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Citations for Millipore Sigma :
8501 - 8550 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... a binary gradient was used, with mobile phase A consisting of water (Tedia, Fairfield, Ohio, USA) and 0.1% formic acid (Sigma-Aldrich), and mobile phase B consisting of acetonitrile (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Then 1 × 107 cells were washed with sterile water and transferred to nitrogen starvation conditions [6.7 g/L YNB without amino acids and without ammonium sulfate (Sigma-Aldrich) and 20 g/l glucose] and grown at 30 °C and 200 rpm ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Microbiology 2020Quote: ... The supplemented sugars (glucose, fructose, galactose, mannitol, mannose, arabinose, rhamnose, glucuronic acid, and xylose) were purchased from Sigma-Aldrich (Israel). Nile Blue (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cell cultures were diluted in 5 ml of M9 with 0.4% glucose and 0.08% RPMI 1640 amino acids (Sigma-Aldrich R7131), and incubated in 37°C (strain EL2504) ...
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bioRxiv - Microbiology 2021Quote: ... and high (HGHM, >95%) methylated homogalacturonan were provided from Elicityl Oligotech whereas oligogalacturonides and D-galacturonic acid were provided from Sigma.
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bioRxiv - Genetics 2020Quote: ... Bead-beating was done in an Axygen 1.1 ml plate (P-DW-11-C-S) with 250 μl of acid washed 425–600 μm beads (Sigma G8722) per well ...
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bioRxiv - Plant Biology 2020Quote: ... with or without 5 μl of the PAT blocker 2,3,5-triiodobenzoic acid (TIBA, 1.25 mM final concentration) (Sigma-Aldrich, Rehovot, Israel). This agar cube ("donor agar" ...
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bioRxiv - Zoology 2020Quote: ... Summed spectra consisting of 200–400 individual spectra were acquired in positive ion reflectron mode using 5 mg/ml 2,5-dihydroxybenzoic acid (Sigma-Aldrich) and 5 mg/ml methylendiphosphonic acid (Fluka ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and AChE activity was measured using the standard Ellman assay (Ellman et al., 1961) with 5,5’-Dithiobis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine iodide (ASChI) as substrates (Sigma-Aldrich). All plates contained three blanks with DTNB ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µl of supernatant mixed with 190 µl of deionized water and added to 1 ml of normalized 10% (v/v) Acetic acid/90% (v/v) methanol/0,05% (w/v) Amido Black (Naphtol Blue Black, Sigma N3393) buffer ...
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bioRxiv - Immunology 2020Quote: ... at a density of 50,000 cells/well for indicated time were fixed in 2.5% Glutaraldehyde and Tartrate-Resistant Acid Phosphatase in osteoclasts were stained using TRAP staining kit according to manufacturer’s instruction (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... To visualize protein bands for excising and mass spectrometry processing, gels were stained with Coomassie (40% methanol, 20% acetic acid, and 0.1% Brilliant Blue R (B7920, Sigma-Aldrich)) ...
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bioRxiv - Physiology 2019Quote: ... Clean minced tissue was transferred to a manual dounce tissue grinder containing homogenization medium A supplemented with fatty acid-free 0.1% bovine serum albumin (BSA-FFA) (Sigma A7030). Occasionally ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
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bioRxiv - Genomics 2021Quote: ... Pt black (PtB) was electroplated on the Pt electrode array using a precursor of 0.08 wt% chloroplatinic acid (H2PtCl6) solution (Sigma-Aldrich) in H2O ...
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bioRxiv - Genomics 2021Quote: ... Expression of the Dam-POI constructs was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148). Lines were tested routinely for mycoplasma.
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... An aliquot of homogenate was used for bicinchonic-acid protein-level determination using commercial components (Sigma, #B9643, #C2284, and #P0914). Glycogen content was assayed by hydrolyzing glycogen into glucose with amyloglucosidase (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... and mobile phases A and B consisted of water (Tedia, Fairfield, USA) and 0.1% formic acid and acetonitrile (Sigma-Aldrich) and 0.1% formic acid ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with the corrected ΔA530 for treatment reactions being calculated as: Units of polygalacturonase in the filtrate were calculated via comparison to a standard curve of galacturonic acid (Sigma). Standards were made with between 50μl and 1 ml of monosaccharide solution (MS ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Auxin-inducible degron (AID)-tagged strains were constructed as described (Snyder et al., 2019) and freshly prepared 10,000x stocks (500 mM) 3-indole acetic acid (IAA) (Sigma Aldrich) dissolved in 100% ethanol were used to treat yeast cultures (YPD ...
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bioRxiv - Molecular Biology 2021Quote: ... Osteoclasts were stained for tartrate-resistant acid phosphatase (Acp5) using naphthol AS-MX and Fast Red TR salt (Sigma-Aldrich). The following primary measurements were made ...
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bioRxiv - Biochemistry 2021Quote: Chemicals and solvents (analytical grade) were purchased from the following sources: α-Cyano-4-hydroxycinnamic acid 98% (CHCA) (Sigma Aldrich), 2,5-Dihydroxybenzoic acid 98% (DHB ...
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bioRxiv - Biochemistry 2021Quote: ... 24h post-transfection LD formation was induced by adding fresh medium supplemented with 0.5 mM oleic acid (Sigma Aldrich, O1383) complexed with bovine serum albumin (Sigma Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... the membrane is blocked using 5% milk in TBS-T buffer (Tris-Buffered Saline pH 7.6 [TBS {20 mM Tris pH 8, 150 mM NaCl, Hydrochloric acid [Sigma Aldrich, 320331]}] with 0.1% Tween-20 [Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were normalised to an OD600nm of 0.1 in cation adjusted Mueller Hinton broth (MHB++) and 20 μl of resultant suspension used to inoculate 180 ul of fresh MHB++ containing either arachidonic acid (Sigma) or hydrogen peroxide (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... 30 min) for DNA (deoxyribonucleic acid) denaturation followed by 0.1M pH 8.5 sodium borate buffer washes (Sigma Aldrich, Gilligham, UK) (3×10 min) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged at 10,000 g for 5 minutes and total protein in the supernatant was measured using the bicinchoninic acid (BCA) assay (Sigma). Primary antibodies were detected using a secondary antibody conjugated to horseradish peroxidase ...
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bioRxiv - Cell Biology 2020Quote: ... NAD+ or NADH were extracted with an acidic solution (10% (mitochondria, mammalian cell, yeast) or 20% (fly) trichloroacetic acid (TCA) (Sigma) or basic solution (0.5 M sodium hydroxide (NaOH ...
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bioRxiv - Microbiology 2020Quote: ... Each sample was spotted onto a titanium dioxide-coated plate (0.15 μL/spot) and covered with 0.15 μL matrix mixture (a saturated solution of α-cyano-4-hydroxycinnamic acid (Sigma) in 50% acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... coli JKe201 [28] was cultured in the presence of 100 µM of diamino pimilic acid (DAP) (Sigma Aldrich D1377-10G). Salmonella enterica serovar Typhimurium SL1344 was cultured in LB in the presence of 90 µg/ml streptomycin (Sigma-Aldrich S9137-100G) ...
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bioRxiv - Microbiology 2020Quote: ... Dried samples were resuspended in 10 µL of 0.1% trifluoroacetic acid then purified by solid phase extraction (SPE) using C18 Ziptip (stage-tip; EMD Millipore) following the manufacture’s protocol ...
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bioRxiv - Immunology 2021Quote: ... HLA typed HLA-A*02:01+ PBMCs from COVID-19 recovered individuals were incubated with SARS-CoV-2 peptide pools (2µM / peptide) and cultured for 10-14 days in RPMI-1640 supplemented with 1x Non-essential amino acids (NEAA; Sigma), 5 mM HEPES (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... coli strains were cultivated at 37°C in Luria-Bertani medium with shaking at 37°C in the presence of 0.3 mM thymidine or diaminopimelic acid (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2021Quote: ... and human choriocarcinoma/trophoblastic cancer (JEG-3) cells were grown in DMEM supplemented with 10% FBS and MEM Non-essential Amino Acid Solution (Sigma). C6/36 cells were grown at 28°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... followed by single-colony picking and overnight culture in antibiotic-supplemented Luria broth (nalidixic acid, 50 μg/ml; N4382 Sigma and/or kanamycin ...
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bioRxiv - Microbiology 2021Quote: ... The gel was then washed with the fix/wash solution (25% ethanol, 10% acetic acid in water) and stained by 0.1% Alcian blue (Sigma-Aldrich) dissolved in the fix/wash solution for 15 min in the dark ...
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bioRxiv - Immunology 2021Quote: ... dermatitidis ATCC strain 26199 was used for this study and grown as yeast on Middlebrook 7H10 agar with oleic acid-albumin complex (Sigma) at □ LJC.
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bioRxiv - Immunology 2021Quote: ... 0.5 µl of an aqueous solution of 15–50 kDa poly(L-glutamic acid) (PGA, Sigma-Aldrich, 100 µg/µl) was mixed with 0.48 µl of synthetic modified sgRNA (2’-O-Methyl at 3 first and last bases ...
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bioRxiv - Microbiology 2021Quote: ... RH TIR1-3FLAG or RH TgPDE-mAID-3HA tachyzoites cultivated in HFFs in D10 medium were treated with 0.5 mM 3-indoleacetic acid (auxin; IAA) (Sigma Aldrich) prepared in 100% ethanol (Pharmco ...
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bioRxiv - Immunology 2020Quote: ... nigericin (N7143), tamoxifen (T5648), flufenamic acid (FFA, F9005), CP-456773 (MCC950, PZ0280), sodium iodide (NaI, 409286) were purchased from Sigma. Disuccinimidyl suberate (DSS ...
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bioRxiv - Immunology 2020Quote: ... dermatitidis ATCC strain 26199 was used for this study and grown as yeast on Middlebrook 7H10 agar with oleic acid-albumin complex (Sigma) at 39 °C.
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... cells were collected with 0.25% trypsin-EDTA and lysed in 4-morpholineethanesulfonic acid (MES)-buffered saline (MBS; 25 mM MES, 150 mM NaCl, pH 6.5) containing 500 mM sodium carbonate (Sigma, #S7795) and a protease inhibitor cocktail Set III (Calbiochem ...
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bioRxiv - Developmental Biology 2021Quote: ... The stained sections were rinsed in distilled water and staining outside of perikarya was differentiated for 10 min in 96% EtOH with 0.02% glacial acetic acid (Millipore Sigma). The sections were subsequently dehydrated 2x 5 min in 99% EtOH and 2x 5 min in Xylene prior to coverslip mounting using DPX (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Fine separation of SCFA was confirmed by the complete separation of the volatile-free acid mix (Sigma, Oakville, ON, Canada). Data are presented as absolute values (g of SCFA per g of feces).
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bioRxiv - Genetics 2020Quote: ... the URA3 gene was cured out by streaking out the selected colonies on SD medium containing (1g/l) 5-Fluoroorotic acid hydrate (Sigma) plates as described previously (Boeke et al. ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...