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Citations for Millipore Sigma :
8601 - 8650 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with 100 µL of Triton X-100 in water (Sigma Aldrich X100). Lysates were titrated in PBS + 0.05% Tween-80 and plated on 7H10 ...
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bioRxiv - Biophysics 2022Quote: ... and Amicon® Ultra centrifugal filters 100 kDa (100 K, Merck Millipore Ltd., Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Seven replicate 100 mM stocks were made in 100% DMSO (Sigma Aldrich, St. Louis, MO) and were stored in 0.5 ml aliquots in 1.10ml polypropylene ...
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bioRxiv - Biochemistry 2021Quote: ... Proteasome inhibitors were used as 100 nM bortezomib (Selleck Chem) and 100 nM epoxomicin (Millipore). Ferroptosis was inhibited using 10 μM ferrostatin-1 (Selleck Chem) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% NP-40) supplemented with 100 mM PMSF and 100 mM DTT (Dithiothreitol, Sigma Aldrich). Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies ...
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bioRxiv - Genetics 2023Quote: ... 5% triton X-100 solution was prepared by diluting Triton X-100 purchased from Sigma in distilled water ...
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bioRxiv - Cell Biology 2020Quote: ... were subsequently washed three times in kinase buffer (25 mM Tris-HCl pH 7.4, 100 mM NaCl, 10 mM MgCl2, 50 μg/ml bovine serum albumin [Sigma], 0/1mM EGTA, 0.1% β-mercaptoethanol). Beads (10 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were transduced with lentivirus encoding the ABE reporter at varying dilutions (1:10, 1:100, 1:500, and 1:1000) in the presence of 8 μg/ml polybrene (Millipore Sigma Cat. #: TR-1003-G). Three days after transduction ...
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bioRxiv - Neuroscience 2021Quote: Regular drinking water was removed 24hr prior to NMDA injections and replaced with water containing 5-ethynyl-2′-deoxyuridine (EdU; Sigma; 50 mg/100 mL dH2O) and EdU water was replaced every third day ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 5 mL autoclaved LB broth (cat. no. J8331L, Quality Biological Inc., Gaithersburg, MD, USA) supplemented with 100 μg/mL ampicillin (cat. no. A5354, Sigma-Aldritch, St. Louis, MO, USA). Liquid cultures were incubated overnight at 37 °C with shaking ...
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bioRxiv - Neuroscience 2022Quote: ... 4 well chamber slides (177437, Thermo Fischer Scientific) were coated by incubating for 12 h with filter sterilized poly-L-lysine (P8920, Sigma-Aldrich; 100 μ g/ml) in borate buffer (boic acid 3.1g/l ...
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bioRxiv - Microbiology 2022Quote: ... the peptide and the exudates were incubated for 10 min at 100 °C or treated with 1 mg/mL proteinase K (Sigma Aldrich, Saint Louis, MO, USA). Plates were incubated at 25 °C in the dark for 14 h ...
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bioRxiv - Microbiology 2022Quote: ... 2×10⁴ spores/mL (in 100 µl) were added to a CytoOne® 96-well plate (StarLab, Brussels, Belgium) containing 1xRPMI-1640 medium (Sigma Aldrich, St. Louis, MO), 165 mM MOPS buffer (pH 7.0) ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Cell Biology 2022Quote: ... The old media in wells was replaced with transduction media consisting of 2 mL complete M106 mixed with an appropriate amount of respective lentivirus cocktail and 2 μL of 100 mg/mL Polybrene (Millipore Sigma Cat. TR-1003-G). The cells were left to transduce for 12 hours/overnight ...
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bioRxiv - Cell Biology 2022Quote: ... was prepared in USP30 activity assay buffer (50 mM Tris base pH 7.5, 100 mM NaCl, 0.1 mg/ml BSA [Sigma, A7030], 0.05% Tween 20, 1 mM DTT) and 15 μL dispensed into compound-containing assay plate using the Dragonfly Discovery liquid dispenser and incubated for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/mL horseradish peroxidase [Millipore-Sigma] with or without immune elicitors). Light emission was measured using a TECAN Spark® plate reader.
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bioRxiv - Neuroscience 2024Quote: ... The supernatants of the negative control samples were saved as Input while reverse crosslinking was performed on the H4K8ac immunoprecipitated chromatin using elution buffer (100 mM NaHCO3 & 1 % SDS) supplemented with proteinase K (Millipore Sigma, 3115887001, 20 mg/mL) and incubated at 68 °C in a thermomixer at 1300 rpm for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells were removed after 2 days and replaced with complete medium with ascorbic acid (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA). The media was replaced with fresh complete media with ascorbic acid every other day ...
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bioRxiv - Neuroscience 2023Quote: Human neuroblastoma cells SH-SY5Y cells (ATCC® CRL-2266™) were cultured in T75 flasks for propagation and then plated onto 100 μg/ml polyD-lysine (Millipore, A-003-E) coated 96-well glass bottom plates for imaging ...
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bioRxiv - Systems Biology 2023Quote: ... We applied a pressure of 50 kPa to fully perfuse the cell chambers with 100 ng/mL fibronectin solution (Sigma-Aldrich Chemie N.V., Zwijndrecht, Netherlands). In order to de-bubble the device ...
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bioRxiv - Developmental Biology 2023Quote: ... COCs were placed in a 100-μl droplet of human tubal fluid (HTF) medium supplemented with 4 mg/ml BSA (Sigma-Aldrich, St. Louis, MO, USA)28 ...
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bioRxiv - Microbiology 2023Quote: ... cenocepacia in a 3:1 ratio on SOB plates containing 100 μg/mL of 2,6-diaminopimelic acid (DAP, Sigma-Aldrich, St. Louis, MO, United States) for 2 days at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% ITS (5µg/ mL insulin, 5µg/mL transferrin, 5ng/mL selenium; Sigma), 100µg/mL sodium pyruvate and 1% penicillin/streptomycin sulfate (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and 2 µM Antimicyn A / 1 µM Rotenone (all Sigma-Aldrich; Missouri, USA). The data were analyzed using Wave 2.6 Software ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The fish were anesthesized using 32.5 μl from a 10% stock solution of ethyl-p-aminobenzoate (Benzocaine E1501, Sigma-Aldrich) dissolved in 100% ethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 µg of protein lysates were loaded onto an SDS-PAGE gel and wet-transferred to 0.45 µM Immobilon-P PVDF membrane (Millipore, IPVH00010) at 200 mA for 90 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Molecular Biology 2021Quote: Pellets of cells expressing SFB- or BioID2-tagged viral genes were subjected to lysis with NETN buffer (100mM NaCl, 1mM EDTA, 20mM Tris-HCl, and 0.5% Nonidet P-40) with protease inhibitor cocktail (Sigma-Aldrich) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... The media used for the above cell lines were supplemented with 10% fetal bovine serum (FBS, Seradigm) and 1% penicillin-streptomycin (P/S, Sigma). The lung adenocarcinoma cell line ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred onto polyvinylidene fluoride membrane (PVDF, Hybond-P, Amersham) in transfer buffer containing 12 mM Tris (Sigma), 96 mM glycine (BDH ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... for the final differentiation in neural differentiating medium (NDM) (Neurobasal 1% NEAA, 1% N2 supplement and 1% P/S) supplemented with 1µM of cAMP (Sigma-Aldrich) and 10 ng/mL of BDNF ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore) according to standard procedures (Taylor ...
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bioRxiv - Neuroscience 2019Quote: ... 300 μl per well and were lysed with 300 μl per well of 1% Nonidet P-40 lysis buffer containing proteinase inhibitor (1:10,000; Sigma-Aldrich). Samples were further homogenized using a Q125 sonicator (Qsonica ...
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bioRxiv - Bioengineering 2019Quote: ... re-suspended in freezing medium (RPMI-1640 supplemented with 20 % FBS, 1 % P/S, and 10 % Dimethyl sulfoxide (Merck Millipore)) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by loading whole cell lysates onto an 8%-10% SDS-PAGE gel for electrophoresis and then transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were incubated with primary antibodies in TBST (TBS ...
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bioRxiv - Immunology 2021Quote: ... A total of 105 cells or 20 mg protein was resolved by SDS-PAGE and transferred to Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk in TBS-T (25 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Biochemistry 2020Quote: SDS samples were run on 10 % SDS-PAGE and transferred to 0.45 μm Immobilon-P PVDF Membrane (Millipore Sigma, IPVH00010) and immunoblotted using anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Tunica muscularis strips were fixed with 4 % (w/v) phosphate buffered p-formaldehyde (PFA; Sigma-Aldrich, St. Louis, MO, USA) for 20 min and rinsed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-γH2AX (JBW301, 1:1000), anti-P-Ser15-p53 (1:1000) and anti-ATM Ser1981 (10H11.E12, 1:200) were from Millipore. Anti-P-KAP1Ser824 (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... mitochondrial or synaptosomal fractions were prepared in Laemmli buffer and separated by SDS-PAGE before transfer to a PVDF membrane (Immobilon-P, Millipore). Membranes were blocked in 5 % non-fat milk in TBS-Tween 20 (0.1 % ...
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bioRxiv - Neuroscience 2019Quote: ... 20 μg per lane Figure S3a) were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Cell Biology 2021Quote: ... cell-bound AP activity was eventually revealed by incubation with the chromogenic soluble substrate p-nitrophenylphosphate (Sigma-Aldrich, Catalog # P7998) and measured by a multi-well spectrophotometer (absorbance at 405 nm).
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bioRxiv - Plant Biology 2021Quote: ... Absolute quantification of phenolic esters was performed on HPLC-UV with external calibration curves of corresponding free hydroxycinnamic acid (i.e. p-coumarate, caffeate and ferulate; Sigma-Aldrich). Kinetic parameters were calculated with nonlinear Michealis-Menten regression using the GraphPad Prism v4.8 software (Fig ...
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bioRxiv - Microbiology 2020Quote: ... Eight micrograms of protein per lane were separated by 12% SDS polyacrylamide gel electrophoresis and blotted onto Immobilon-P nylon membranes (Millipore). After transfer ...
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bioRxiv - Plant Biology 2020Quote: Total protein extract from the different plants was separated on 10% SDS-PAGE gel and transferred to a PVDF membrane (Immobilon-P Transfer membrane, Millipore) by semi-dry blotting in 25 mM Tris–HCl ...
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bioRxiv - Systems Biology 2021Quote: Cell fractions were thawed on ice and endogenous kinases were inhibited with 5′-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA; Sigma-Aldrich) in DMSO at a final concentration of 1 mM FSBA and 10% DMSO in Tris-HCL ...