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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Biochemistry 2019Quote: The TAP tag was detected using Peroxidase-anti-peroxidase 1:5000 (Sigma, Lot #103M4822), Pgk1 was detected using mouse anti-Pgk1 1:7500 (459250 ...
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bioRxiv - Physiology 2023Quote: ... TAG levels were then measured using the Serum Triglyceride Determination Kit (TR0100-1kt, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... These antisense DNA probes were synthesized with Biotin-TAGs at the 5’ ends (Sigma) and 100 µM probes were pooled in equimolar ratios ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
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bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
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bioRxiv - Physiology 2023Quote: TAG and total cholesterol evaluation were processed according to the manufacturer’s instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... the GST tag was then removed by addition of 20μL thrombin (Millipore 69671-3), and the sample was cleaved at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated for 1 hour with HIS-Select® Nickel Affinity Gel (Sigma), 300 μL of gel per 500 mL of His6-SUMO-AGARP expression cell culture ...
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bioRxiv - Genetics 2019Quote: ... from HEK293 cells stably expressing p3XFLAG-ADCK4 or -BAP were incubated with 80 μL of FLAG M2 agarose beads (Sigma-Aldrich) for 48 h at 4°C in an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...
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bioRxiv - Cancer Biology 2023Quote: ... To pcw-KRAS-G12D-blast vector was packaged into a viral assembly by co-transfecting with PMD2.G (addgene, 12259) and PSPAX2 (addgene,12260) into HEK293 T cells using polyethyleneimine (PEI) transfection reagents (Sigma 408727). The virus-containing medium was used to infect HPNE E6/E7/st40 cells with 8ug/ml polybrene ...
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bioRxiv - Cancer Biology 2023Quote: ... Miapaca2, Hek293 were cultured with high-Glucose DMEM medium (Fisher, SH30423FS) supplemented with 10% FBS and 1% Pen-Strep (Sigma, 11074440001). The BxPC3 cell were cultured with RPMI1640 (Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...