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Citations for Millipore Sigma :
8601 - 8650 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Genomics 2019Quote: ... we blocked coverslips with 5% BSA in PBT for 1 hour at room temperature and then incubated coverslips with properly diluted primary antibody (mouse M2 monoclonal ANTI FLAG, 1:800, F1804, Sigma; rabbit polyclonal Tom20 ...
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bioRxiv - Physiology 2019Quote: ... IHC was performed by incubating sections (n=5 per each group) with the primary antibody (anti-mUCP-1, U6382, Sigma), followed by a secondary antibody conjugated with HRP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... The sheared chromatin was immunoprecipitated overnight using the following antibodies and dilutions: anti-LexA BD (Millipore 06-719, 1:300) Anti-H3K27me3 (Millipore ...
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.05% (v/v) Tween-20 in PBS) and incubated with primary antibody anti-LIG3 (1:150, Sigma-Aldrich, #HPA006723) in staining buffer for 2hr at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... were isolated from 2-week-old dPPRrbcL-1 or Col-0 plants and incubated with monoclonal anti-HA antibodies (Sigma). IgGs were captured with Protein A DynaBeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were again washed thrice followed by incubation with 1:5,000 dilution of horse radish peroxidase (HRP) conjugated anti-guinea pig IgG secondary antibody (Sigma-Aldrich) or 1:2,000 dilution of HRP conjugated anti-mouse IgG secondary antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Protein level of GTP-bound ARF6 and total ARF6 in cell lysates was detected by Western blot with an anti-ARF6 antibody (1:1000 in 5% milk) (Sigma).
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were then labeled in a solution of anti-NeuN antibody conjugated to Alexa Fluor 488 (A60, Millipore, 1/1000) and 0.1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Physiology 2020Quote: ... Immunofluorescence staining was performed using sheep anti-α-MSH antibody (diluted 1:1,000 in 0.1 M PB, ab5087, Millipore Sigma), and rabbit anti-AgRP antibody (diluted 1:1,000 in 0.1 M PB ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... The permeabilized cells were washed with PBS and sequentially incubated anti-DENV primary antibody (dilution 1:400 of Mab8705, Millipore) and anti-Mouse Alexa-488 conjugated secondary antibody (dilution 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed and incubated with primary antibody diluted in 5% BSA/TBST (1:10,000 anti-Flag (Sigma-Aldrich) or anti-Beta lactamase (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... probed with a 1:10,000 dilution of an anti-guinea pig-horseradish peroxidase-conjugated secondary antibody (Sigma; product no. A5545) for 30 min at ambient temperature ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes and then incubated for 48 hours at room temperature in goat anti-ChAT primary antibody (1:1000, AB144P, Millipore). Following rinse ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...