Labshake search
Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... solution containing 167 mg/ml of Gd-DTPA Diethylenetriaminepentaacetic acid gadolinium(III) dihydrogen salt hydrate (Gd; Sigma-Aldrich, Rehovot, Israel) to obtain a final lipid concentration of 50 mM ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration for the soluble fraction was adjusted (3µg/ml) following bicinchoninic acid assay measurement (BCA, Sigma-Aldrich, Poole, UK) and dilution in lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were placed in a 2.0 mL BeadBug pre-filled microcentrifuge tube on ice containing 1.0 mm acid washed silica beads (Sigma, Burlington, MA) and 1 mL of total lysis buffer solution ...
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bioRxiv - Neuroscience 2022Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to imaging except where noted ...
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bioRxiv - Developmental Biology 2022Quote: ... Around 1000 mESCs were suspended in 20 µl of differentiation medium (mESCs media without LIF, PD0325901 and CHIR99021, supplemented with 1µM retinoic acid (Sigma-Aldrich). After 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... and beaten on a bead-beater for 5 minutes at room temperature with 100 µL of acid-washed glass beads (cat. no. G8772, Sigma). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Fatty acids were then methylated with 2 mL of Boron Trifluoride (BF3) in Methanol (14% W/V) (Sigma Aldrich, USA) and incubated at 55 °C for 1.5 hours with intermittent shaking every 20 min ...
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bioRxiv - Cell Biology 2022Quote: In vitro histone acetylation assays were performed on 25 μg of mitotic chromatin (isolated as described above and quantified using the bicinchoninic acid colorimetric assay system (Sigma)) in 50 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... HAT activity was quantified by: i) a colorimetric assay quantifying the release of Co-A using DTNB (2-nitrobenzoic acid) (Sigma) by measuring the absorbance at 412nm ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were thereafter concentrated by a centrifugal evaporator at 60°C until complete drying (approximately 2h) and resuspended in 30 μL of 0.1% trifluoroacetic acid (TFA) buffer (Sigma-Aldrich). Peptides were purified with ZipTip tips containing a C18 column (EMD Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... or TAP-HA-YTM1 RPL3-Flag fusions under control of the respective endogenous promoters were grown in YPD or SDC-Leu medium and as indicated in the figures incubated in the presence of 0.5 mM auxin (3-indoleacetic acid, Sigma-Aldrich) for 120 min prior to harvesting the cells ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were acidified to 0.67% trifluoroacetic acid (TFA) and then desalted using C18ZipTip (Millipore cat no. ZTC18 5096). Dried peptides were frozen ...
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Bioengineering 2022Quote: ... the EBs were transferred to the spinal cord medium I (ScM I) supplied with 10 nM retinoic acid (Sigma Aldrich) and 3 μM CHIR-99021 (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... according to methods previously published.[28,29] Microcapsules were constituted of 1.0% w/v alginic acid (PH163S2, JRS Pharma GmbH, Germany) and 1.0% w/v gelatin type A (Sigma-Aldrich, Germany). These polymers were dissolved in a solution containing 0.1M HEPES (pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... PRP is washed once in 10% acid-citrate dextrose buffer (85 mM Na3-citrate, 71 mM citric acid, 111 mM glucose) with 111 µM prostaglandin E1 (Sigma), twice in washing buffer (36 mM citric acid ...
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bioRxiv - Microbiology 2022Quote: ... transfected parasites were selected in HFFs for integration of the plasmid into the genome with 25 μg/mL mycophenolic acid (Sigma) and 50 μg/mL xanthine (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence of GRP7 or its mutated variants plus the GFP tag connected by a short linker (7 amino acids [aa]) or GFP alone were cloned into the EcoRI and BamHI restriction site of the pET32a expression vector (Novagen) containing a thioredoxin (Trx ...
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bioRxiv - Microbiology 2022Quote: ... Yeast were grown on 2% (w/v) agar plates containing yeast nitrogen base (YNB) without amino acids (6.8 g/l, Sigma-Aldrich) supplemented with yeast synthetic drop out (YSDO ...
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bioRxiv - Immunology 2022Quote: The excipients D-Sorbitol and L-glutamic acid monosodium salt monohydrate (MSG) were purchased from Sigma Aldrich (St. Louis, MO), and magnesium chloride hexahydrate (MgCl2.6H2O ...
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bioRxiv - Microbiology 2022Quote: ... the methanol:water solution was evaporated under a N2 stream and a 125 µM stock solution was prepared in 4 mg/mL fatty acid free Bovine Serum Albumin (Sigma) in PBS and stored at -20 ºC for up to 3 months.
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bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Auxin-induced degradation of Ring1b in AID::Ring1b;FUCCI was carried by adding water-dissolved auxin (IAA, Indole-3-acetic acid sodium salt, Sigma) to cell media to a final concentration of 500 µM as described previously 46 ...
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bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described (Crouch et al. ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in ACSF containing membrane-permeant AM variant of the fast exogenous Ca2+ chelator 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM, 10 µM, Sigma-Aldrich) for 30 min and then returned to normal ACSF for another 30 min before recording ...
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bioRxiv - Molecular Biology 2022Quote: Freshly-seeded healthy antral undifferentiated mucosoid cultures were treated every 24 h with 4 µM 5aza (in 0.02 % acetic acid; Sigma-Aldrich). A global decrease in DNA methylation ...
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bioRxiv - Neuroscience 2022Quote: ... we added to the remaining lysate 350 µL of methanol and 10 μL of an internal standard mixture containing 50 µg/mL of 13C5-glutamine (Eurisotop) and 13C5-glutamic acid (Sigma). Following a 1.5 hour incubation on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... yejASm lacking the fragment encoding the first 30 amino acids (signal peptide) was PCR-amplified from Sm1021 genomic DNA and cloned into a pET29b(+) vector (Novagen) generating a C-terminal 6His-tag ...
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bioRxiv - Neuroscience 2022Quote: Cell survival was measured by tile imaging direct fluorescence of the VAChT-tdTomato reporter in iCrMNs and iSpMNs differentiated in a 96 well plate and treated with cyclopiazonic acid (CPA, C1530-10MG, Sigma) on differentiation day 10 ...
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bioRxiv - Molecular Biology 2022Quote: ... where individual amino acids concentrations are as defined in Yeast Synthetic Drop-out Media Supplements (Sigma-Aldrich, Saint Louis, Missouri), and 2% Glucose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2022Quote: ... Dried samples were then resuspended in 150 µL methanol containing internal standards (15 µM U-13C, 15N amino acid mix, 5 µg/mL 13C2, 15N3 cytosine (Sigma), 10 µg/mL U-13C mannitol and trehalose (Omicron) ...
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bioRxiv - Microbiology 2022Quote: ... free fatty acid (C8 and longer) and glycerol concentrations were assessed using commercially available colorimetric kits (MAK044 and MAK117, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast strains were grown in synthetic dropout (SD) media containing Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... Polar metabolites were resuspended in 170 µL of 100% methanol containing 13C-15N labeled amino acids (30 µM, 767964, Sigma). UHPLC normal phase chromatography was performed using an Agilent 1290 LC stack ...
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bioRxiv - Developmental Biology 2022Quote: ... 12-well plates at an approximate cell density of 40,000 cells/cm2 in cSFDM with 2 μM Retinoic acid (Sigma, #R2625). From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... was applied at a concentration of 50 ng/g bw (T(50)) or 400 ng/g bw (T(400)) while Ditpa (3,5-diiodo-thyropropionic acid; Sigma-Aldrich) was given at a dose of 400 ng/g bw (D(400) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were routinely grown in LB medium and MOPS/MES minimal medium supplemented with 0.01% casamino acids (CAA) (Sigma-Aldrich), 4 g/L glucose ...
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bioRxiv - Bioengineering 2024Quote: ... Solvents A and B were composed of 50 mM formic acid adjusted to 4.4 pH with ammonia solution and acetonitrile (Sigma-Aldrich Acetonitrile E CHROMASOLV for HPLC ...
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bioRxiv - Biochemistry 2024Quote: ... 600 μL MQ and added to a 2 mL Eppendorf tube containing 0.5 mL acid washed glass beads (Sigma Aldrich). Cells were disrupted by bead bashing for 5 min using a Vortex Genie II with a Turbomix attachment (Scientific Industries) ...
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bioRxiv - Molecular Biology 2023Quote: ... Log-phase yeast cells (20-25 x 107) were harvested and washed once with phosphate-buffered saline (PBS) and once with 5% tricholoroacetic acid (TCA, Sigma) prior to storing at -80°C ...
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bioRxiv - Bioengineering 2023Quote: ... The gel was re-dispersed in the BaTiO3 shell precursor solution which was prepared by mixing 0.5 g citric acid (Sigma Aldrich) and 24 µL titanium isopropoxide (Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Physiology 2024Quote: ... LratCre+Cc1fl/fl donor cells were washed twice and incubated in phenol red-free DMEM before treating with 500µM nicotinic acid (NA) (Sigma-Aldrich) or buffer alone for 24hrs ...
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bioRxiv - Neuroscience 2024Quote: ... For the LC-MS analysis of amino acids 50 µL of the available 150 µL extract were mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to confocal imaging except where noted ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... loaded with 2 μM of Fura-2 AM (F1221, ThermoFischer Scientific), 0.1 % pluronic acid (Pluronic F-127, P3000MP, ThermoFischer Scientific) and 40 µM of BTS (203895, Sigma-Aldrich) in the dark at room temperature for 60 min ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were then dried in a vacuum centrifuge before being dissolved in a solution containing 2 % (v/v) acetonitrile and 0.1% (v/v) trifluoroacetic acid (Sigma-Aldrich) for subsequent LC-MS/MS analysis ...