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Citations for Millipore Sigma :
8401 - 8450 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and protease inhibitor mixture] and IB assays were performed as previously described (39) using antibody against either FLAG (Sigma–Aldrich), HIF-1α ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were centrifuged 15 min at 13200 rpm at 4°C and protein concentration measured by Bradford assay (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: Antibiotic sensitivity assays were performed in cation-adjusted (CaCl2 10 mM, MgCl2 5 mM) Mueller-Hinton (pH 7.4) medium (CAMH, Sigma-Aldrich). Overnight cultures of A ...
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bioRxiv - Cell Biology 2021Quote: ... Osteogenic differentiation was analyzed by alkaline phosphatase (ALP) staining with Fast Blue assay (naphthol-AS-MSC phosphate and Fast Blue RR, Sigma) in Tris-HCl buffer (pH 8.9 ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Cell Biology 2019Quote: ... and then 50 ul of the preincubated mixture was transferred to the assay plates after addition of 50 ul of actinomycin D at a final concentration of 1ug/ml (Sigma). Incubation of the assay plate was continued for 18 h followed by addition of 20 μl of a MTT Reagent (R&D) ...
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bioRxiv - Immunology 2020Quote: ... Respiration profile was assessed in 96XF instrument with Mitostress assay as indicated upon cell treatment with oligomycin (#75351, 2µM, Sigma-Aldrich), FCCP (#C2990 ...
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bioRxiv - Cell Biology 2021Quote: ... The debris were removed from the lysates by centrifugation and protein estimation was performed by Bicinchoninic Acid (BCA) assay (Sigma). Proteins were resolved by loading 10ug of protein on a 10% and 12% SDS-PAGE gel ...
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bioRxiv - Physiology 2020Quote: ... Final triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2020Quote: Proximity ligation assay was performed with Duolink® In Situ Orange PLA reagents according to the manufacturer’s protocol (Sigma Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... activity embryos were homogenized in an assay buffer composed by KH2PO4 (25 mM, pH 7.4) and MgCl2 (5 mM) containing protease and phosphatase inhibitors (Sigma Aldrich). Total lysates were then quantified using a BCA Protein Assay Kit (Thermo-Scientific) ...
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bioRxiv - Immunology 2022Quote: ... the culture supernatants were collected and Luminex assay was performed according to the manufacturer’s protocol (EMD Millipore, Cat# MCYTOMAG-70K).
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bioRxiv - Cell Biology 2022Quote: Proliferation of UM51-PrePodo cells grown in Proliferation Medium and Advanced RPMI was measured with a resazurin assay (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... assays were performed as previously described (Bruno T, Cancer Cell, 2002) by using anti-acetyl-Histone H3 (Lys27) antibody (Millipore). Equal amounts of precleared chromatin were added to antibody-bound Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
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bioRxiv - Systems Biology 2020Quote: ... Cardiovascular risk markers in plasma were measured using MILLIPLEX MAP Human Cardiovascular Disease (CVD) Magnetic Bead Panel (1 to 4) - Cardiovascular Disease Multiplex Assay (HCVD1MAG-67K, HCVD2MAG-67K, HCVD3MAG-67K, HCVD4MAG-67K, Millipore). Experiments were performed by the Stanford Human Immune Monitoring Center (HIMC ...
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bioRxiv - Physiology 2019Quote: ... and neuropeptide serum levels were measured by Luminex assay according to the manufacturer’s instructions (#MMHMAG-44K, #MPTMAG-49K, and #RMNPMAG-83K from Millipore Sigma). Serum insulin and testosterone levels were measured using ELISA kit purchased from Millipore (#EZRMI-13K ...
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bioRxiv - Plant Biology 2019Quote: ... was assessed by evaluating the cell viability by MTT assay using 3-(4,5-dim-ethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide reagent or MTT (Sigma-Aldrich®) [36] ...
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bioRxiv - Microbiology 2019Quote: The caudal quarter of the flash frozen brain tissue was homogenized in radioimmunoprecipitation assay buffer (1% Triton-X, 0.1% SDS, 1X PBS) and 1% phosphatase inhibitor cocktail (P5726-1ML, Sigma-Aldrich) and 1% protease inhibitor cocktail (P8340-1ML ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and AChE activity was measured using the standard Ellman assay (Ellman et al., 1961) with 5,5’-Dithiobis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine iodide (ASChI) as substrates (Sigma-Aldrich). All plates contained three blanks with DTNB ...
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bioRxiv - Immunology 2020Quote: ... and 3×105 PBMC per well were seeded with 40 μl assay medium in XF 96-well cell culture microplate coated with poly-D-lysine (Sigma). The plate was centrifuged at 200 x g for 1 sec with no brake ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Developmental Biology 2020Quote: HLECs were treated with chemicals as described above and their intracellular permeability was analyzed using In Vitro Vascular Permeability Imaging Assay (Cat# 17-10398, Sigma) according to manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: ... The clean pellet was resuspended in 0.5–1 ml MAITE+BSA-FFA buffer and the concentration was quantified using the Bradford assay (Sigma-Aldrich). Mitochondria were stored at -80°C in MAITE+BSA-FFA buffer or centrifuged for 10 min at 10000 × g and 4°C and subsequently resuspended in the buffer required for the following analysis ...
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bioRxiv - Physiology 2019Quote: ... the lead acetate/lead sulfide assay is setup by initially preparing the reaction mixture of 10mM L-cysteine (Sigma #168149) and 1mM pyridoxal phosphate (PLP ...
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bioRxiv - Plant Biology 2019Quote: ... Then 25 uL of a dilution 1:10 were used to determine inorganic phosphate concentrations using the molybdate phosphate assay (Sigma), following kit instructions ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Cell Biology 2019Quote: The viability of cells was determined using the standard MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (MTT, Sigma-Aldrich, PL). The cells were incubated on 96-well plate (Perkin Merkel ...
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bioRxiv - Microbiology 2021Quote: ... Sensitivity to micafungin was measured at least in triplicate in a polymerization assay using a 96-well multiscreen high-throughput screen filtration system (Millipore) with a final volume of 100 µl ...
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bioRxiv - Physiology 2021Quote: ... an isotonic solution containing luciferase-luciferin (LL) was prepared from a vial of ATP assay mix-lyophilized powder (Sigma-Aldrich) was used ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Female mice were primed subcutaneously 48 hours before the assay with 0,1ml estrogen (1mg/ml, Sigma E815 in sesame oil) and 4 hours before the assay with 0,1ml progesterone (5mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration of cell lysates was measured using Bradford assay and equal amount of protein was added to anti-flag M2 magnetic beads (Sigma) in the presence of RNaseA (50 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Cytokines and chemokines in the homogenates were quantified using Luminex technology with a MILLIPLEX Map Mouse Cytokine/Chemokine Magnetic Bead Panel 25-plex Immunology Multiplex Assay (Cat. No. MCYTOMAG-70K-PMX, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged at 10,000 g for 5 minutes and total protein in the supernatant was measured using the bicinchoninic acid (BCA) assay (Sigma). Primary antibodies were detected using a secondary antibody conjugated to horseradish peroxidase ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability in response to drugs in 2D monolayer cultures was quantified using an XTT assay according to the manufacturer’s protocol (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Growth assay for inulin as carbon source was carried out by adding 20 µl of 20% inulin (Inulin from chicory, Sigma) or 20% D-Glucose as positive control into 1 ml minimal medium ...
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bioRxiv - Immunology 2021Quote: ... Transfected cells were then washed with phosphate-buffered saline (PBS) and lysed with radioimmunoprecipitation assay buffer (RIPA buffer) (Sigma, Germany). The harvested cell lysates were subjected to western blot analysis to verify the expression of S protein using in house mouse anti-S (SARS-CoV-2 ...
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bioRxiv - Immunology 2020Quote: For the proliferation assay splenocytes were plated in a Corning Costar 96-well round bottom cell culture plate (Sigma-Aldrich) at a cell density of 2.5 × 105 cells in a final volume of 170 µL ...
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bioRxiv - Immunology 2021Quote: Cytotoxicity of the isolated PAC used for the stimulation of murine macrophages was assessed using neutral red assays (Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Cell Biology 2021Quote: ... Extraction of RNA from bead sprout assays (described below) using TRIzol was performed after the removal of HDFs with trypsin-EDTA Solution 10X (59418C, Sigma). cDNA was synthesized with iScript Reverse Transcription Supermix (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration for the soluble fraction was adjusted (3µg/ml) following bicinchoninic acid assay measurement (BCA, Sigma-Aldrich, Poole, UK) and dilution in lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: In vitro histone acetylation assays were performed on 25 μg of mitotic chromatin (isolated as described above and quantified using the bicinchoninic acid colorimetric assay system (Sigma)) in 50 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... HAT activity was quantified by: i) a colorimetric assay quantifying the release of Co-A using DTNB (2-nitrobenzoic acid) (Sigma) by measuring the absorbance at 412nm ...
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Microbiology 2022Quote: Total protein was extracted from LT toxin-treated (100ng/ml) human differentiated enteroid monolayers (235D line) and untreated control using radioimmunoprecipitation assay (RIPA) buffer (Sigma) containing protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... the water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/mL horseradish peroxidase [Millipore-Sigma] with or without immune elicitors) ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: Enzymatic Assays from skeletal muscle tissues were done from muscle lysates prepared in the assay buffer as per the manufacturer’s protocol (Sigma-Aldrich). Serum calcium levels were measured using calcium estimation kit (Abcam #ab102505 ...