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Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
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bioRxiv - Physiology 2019Quote: ... fiber type IIb) (hybridoma supernates, Developmental Studies Hybridoma Bank) supplemented with rabbit polyclonal anti-laminin antibody (2 μg/ml, Sigma) overnight at 4°C ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...
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bioRxiv - Developmental Biology 2019Quote: ... the supernatant was transferred into a new tube (and the DNA concentration determined by NanoDrop 2000 Spectrophotometer .25 μg of chromatin were used to immunoprecipitate with 3 μg of the rabbit anti H3K27me3 antibody (Millipore) previously bound to dynabeads overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH ...
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bioRxiv - Cell Biology 2019Quote: ... Bound primary antibodies were detected using peroxidase-coupled secondary anti-rabbit antibody (7074, Cell signalling) and enhanced chemiluminescence (WBLUF0500, Millipore). Blots were exposed digitally using the ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore, cat ...
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bioRxiv - Developmental Biology 2019Quote: Protein lysates of tissue isolated from the tail end of mouse embryos at E10.5 were investigated with Rabbit anti-SNX14 (Sigma, HPA017639) as previously reported (9).
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated for 60 minutes at room temperature with the rabbit antibody PRSS2 (Center) (Sigma-Aldrich, SAB 1307060) diluted 1:50 in antibody diluent with background reducing components (DAKO S 3022) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TN) and the secondary antibody HRP-conjugated anti-goat IgG from rabbit was purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Immunology 2021Quote: ... All primary antibodies were purchased from Cell Signalling Technology (Singapore) except for rabbit anti-MLC phospho-Ser19 (Sigma Aldrich, Singapore), rabbit monoclonal anti-detyrosinated tubulin and phospho-tubulin Tyr272 (Abcam ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed and incubated with HRP-conjugated goat anti-rabbit secondary antibody and developed using enhanced chemiluminescence substrate (Millipore, ON, Canada). Membrane was stripped using 0.1 N NaOH and reprobed with rabbit anti-beta actin antibody (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488 ...
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Microbiology 2022Quote: ... for 120 minutes followed by overnight incubation at 4°C with a 1/200 dilution of the poly-clonal rabbit sera and antibodies for histone H3 (final concentration 2.5 μg/mL, Sigma SAB4200651) in blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... Then the membrane was incubated with another primary serum and revealed using an anti-rabbit immunoglobulin G coupled to alkaline phosphatase (Sigma). Data were acquired from three or four independent experiments and representative immunoblots are shown ...
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bioRxiv - Immunology 2024Quote: ... before microtubule-associated protein light chain 3 (LC3)-I and LC3-II levels in cell lysates was analysed by immunoblotting (rabbit polyclonal LC3 antibody, Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... was used as the primary antibody and a goat polyclonal anti-rabbit IgG conjugated to horseradish peroxidase (Millipore, 12-348) for the secondary antibody ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... MyoD (primary Anti-MyoD Antibody, #ZRB1452, Sigma-Aldrich, and an anti-rabbit secondary antibody coupled with Rhodamine #SAB3700846, Sigma-Aldrich), DAPI (4′-6-diamidino-2-phenylindol ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate production was enzymatically quantified as NADH fluorescence (360 nm/460 nm) by the reverse reaction of L-lactate dehydrogenase (Rabbit muscle, L25005KU, Sigma) in a reaction containing 20 µL cell media ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoblots of BN-PAGE analysis were decorated with HRP-coupled goat anti-mouse or HRP-coupled goat anti-rabbit (both Sigma) as secondary antibodies (dilution ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... then transferred to 10 µL drops of equilibrated and pre-warmed rabbit culture medium covered with mineral oil (Sigma, M8410). Embryos were maintained in an incubator (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were washed with PBS three times and then incubated with secondary antibodies: horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG antibodies (Millipore, Billerica ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The primary antibodies used in immunofluorescence (IF) were rabbit anti-LvIRF antibody (Genecreate) and mouse anti-β-actin antibody (Sigma). The slides were washed with PBS six times and then incubated with diluted second antibodies of anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... We then washed the slices 5 times in PB 0.1 M and incubated them with primary antibody rabbit IgG antiGABA (Sigma A2052) diluted in PB 0.1 M 1:1000 with 1% NGS overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA reactions were subsequently performed using Duolink PLA probes for goat-mouse and goat-rabbit and Duolink In Situ Detection Reagents Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed using the Duolink® In Situ Red Kit Mouse/Rabbit (Sigma-Aldrich, Cat # DUO92101). For in vitro PLA ...