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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were resolved under denaturing and reducing conditions using 4 to 12% bis-tris gels (NuPAGE) and transferred to a polyvinylidene fluoride membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk and incubated with CAMKIIα (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: Frozen leaf tissue was ground in two volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich], 1% plant protease inhibitor cocktail [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2023Quote: ... overnight P. aeruginosa PA14 strains (Supp. Table 2) were grown under aerobic conditions in Luria-Bertani broth (LB, Millipore Sigma) at 37°C for 16-20 hours at 300 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electrophoretic separated on 4-20% SDS-PAGE precast gels (Beyotime) and transferred to a Hybond P polyvinylidene difluoride membrane (Millipore). The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Organoids were washed in PBS and then incubated at 37°C with 200μl /well in Gly-Pro p-nitroanilide hydrochloride (Sigma G0513) dissolved in PBS at a concentration of 1.5mM (or PBS alone in control wells) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies against p-LRRK2 and total LRRK2 were diluted in EMD signal boost solution 1 (Millipore Sigma, Burlington, MA). After washing ...
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bioRxiv - Molecular Biology 2023Quote: ... were subjected to SDS-PAGE with 8% or 15% Tris-Glycine gels and transferred to Immobilon- P PVDF membranes (Millipore) using a TransBlot SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS/PAGE on a 12% (vol/vol) acrylamide gel and transferred to PVDF Immobilon-P membranes (Millipore). Primary antibodies specific for caspase-4 (4450S ...
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bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of product was calculated by linear regression equation given on the standard curve performed by the reference compound p-nitrophenol (Sigma). One unit of enzyme activity was defined as 1 μmol of p-nitrophenol produced per minute under the assay conditions.
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bioRxiv - Biochemistry 2023Quote: ... 1% Nonidet P-40) in the presence of a protease inhibitor cocktail and phenylmethanesulfonyl fluoride (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Cancer Biology 2024Quote: ... samples of the whole cell extract and immunoprecipitates were separated by SDS-PAGE and transferred onto 0.45 μm Immobilon-P PVDF membranes (Millipore Sigma). Membranes were then blocked in 5% nonfat dried milk / PBST for 30 minutes at room temperature and incubated with the indicated primary antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Biochemistry 2024Quote: ... for 3 hours at 15° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Biochemistry 2024Quote: ... for 30 minutes at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Molecular Biology 2024Quote: ... 786-O, 769-P, A704, and RCC- FG2 (Cell Lines Service, CLS GmbH) cells were cultured in RPMI 1640 medium (Sigma) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Biochemistry 2024Quote: Cell lysates were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions and transferred to polyvinylidene difluoride membranes (Immobilon P, IPVH00010, Millipore). Primary antibodies used were anti-HA High Affinity ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Plant Biology 2024Quote: ... for 5 minutes and the supernatant was separated on a 12% SDS-PAGE gel and subsequently transferred onto a PVDF membrane (Immobilon-P, Millipore). Immunodetection of the proteins was performed according to standard protocols using the antibodies detailed (Table 3) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted in 10 µL of mounting medium (80% glycerol, 0.1 M Tris pH 8.8, 0.05% p-phenylenediamine dihydrochloride (Sigma, cat. no. P1519)) and stored at −20°C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immobilon-P, Millipore, Bedford, MA). Membranes were blocked with PBS plus 0.1% Tween 20 (PBST ...
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bioRxiv - Genetics 2024Quote: ... and incubated for 1 hour at 30°C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Neuroscience 2024Quote: ProBDNF digestion samples were run on 15% SDS-PAGE gels and electro-transferred onto polyvinylidene difluoride membrane (Immobilon-P, Millipore), using semi-dry method ...
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bioRxiv - Physiology 2024Quote: ... the pancreas was perfused via the pancreatic duct using an ice-cold collagenase solution (Collagenase P, 11213865001, Merck/Sigma Aldrich) with a concentration of 0.63 mg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5–40 μg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). GFP was detected using the anti-GFP antibody (Mouse monoclonal ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from wt and mutant embryos (n=30, for each genotype) using TRIzol (Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed with gentle agitation for 45min at room temperature with fresh 2mM Di(N-succinimidyl) glutarate (Sigma) in PBS ...
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bioRxiv - Genomics 2022Quote: ... or 6.15 mM [0.1% w/v] N-glycolylneuraminic acid (Neu5Gc) (all purchased from Sigma-Aldrich, Taufkirchen, Germany) was added ...
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bioRxiv - Physiology 2022Quote: ... Liver glycogen content was measured using amyloglucosidase enzyme (catalog n°A7095, Sigma-Aldrich, Saint-Louis, MO, USA) assay according to Roehrig and Allred method (Roehrig et Allred 1974) ...
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bioRxiv - Neuroscience 2021Quote: ... ablation efficiency was confirmed using the fluorescent dye 2-4-(dimethylamino)styryl-N-ethylpyridinium-iodide (DASPEI)(Sigma). Larvae were immersed in 0.005% DASPEI in E3 for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... a 500 μl 50% suspension of 3 times pre-washed streptavidin agarose beads (Novagen, Cat. N°69203) (i.e. ...
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bioRxiv - Bioengineering 2019Quote: ... sulfo-N-hydroxysuccinimide (sulfo-NHS) and Ni-NTA were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Neuroscience 2019Quote: ... Mice were genotyped by PCR on tail DNA using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or treated for 3 min with 30 µM N-Methyl-D-aspartic acid (NMDA) (Sigma-Aldrich, M3262). After 30 min slices were incubated for 10 min with AcTEV or vehicle control ...
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bioRxiv - Cell Biology 2021Quote: ... We measured the lifetime of free NADH in solution (Sigma Aldrich n. N8129, St. Louis, MO, USA) to calculate the fraction of bound NADH (Figure S6B) ...
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bioRxiv - Bioengineering 2021Quote: ... scaffolds (n = 3) were submerged into 500 μL of 4 M guanidine hydrochloride (GuHCl, Sigma-Aldrich, Canada) buffer supplemented with a protease inhibitor (Roche Applied Science ...
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bioRxiv - Bioengineering 2021Quote: ... 48 h and day 21 hBMMSC-seeded scaffolds (n=3) were fixed in 4% paraformaldehyde (Sigma, Canada) for 1 hour and submerged in gradient sucrose solutions from 10% to 30% ...
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bioRxiv - Microbiology 2021Quote: ... The minimal medium was supplemented with 5 mM 2-(N-morpholino) ethanesulfonic acid (Mes: Sigma Chemical Co.) adjusted to pH 6.4 with NaOH as buffer and 0.5% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... animals (n=30) were injected (i.p.) with thioacetamid (TAA) (200 mg/kg in normal saline, Sigma-aldrich) twice per week for 8 weeks ...
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bioRxiv - Biophysics 2020Quote: ... the Alexa-Fluor dyes conjugated to N-hydroxysuccinimide (NHS) were dissolved in dimethylsulfoxide (DMSO, Sigma-Aldrich, 276855) to a final concentration of 10 mg ml-1 ...
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bioRxiv - Pathology 2022Quote: ... a group of mice (n = 19) received daily tyrphostin AG1478 (10 mg/kg mouse, Millipore Sigma, T4182) via oral gavage ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... ADC scaffolds (n = 3) were gently agitated in 4 M guanidine hydrochloride buffer (GuHCl, Sigma-Aldrich, Canada) supplemented with a protease inhibitor (Roche Applied Science ...
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bioRxiv - Biophysics 2022Quote: ... CAFs (Vitro Biopharma Cat. n. CAF08) were cultured in DMEM medium (D5671, Sigma-Merck KGaA, Darmstadt, Germany) supplemented with 10% FBS (F7524 ...