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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... The C-terminal twin-Strep-Tag was removed by HRV3C (Sigma Aldrich) digestion overnight at 4 °C and the uncleaved protein was removed by passage over Ni-NTA resin ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were performed with anti-GFP tag primary monoclonal antibody (Sigma-Aldrich) as described by50.
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bioRxiv - Microbiology 2021Quote: ... and the antibody for the FLAG tag was from Sigma (cat # F7425).
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2022Quote: ... HA tag antibody (1:1200, cat# 04-902; EMD Millipore, clone DW2). Blots incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into PET-24a (+) C-terminal-histidine-tag vector (Novagen, Germany), which was digested by XhoI and NdeI and dephosphorylated by Antarctic Phosphatase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... but with removal of the His6 tag was by a thrombin (Sigma) protease cleavage step before concentration to 40 mg/ml.
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bioRxiv - Physiology 2020Quote: ... Ptch1 was immunoprecipitated with anti myc-tag antibodies (Millipore, Cat# 05-724). Expression of Dhh ...
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bioRxiv - Biochemistry 2021Quote: ... monodisperse tag-free Ape1 was concentrated using 10 kDa MWCO Amicon (Millipore) to 15–20 mg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Immunology 2021Quote: ... Antibodies to Anti-Flag-tag (F1804) for immunoprecipitation were obtained from SIGMA. Antibodies to Ub (ab7780) ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with an anti-HA epitope tag PAb (Sigma, H6908) and then with a FITC-conjugated donkey anti-rabbit secondary Ab (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
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bioRxiv - Plant Biology 2022Quote: ... Other primary antibodies we employed were: anti-HA tag antibody (Sigma, H6908); anti-c-Myc tag antibody (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... The following rabbit antibodies were used: HA-tag (Sigma-Aldrich H6908; RRID:AB_260070); LAMP1 (Cell Signalling D2D11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies for FLAG- tag (Cat # F3165, RRID:AB_259529) was obtained from Sigma-Aldrich. KDM2A (Cat # ab191387 ...
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bioRxiv - Cell Biology 2023Quote: ... Triacylglycerol (TAG, Cat# T7140) and monoacylglycerol (MAG, Cat# M2015) were from Sigma. β-Glucosylceramide (β-GlcCer ...
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bioRxiv - Biophysics 2023Quote: ... containing a C-terminal EmeraldFP–6xHIS tag was cloned into pET22b (Novagen) for expression in E ...
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bioRxiv - Biochemistry 2023Quote: ... S-tag was removed with Factor Xa Cleavage Capture kit (Millipore Sigma). The untagged sF or sG was further purified with size-exclusion column (HiLoad 16/60 Superdex 200 prep grade XK 16 gel filtration column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-myc tag (Sigma-Aldrich, M4439; IB, 1:10,000; IP, 1 μg;), anti-AP3B1 (Bethyl labs 50-157-0195 ...
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bioRxiv - Microbiology 2023Quote: ... and Trx tags derived from the pET-32a/pET28 cloning vector (Novagen) from ClearColi (Lucigen ...
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bioRxiv - Cell Biology 2024Quote: ... Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). Rabbit anti-K8.1 (gift of Denise Whitby ...
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bioRxiv - Cell Biology 2024Quote: ... PLK1 rabbit (used in Phos-tag gel and PLK1 IP, Millipore ABE2619), phospho-PRC1 T481 (Abcam ab62366) ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...