anti-vesicular glutamate transporter 2 (VGluT2) (Cat#2251-I; RRID: AB_2665454) and Rabbit anti-NeuN (Cat# MAB377; RRID: AB_2298772) was purchased from Millipore (Temecula, CA, USA). Rabbit anti-PAX6 (Cat#901301 ...
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bioRxiv - Immunology 2020Quote: ... the primary antibodies were discarded and the sections probed with secondary antibody (anti-rabbit IgG conjugated to FITC) (Sigma-Aldrich, Germany) prepared at a dilution of 1:10000 in PBST ...
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bioRxiv - Microbiology 2020Quote: ... The heat inactivated sera were serially diluted in BSK-II media without rabbit sera followed by being mixed with complement-preserved sera from guinea pig (Sigma-Aldrich) or chicken (Biowest ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The following day plates were washed three times with TBS-T and incubated for 2 hours at room temperature with secondary antibody horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917), diluted to 1 in 1,000 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: Blots were washed three times for 15 minutes with PBS-T and probed 45 minutes with secondary antibodies (rabbit anti-mouse (Sigma A4312), 1:10000 dilution ...