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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti-Centrin-1 (1:500 Sigma 04-1624); Mouse anti-TACC3 (1:1000 Abcam ab56595) ...
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bioRxiv - Biophysics 2021Quote: ... the cells were collected and blocked with 5% BSA in PBS at RT for 15 min and incubated with primary anti-Flag antibody (1:300, Sigma-Aldrich) at RT for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... filtered through a cell strainer and stained with a 1:1000 dilution of the anti-NeuN antibody (clone A60, Alexa Fluor 488 conjugated, Millipore MAB377X) for 45 minutes by rotation in the dark at 4°C after adding BSA to a final concentration of 0.1% ...
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: ... The proteins in both soluble fraction and membrane fraction were separated in 12% SDS-PAGE gel and analysed by western blotting using anti-GFP polyclonal antibody (1:4000; Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... was then added to cells for 20 minutes and subsequently replaced with an anti-FLAG antibody (1:1000 in blocking solution) (Cat #F3165, Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated overnight at 4°C on a rocking platform with the primary antibody anti-H3K9ac (1:1,000; Sigma, cat. # H9286) or anti-H3K9bhb (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: Innervation analysis was done on regenerating limb and flank sections to quantify innervation abundance using the Mouse Monoclonal Anti-Acetylated Tubulin antibody (1:200 dilution – Sigma Aldrich), followed by the Goat-Anti-Mouse IgG Alexa Fluor 488 (1:200 dilution – Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... GPR126 and empty vector-transfected cells were stimulated with the indicated concentrations of anti-HA antibody (H3663, stock concentration 1 mg/ml, Sigma-Aldrich), anti-HA Fab fragment (stock concentration 1 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated again in blocking solution with primary antibody against choline acetyltransferase (ChAT; Millipore, AB144P, goat anti-ChAT, 1:1000) for 5 nights at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Biophysics 2020Quote: ... a solution of 20 µg ml-1 biotinylated anti Mouse IgG antibody (#115-065-003-JIR-2ml STRATECH SCIENTIFIC LIMITED) and 20 µg ml-1 anti-Dynein Intermediate chain antibody (#MAB16818 Sigma-Aldrich). Unreacted streptavidin binding sites were then blocked using biotin-BSA (#PN29130 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4°C with primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500; Millipore Cat# AB144P, RRID:AB_2079751), mouse anti-parvalbumin (PV ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated in TN buffer (150 mM NaCl and 100 mM Tris pH= 7.5 in water) and incubated for 30 min in 1:2000 HRP-coupled anti-DIG antibody (Sigma-Aldrich, 11207733910). Following the incubation ...
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bioRxiv - Microbiology 2021Quote: ... DivK protein separated based on its phosphorylation state was transferred to a PVDF membrane and detected using a chemiluminescent substrate (SuperSignal West PICO PLUS, Thermo Pierce) after incubation with mouse anti-Flag (1:10000) primary antibody (Sigma, F1804) and a polyclonal goat anti-mouse HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Bioengineering 2020Quote: ... the primary antibody that was used was mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend (EMD Millipore, MAB8251, 1:250). Images were taken at 10x magnification using a Zeiss Axio Observer and Axiocam 503 mono camera (Zeiss).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with a goat anti-choline acetyltransferase antibody (ChAT; 1:1000; Millipore Cat# AB144P, RRID:AB_2079751) in Tris-Triton buffer containing 2% donkey serum and 0.2% Triton X100 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Remaining cell lysate was incubated with pre-washed (three times in 1 ml lysis buffer) 20 μl of antibody-conjugated beads/resin (FLAG: EZview Red ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich; HA ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... in Triton PBS containing 1% v/v NDS and a mixture of between two and four of the following primary antibodies: goat anti-choline acetyltransferase (ChAT; 1:500 dilution; AB144P, Millipore, RRID:AB_2079751); goat anti-forkhead box protein P2 (FoxP2 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000; EMD Millipore, cat. no. 05-1308, clone apu3), anti-actin (1:6,000 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was removed and replaced with 500 μl of a solution containing a 1:500 dilution of anti-tyrosine hydroxylase antibody (Millipore Sigma). Slices were incubated overnight with side to side rotation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with the primary antibodies (12h at 4 °C): anti-ChAT (1:500, goat, Sigma-Aldrich, AB144P). Then the sections were washed in PBS five times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Molecular Biology 2022Quote: ... the rest of supernatant (250 μL, IP) was transferred into another tube and incubated with anti-Flag antibody (1:100, Sigma-Aldrich) or control immunoglobulin G (IgG ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated ON at 4°C with the following primary antibodies diluted in blocking buffer: anti-FLAG (1:200, M2 clone, catalog no. F1804, Sigma-Aldrich); anti-FLAG (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Biophysics 2022Quote: ... The tyrosinated tubulin level was detected by western blot using monoclonal anti-tyrosinated tubulin antibody (clone YL1/2; 1:2500 dilution; EMD Millipore) as primary antibody and a goat anti-rat secondary antibody (alkaline phosphatase-conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... Neural dendrites were detected with the neurofilament marker anti-SMI 311 mouse monoclonal antibody (1:600, Millipore Bioscience Research Reagents, USA). The day after ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2020Quote: ... in TBS with 0.4 % Triton X-100 and incubated with a mouse anti-GFAP antibody (1:500, clone G-A-G, Sigma: G 3893) in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at RT with a combination of two primary antibodies: a mouse monoclonal anti-PCNA (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% non-fat milk and incubated with the following antibodies: anti-FLAG (1:1000, Cat. #F1804; Sigma-Aldrich), and anti-HA (1:1000 ...