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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (A3854, Sigma-Aldrich) and imaged as above.
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... were separated by SDS-PAGE and protein was transferred to MeOH-activated Immobilon®-P membrane (Merck Millipore) using Whatman paper and a transfer buffer of 48 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: Protein expression via the metallothionine promoter was induced by supplementing the growth medium with 0.25 mM CuSO4 (Sigma). For BioID experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... The eluted protein was concentrated using ultrafiltration units with 10 kDa regenerated cellulose membrane (Amicon Ultra, Merck Millipore) and subsequently loaded on an equilibrated (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB western blot was performed by transferring proteins to Immobilon-P membranes (Millipore Biosciences, Temecula, CA) and blocked with 5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Microbiology 2021Quote: ... LACV-LCItag_H34K protein was finally loaded on a pre-equilibrated Superdex 200 Increase 10/300 GL (Sigma-Aldrich) size exclusion chromatography column in 50 mM Tris–HCl pH 8 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrillary acid protein (GFAP) (1/1,000; Sigma-Aldrich, St. Louis, MO, USA; Cat. No. G3893); mouse anti-S100β (1/1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then eluted with lysis buffer 5 supplemented with 20 mM glutathione (Sigma Aldrich, product # G4521) and the elution fractions were pooled together and dialyzed into dialysis buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 μg of protein from each group was incubated with 50 μL of M2 Flag Agarose beads (Sigma) overnight with gentle rocking at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated by 3–15% SDS-PAGE and transferred to polyvinylidene fluoride-FL (PVDF-FL, Millipore Sigma) membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins (0.5-1 mg) were precipitated using the anti-FLAG antibody (1:100, Cat. #F7425; Sigma-Aldrich), anti-HA antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Half fresh medium containing Amyloid β Protein Fragment 1-42 (50 nM; Sigma-Aldrich, St. Louis, MO, USA) or DMSO (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was quantified with the bicinchoninic acid (BCA) method (Sigma, Santa Clara, CA, USA, Cat#BCA1-1KT). The extract was eluted with 2× sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were buffer exchanged using Amicon® Ultra-15 Centrifugal Filter Units (Millipore-Sigma, Oakville, Canada, Cat#UFC905024) with PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were buffer exchanged using Amicon® Ultra-15 Centrifugal Filter Units (Millipore-Sigma, Oakville, Canada, Cat#UFC905024) with PBS ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein per each sample were resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). The membrane was probed with rabbit monoclonal antibodies that recognize CD63 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore). Membranes were blocked in 5% milk or bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with nsp9 buffer containing 0.1 mg/ml 3xFlag peptide.
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bioRxiv - Biochemistry 2020Quote: Thaw the protein and concentrate it to 5mg/ml in Amicon Ultra-15,10000Mr cut-off centrifugal concentrator (Millipore). The hanging drop vapor diffusion method was useful to gain crystal at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were electrophoretically separated on 4–12% SDS-PAGE gels and transferred to a PVDF membrane (Millipore, MA). Membranes were subsequently blocked with blocking buffer (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with Bradford assay (Sigma B6916) on a nanophotometer (IMPLEN) ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein A/G agarose (Millipore, 100 μL/IP). The chromatin samples were split in two and diluted with IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Microbiology 2022Quote: RIP assay and RNA pull down assay were performed using RIP RNA-Binding Protein Immunoprecipitation kit (Sigma, USA) and Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... samples (100 µl culture broth) were subjected to protein precipitation followed by phospholipid removal (HybridSPE, Merck/Sigma Aldrich). The internal standard E2-d5 (Chromsystems ...
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bioRxiv - Genetics 2022Quote: ... The protein concentration in the supernatant was measured by the spectrophotometric Bradford method (Sigma Chemical Co., St Louis). Ascospore proteins extraction ...
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bioRxiv - Cell Biology 2022Quote: Protein isolation was performed by lysis of the cells in RIPA buffer (Sigma-Aldrich, St. Louis, United States), supplemented with complete protease and phosphatase inhibtors (Roche ...
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bioRxiv - Microbiology 2022Quote: ... stained with REVERT total protein stain (Licor Biosciences) and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes and probed with 1:1,000 a-FLAG primary (Sigma-Aldrich, clone M2) and 1:5,000 a-mouse secondary (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... the protein was dialyzed overnight at 4° C (Cat. # D9527-100FT, 14 kDa MWCO cellulose membrane, Sigma Aldrich) into CP storage buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The instrument was calibrated using a protein standard mixture: β-amylase (Sigma-Aldrich, 56, 112 and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2019Quote: ... Animal caps were dissected at stage 11 and lysed in the presence of protein phosphatase inhibitor cocktails (Sigma). Western blots were stained with rabbit anti-Smad1 antibody (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... Samples were pre-cleared with 35 µl Protein A Agarose/Salmon Sperm DNA beads (Millipore, Temecula, CA, USA) at 4ºC for 30 min with continuous rotation ...
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bioRxiv - Physiology 2019Quote: ... Equal concentrations of protein from each sample were incubated with 40 μL of M2 agarose beads (Sigma, #A2220) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... All samples were then diluted with TE (1:1) and RNA and proteins were digested (RNAse, Sigma-Aldrich® ...
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bioRxiv - Microbiology 2019Quote: ... The solubilised protein (10 μl) was mixed with 290 μl of Quick Bradford reagent (Sigma Aldrich, NSW, Australia) and incubated at room temperature for 5 min 42 ...
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bioRxiv - Microbiology 2019Quote: ... The eluted proteins were visualized by Western Blotting with the following antibodies: anti-FLAG M2 from Sigma Aldrich, anti-GFP from Torrey Pines ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected by western blotting using either Anti-FLAG M2 monoclonal antibody-peroxidase conjugate (Sigma, A8592) at a dilution of 1:10000 ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... and resin-bound lipids were eluted from the beads with 50 µL of BugBuster protein extraction reagent (Millipore). The beads were again sedimented ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluent was concentrated to 500 µL using an Amicon centrifugal concentrator (50 kD cut-off, Millipore) and loaded onto a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing pure protein were pooled and concentrated in a 3,500 MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 6 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the first boosting ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s complete adjuvant (Sigma-Aldrich) for priming ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Identical amounts of crude extracts were incubated with prebound @AGO1 (5μg) PureProteome Protein A magnetic beads (30μL; Millipore) for 2 hours at 7 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...