Labshake search
Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... RD4 (Sigma-Millipore 1:100) and anti-4R (Cosmo Bio 1:400).
-
bioRxiv - Cancer Biology 2024Quote: ... α-OLIG2 (1:100, ab9610, Sigma), α-Laminin (1:100 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1:100 Catalase (Sigma, C3155), 0.8% D-Glucose ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 µM dbcAMP (Sigma-Aldrich)] ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1% Triton X-100 (Sigma) in PBS 1X (Gibco)) ...
-
bioRxiv - Plant Biology 2024Quote: ... 100 µl carboxymethylcellulose (CMC, Sigma) 2 % (w/v ...
-
bioRxiv - Developmental Biology 2024Quote: ... NG2 (1:100, Millipore, AB5320), Ki67-488 (1:250 ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 μm picrotoxin (Sigma-Aldrich), and 20 μm strychnine (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... Triton-X-100 (Sigma T9284) was added (final concentration 1% ...
-
bioRxiv - Cell Biology 2019Quote: ... Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 µm Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare) ...
-
bioRxiv - Cell Biology 2019Quote: Chromatin preparations were separated on a 18% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) Immobilon-P membranes (Millipore). Immunoblots were performed with enhanced chemiluminescence (ECL ...
-
bioRxiv - Microbiology 2020Quote: ... in M9 minimal medium supplemented with 0.4% D-glucose and 10 mM para-chloro-phenylalanine (p-Cl-Phe; Sigma) for B ...
-
bioRxiv - Immunology 2021Quote: ... Protein lysates (25 μg) were resolved by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Sigma-Aldrich, IPVH00010). Membranes were blocked in 5% nonfat milk in TBST buffer at room temperature for 1 hour ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
-
bioRxiv - Biochemistry 2019Quote: Protein samples of in vitro translation reactions and subsequent purifications were separated on SDS-PAGE at neutral pH condition (pH 6.8, for purified protein samples) and were transferred on PVDF membrane (Immobilon-P, Millipore). After blocking with 5% skim milk in PBS-T ...
-
bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
-
bioRxiv - Microbiology 2021Quote: ... 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore). The membranes were blocked with 5% bovine serum albumin in 1XPBS with 0.5% Tween 20 (PBST ...
-
bioRxiv - Microbiology 2021Quote: ... and applying lysis buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 1% Nonidet P-40 (all from Sigma)) supplemented with EDTA-free protease inhibitor mixture cocktail (Roche) ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were rinsed twice in DPBS then simultaneously blocked and permeabilized with B/P buffer [DPBS with 0.1% Tween 20 (P2287; Sigma), 0.1M glycine (161-0718 ...
-
bioRxiv - Neuroscience 2020Quote: ... 20 μg total protein/samples were loaded in each lane of each gel on 7.5-10% SDS-polyacrylamide gel and then transferred to nitrocellulose membrane (Immobilon-P, Millipore). After blocking ...
-
bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
-
bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
-
bioRxiv - Physiology 2021Quote: ... The procedure consisted of using one mM of BAPNA (Nα-benzoyl-DL-arginine-p-nitroanilide hydrochloride, Sigma B-4875) substrate dissolved 500 µL of DMSO in 50 mM Tris-HCl buffer ...
-
bioRxiv - Biochemistry 2019Quote: The proteins in an SDS-polyacrylamide gel were transferred onto an Immobilon®-P PVDF transfer membrane (Millipore Sigma) by electroblotting at 250mA for two hours in ice cold transfer buffer (25mM Tris ...
-
bioRxiv - Genetics 2019Quote: ... Samples were run on a 9% (w/v) SDS-polyacrylamide gel and transferred to an Immobilon-P membrane (Millipore). Membranes were incubated for 1 hour at room temperature in 1xTBST + 5% non-fat milk with primary antibodies (1:2000 α-Myc 9E10 ...
-
bioRxiv - Biochemistry 2020Quote: ... A 96-well dot blot apparatus (Schleicher & Schuell) was set up with a 0.45-μm-pore-size polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore), and each dot was rinsed with 500 μl of TBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Both the cell and CM plates were treated with para-nitrophenylphosphate (p-NPP, 100mM) (Sigma-Aldrich, St. Louis, MO) substrate for one hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The plates were washed with PBST and reactions were developed with p-nitrophenyl phosphate substrate (PNPP; Sigma-Aldrich, N2765). Plates were read at 405 nm absorbance ...
-
bioRxiv - Plant Biology 2020Quote: ... Leaf tissue was ground in extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.2 % Nonidet P-40 [Sigma-Aldrich] ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Samples were derivatized in RT for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma Aldrich) in ACN (Hu et al. ...
-
bioRxiv - Physiology 2020Quote: ... osteoblast differentiation capacity was assessed by alkaline phosphatase (ALP) staining using SIGMAFAST™ p-Nitrophenyl phosphate tablets (N1891, Sigma). Colonies with more than 10% of ALPpositive cells were considered as colony-forming units–osteoblasts (CFU-OBs) ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
-
bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma] ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein samples were separated on a 12.5% SDS-PAGE gel and transferred to a polyvinylidenedifluoride (PVDF) membrane (Immobilon-P, Millipore). Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Microbiology 2020Quote: ... Detergent-extracted cytoskeletons were prepared by incubating the biotin treated cells in PEME buffer with protease inhibitors plus 0.5% Nonidet P-40 (Sigma) for 30 min at room temperature followed by centrifugation at 3400 × g for 5 min at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... The plates were washed with PBST and reactions were developed with p-nitrophenyl phosphate substrate (PNPP, Sigma-Aldrich, N2765). Plates were read at 405 nm absorbance and antibody titers were calculated as the highest serum dilution with an OD value above 2 times the average OD of the negative controls.
-
bioRxiv - Microbiology 2021Quote: ... The cells yielded in this way were cultured in DMEM supplemented with 10% FBS and 1% P/S (Sigma). Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T ...
-
bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
-
bioRxiv - Molecular Biology 2021Quote: ... were lysed with a Potter-Elvehjem homogenizer in lysis buffer (10 mM Tris pH 6.8, 10 mM MgCl2, 0.2% Nonidet P-40, 1 mM PMSF (Sigma-Aldrich), 4 mM benzamidine (Sigma-Aldrich) ...
-
bioRxiv - Plant Biology 2022Quote: ... 40 μg total proteins were loaded into 10% acrylamide gels and transferred to Immobilon-P membrane (Millipore, Cat. IPVH00010). The antibody were diluted as follows ...
-
bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
-
bioRxiv - Molecular Biology 2022Quote: ... by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore®; Merck). Details of primary antibodies used are provided in Supplementary Table 2 ...
-
bioRxiv - Immunology 2024Quote: ... 70 μL of alkaline phosphatase substrate (5 mM p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and color was allowed to fully develop ...
-
Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
-
bioRxiv - Immunology 2024Quote: ... 70 μL of alkaline phosphatase substrate (5 M p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and plates were incubated at room temperature to allow color development ...