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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Biophysics 2020Quote: ... Thrombin cleavage of the affinity tag was performed using the Sigma Thrombin CleanCleave Kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with the antibodies as follows: antibodies for HSV tag (Millipore, 69171), SREBP-1 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2019Quote: Selected oligonucleotides were ordered with a 5′-biotin tag and HPLC-purified from Sigma-Aldrich. Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8 ...
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bioRxiv - Physiology 2019Quote: ... flag epitope tag (Cell Signaling clone D6W5B, 14793S and Sigma clone M2, F3165 and A2220), GST (Cell Signaling clone 91G1 ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
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bioRxiv - Biophysics 2020Quote: ... FLAG and T7 affinity tags were detected with anti-FLAG (F3165, M2 clone, Sigma-Aldrich) and anti-T7 antibody (69522 ...
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bioRxiv - Microbiology 2020Quote: ... Wild-type and mutant VP40 bearing a Strep-Tag were cloned into pTriEx-5 (Novagen) and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary anti-Flag tag antibody was obtained from Sigma-Aldrich (F7425-.2MG, WB 1:2000). Primary antibodies against viral proteins were obtained from ...
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bioRxiv - Microbiology 2022Quote: ... the primary antibodies were horse radish peroxidase-conjugated α-poly-histidine tag antibody (Sigma Aldrich; St ...
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bioRxiv - Developmental Biology 2019Quote: ... and the histidine tag removed with carboxypeptidase A bound to agarose (Sigma, St. Louis, MO) at a ratio of 10 protein:1 enzyme (w/w) ...
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bioRxiv - Immunology 2023Quote: ... 68] (dilution 1:1000) and mouse mAb to FLAG tag (Sigma, F3165, dilution 1:500).
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bioRxiv - Molecular Biology 2023Quote: ... the sequence containing the HA tag were transferred in a bacterial expression vector pET22b (Novagen) between Xho1 and HindIII restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&Tag analysis was assayed for the following epigenetic markers: H3K4Me2 (Millipore, Cat# 07-030), H3K27Ac (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... the histidine tag was then removed by proteolytic digestion with 1U/mg of thrombin (SIGMA). The digestion mixture was then loaded again on the IMAC on a Ni2+-His-Trap column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with a rabbit anti-HA-tag antibody (H6908, Sigma‒Aldrich) in PBS-Tx supplemented with 0.2% BSA ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with M2 antibody (anti-FLAG-tag, catalogue no. F1804A-5MG; Sigma) at 4 ℃ for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Flag-tag (cat. 2368, 1:1000, Cell Signaling Technology and cat. F9291, 1:1000, Sigma), HA-tag (cat ...
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bioRxiv - Genetics 2024Quote: ... Western blots were reacted with monoclonal anti-S-tag (1:5,000; SAB2702227, Sigma-Aldrich®) as primary antibody and with polyclonal anti-mouse IgG peroxidase (1:5,000 ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Biochemistry 2024Quote: ... along with packaging vectors psPAX2 (#12260) and PMD2.G (#12259) were transfected into the HEK293-FT cells using XTremeGene 9 (Sigma Aldrich, 6365779001) (A375m ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag antibody (1:1000 dilution used) raised in mouse (F1804) was obtained from Sigma-Aldrich and Porin antibody (1:5000 dilution used ...
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bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
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bioRxiv - Microbiology 2019Quote: ... The hexa-histidine tag was removed overnight with thrombin (5 U thrombin (Sigma-Aldrich, Darmstadt, Germany) per mg SiiA protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Biochemistry 2021Quote: Monoclonal antibodies against the Flag tag epitope and Cdh1 were purchased from Sigma (St. Louis, MO) while antibodies against GFP ...
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bioRxiv - Biophysics 2020Quote: ... MSP1D1 was purified using IMAC72 with further cleavage of 6xHis-tag by TEV protease (Sigma-Aldrich). The lipid mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Molecular Biology 2022Quote: Full-length REC114 and its variants were cloned as Strep-tag fusions into pRSFDeut-1 (Novagen). TOPOVIBL 1-385 was cloned as a 6xHis-SUMO fusion protein in pETM11 ...