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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated via 4-15% SDS-PAGE electrophoresis and transferred to a PVDF membrane (IPVH 00010, Millipore). Membranes were immersed in blocking solution (5% nonfat milk in 0.1 % Tween 20 in 1X TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were separated by 10% SDS-PAGE and then transferred to polyvinylidene fluoride membranes (Millipore, USA). The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the gels were transferred onto an activated polyvinylidene difluoride (PVDF) membrane (Immobilon from Millipore, # IPFL00005) and then blocked with 1X TBS-0.1% Tween-20-5% dry milk for 1 hour at room temperature on a rocker ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred to a 0.45 μm Immobilon-FL PVDF membranes (IPFL00010; EMD Millipore, Burlington, MA, USA) using the eBlot™ L1 Fast Wet Transfer System (GenScript ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The RIG-I protein fractions were pooled and concentrated using a 30 kDa Amicon Ultra filter (Millipore #UFC903008). Lastly ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribonucleoprotein (RNP) complexes (dCas9-gRNA) were formed by mixing the annealed gRNA constructs with dCas9 protein (Sigma-Aldrich) in 2:1 molar ratio (600 nM ...
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... appropriate AGO:RNA bands were excised from the gel and protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chloroform phase separation ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Immunology 2023Quote: ... The eluted protein was concentrated to 500 μl with a 100-kDa molecular mass cut-off concentrator (Millipore). Concentrated apo-KSHV-GPCR-Gi complex was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Biophysics 2024Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, C4706) at 40°C for 30 min and alkylated with 20 mM Iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
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bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 30-100 μg of protein were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Protran, Sigma-Aldrich). Membranes were blocked in PBS or TBS containing 3% BSA or 5% skimmed milk ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was dialysed against water then concentrated using an Amicon Ultra-Ultracel 5 kDa centrifugal filter (Millipore). Antibodies were raised in two guinea pigs by Eurogentec.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were then separated on 12% SDS PAGE and transferred to a PVDF membrane (Millipore Sigma, 0.2 μM). Membranes blocked with 5% milk were then probed with anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing desired protein were pooled and concentrated to 500 μL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then precipitated by addition of 800 µL of ice-cold 55% trichloroacetic acid (TCA) (Sigma Aldrich). Samples were left on ice for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). After the nonspecific binding sites had been blocked ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore, Darmstadt ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sized proteins were concentrated to >50 mg mL-1 using 10 kDa molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Systems Biology 2023Quote: Well plates containing single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore, IPVH00010). The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on denaturing polyacramide gels before transfer to a 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... the eluted proteins were concentrated in an Amicon®Ultra-15 Centrifugal Filter Unit (Millipore; 10 kDa cutoff) and incubated with the SUMO protease Ulp1 (Ulp1:His-SUMO-tH2 = 1:500 w/w ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were extracted from 400,000 to 500,000 cells with RIPA buffer with the addition of proteinase inhibitor (Sigma) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-glial fibrillary acidic protein (GFAP) antibody (dilution; 1:100, Sigma-Aldrich (G6171), St ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore Sigma) by semi-dry transfer ...