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Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... α-tubulin antibody was used as loading control (mouse anti-α-tubulin, 1:10000; Sigma-Aldrich cat#T6074, RRID: AB_477582). Antibodies were functionally validated by Cell Signalling Technology and Sigma-Aldrich companies ...
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bioRxiv - Molecular Biology 2022Quote: ... HRPA-1::AID*::TEV::FLAG was detected using Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% triton-X 100 in PBS at room temperature for one hour and incubated overnight in 5% FBS and 0.1% triton-X 100 in PBS at 4°C with the following antibodies: anti-LIMCH1 (1:200, catalog number HPA063840, Sigma), anti-mLIMCH1 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Molecular Biology 2024Quote: The following monoclonal and polyclonal antibodies were used: anti-β-actin (WB: 1 µg/ml, AC-15/A1978, Sigma-Aldrich), anti-Miro1/2 (WB ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were incubated with mouse anti-FLAG primary antibody (1:1000 dilution in PBS + 0.1% BSA; Sigma– Aldrich, F3165) at room temperature for 30 min and washed three times with PBS + 0.1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts of puromycin pulsed and non-puromycin pulsed control cells were subject to immunoblot (as above) using anti-puromycin antibody (clone 12D10, 1:1000, Sigma).
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bioRxiv - Cell Biology 2024Quote: ... cultures were incubated for 1 h at room temperature with primary antibodies as follows: mouse anti-laminA/C (Sigma SAB4200236), mouse anti-phospho-histone H2AX (Merck 05-636) ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were then transferred to a ChAT primary and TrkA primary antibody solution with blocking buffer (1:200 dilution goat anti-ChAT polyclonal AB143, Millipore EMD ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Immunology 2024Quote: Mast cell cultures were stimulated with the following for the indicated time periods: 1 µg/ml IgE-anti DNP antibody (Sigma) and 0.2 µg/ml HSA-DNP (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA for 15 min at room temperature before incubating with primary anti-Flag antibody (diluted with PBS containing 5% BSA at a ratio of 1:300, Sigma) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Cancer Biology 2023Quote: ... γ-H2AX foci were detected with mouse monoclonal antibody anti-phospho-Histone H2AX (Ser139) (1:1000 dilution; Cat #05-636, Millipore) and goat anti-mouse IgG secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Then the slides were incubated in the above blocking buffer overnight at room temperature with gentle rocking with the primary antibodies as indicated guinea pig anti-ASIC3 (1:2000, Millipore), rabbit anti-TRPV1 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... Then cells were resuspended in the BD-FACS staining buffer and labelled through anti-human TRA-1-60 antibody (Millipore) and anti-mouse IgM control ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were rehydrated in phosphate buffered saline (PBS) and incubated overnight with anti-NaV1.7 antibody (1/200; Cat #: MABN41, Sigma-Aldrich) in PBS with 3% bovine serum albumin at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... S4 a similar protocol was used but the primary antibody mix consisted of mouse anti-GFP 1:1000 (Sigma G6539), rabbit anti-RFP 1:5000 (Rockland 600-401-379) ...
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bioRxiv - Molecular Biology 2023Quote: ... The process was the same as in fibroblasts and the primary antibodies were anti-human NANOG (1:100, Millipore #AB5731) and SSEA4 (1:25 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Dig epitopes were detected with alkaline phosphatase-coupled anti-Dig fab fragments antibody at 1:2500 (Millipore Sigma, cat. #11093274910), and the signal revealed with Nitro Blue Tetrazolium chloride/5-Bromo-4-Chloro-3-Indolyl Phosphate ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 48 h in the primary antibodies: Monoclonal mouse anti-parvalbumin (1:5000; P3088; Sigma-Aldrich) and Wisteria floribunda lectin (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated macular retinal nuclei were stained with a mouse anti-NeuN monoclonal antibody (1:5000, Alexa Flour 488 Conjugate MAB377X, Millipore, Billerica ...
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bioRxiv - Molecular Biology 2023Quote: ... CRHR2α expression levels in the stably transfected cell lines was assessed by Western blot using a mouse monoclonal anti FLAG -antibody (1:5000 dilution; SIGMA). All the experiments were performed at least 3 times with 3 individual clones (clones 4 ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was blocked for 1 h in 5% milk in PBS-T then incubated overnight with an anti-flag antibody (Sigma) or actin antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Genomics 2023Quote: ... then stained with anti-NeuN antibody (directly conjugated to Alexa Fluor 488; Millipore cat. No. MAB377X, clone A60; 1:1,250) and an anti-rabbit IgG Alexa Fluor 647 antibody as a negative control for 30min ...