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Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using Amicon Ultra centrifugal filters with 10 kDa cut-off (Millipore) and dialyzed overnight against storage buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... Separated proteins were transferred from the gel to a polyvinylidene fluoride membrane (PVDF, Immobilon-P Millipore) using a wet blot system (Mini-PROTEAN Tetra Cell ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Biophysics 2022Quote: ... the protein was refolded via 10-fold dilution in either 5 mM oxidized glutathione (Millipore 3542) or 3.3 mM oxidized glutathione + 1.7 mM reduced glutathione dissolved in PBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 30µg protein was loaded on 10% SDS-PAGE and then transferred to PVDF membrane (Millipore) followed by blocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins were transferred onto nitrocellulose (chemiluminescence imaging) or to Immobilion FL PVDF membranes (Millipore, fluorescence imaging) using transfer buffer with 10% ethanol ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Biochemistry 2024Quote: ... The affinity captured proteins were then digested with 4 μg of MS grade trypsin (Sigma- Aldrich) in 500 μL of digestion buffer [2 M urea ...
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bioRxiv - Biophysics 2023Quote: ... The retrieved protein was concentrated to 0.5 ml with Amicon Ultra (50 kDa cutoff, EMD Millipore) and followed by size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between NINJ1 and xCT proteins was assessed using the Duolink Proximity Ligation Assay (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RIP assays were performed using the Magna RIPTM RNA-binding protein immunoprecipitation kit (Millipore, Massachusetts, USA). Cells in 15 cm dishes were lysed in complete RIP lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... expression of fusion proteins was induced for 24h by the addition of doxycycline (Sigma-Aldrich # D9891) to 5 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted immunoprecipitated protein was precipitated by adding TCA and Tween 20 (Sigma Aldrich, St. Louis, USA) to a final concentration of 10% and 0.5% respectively and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Forty micrograms of protein samples were denatured in reducing loading buffer containing β-mercaptoethanol (M3148, Sigma) and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: Immunostaining of proteins was conducted on fixed cells using 4% (wt/vol) paraformaldehyde (252549, Sigma-Aldrich) as previously described [61] ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The purified protein was concentrated to 0.6 mg/ml using an Amicon centrifugal contentrator (EMD Millipore), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were further separated on the 12% SDS-PAGE and transferred on the PVDF membrane (Millipore) using semi dry trans-blot (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Temecula, CA) and then blocked in 5% non-fat milk ...
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bioRxiv - Genomics 2024Quote: ... the system was calibrated using the Protein Standard Mix 15-600 kDa (Sigma-Aldrich, Darmstadt, Germany). Calibration was done under a flow rate of 0.5 mL min-1 with a mobile phase containing 50 mM Tris-HCl buffer (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred (300 mA, 3h) onto ethanol-activated Immobilon-P PVDF membrane (Millipore) before blocking with 3% Bovine Serum Albumin in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration for the total and cytoplasmic lysates were quantified by Bradford Reagent (B6916, Sigma Aldrich) and 10-20 μg of protein was used for western blot experiments ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Secreted JAGGED1-Fc was recovered from the culture media on Protein A agarose (Millipore, 16-125) and eluted with 100 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Immunology 2023Quote: ... The refolded protein product was concentrated by centrifugation in 3K MWCO Amicon tubes (EMD Millipore, #UFC900324) until a final concentration of at least 4 mg/mL was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates with equal amounts of proteins were transferred to anti-FlagM2 affinity gel (Sigma-Aldrich) and incubated for 1.5 hours at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were separated from beads with Corning Costar Spin-X filter units (#CLS8162, Sigma-Aldrich) and stored at -20°C until further processing.
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were resolved on SDS-polyacrylamide gels and transferred onto PVDF Immobilon-FL membranes (Millipore, IPFL00010). Membranes were blocked with Intercept blocking buffer in PBS (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were separated by SDS-PAGE (15%) and transferred to PVDF membranes (Immobilon-FL, Millipore). Membranes were incubated with Intercept Blocking Buffer (Li-COR) ...
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bioRxiv - Pathology 2023Quote: ... Proteins were separated using 7.5% SDS-PAGE gels and blotted onto a PVDF membrane (Millipore, 0.45μm) and blocked followed by incubation with anti-FAK (CST) ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore, 50 kDa MWCO) and flash frozen in liquid nitrogen.
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in the samples was quantified using the Bradford assay (Sigma). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was then applied to a Protein G Sepharose (Millipore Sigma, #GE17-0618-01) containing column (5ml polypropylene columns ...
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bioRxiv - Microbiology 2023Quote: ... protein lysates were added to 50 ul of prewashed ANTI-FLAG M2 Affinity Gel beads (Sigma) and incubated at 4°C overnight with gentle rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by standard SDS-PAGE and transferred to a PVDF (polyvinylidine difluoride) membrane (Millipore). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitated protein-DNA complexes were prepared using the Magna ChIP A kit (Millipore, Burlington, MA, USA) according to the manufacturer’s instructions ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Cells were lysed in cold lysis buffer (CytoBuster Protein Extraction Reagent, Novagen Inc., Madison, WI, USA) in the presence of a protease inhibitor cocktail (Complete Mini ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred overnight onto methanol activated polyvinylidene difluoride membrane (PVDF, Sigma-Aldrich, MO, USA) in Transfer Buffer (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: Total protein content in all cell extracts was determined using the QuantiPro BCA Assay Kit (Sigma).
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bioRxiv - Cell Biology 2023Quote: HA-METTL3-Flag protein was purified from 293T by using anti-HA magnetic beads (Sigma Aldrich) as described in this method ...