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Citations for Millipore Sigma :
8601 - 8650 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1.5×105 Vero cells in 24-well plates were transfected with 0.5 µg of the indicated S-protein expression vector using the GeneJuice transfection reagent (Novagen) and syncytia formation monitored for 24-48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654; 0.21% L-methionine, Sigma #M9625 ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–6.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–5.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to 40 mg ml-1 with a centrifugal filter device (Millipore 50 kDa MW cutoff), and frozen until crystallization.
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was concentrated and buffer exchanged into 0.5 x TNG buffer using an Amicon ultrafiltration device (Millipore) containing a 10 kDa membrane.
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Bioengineering 2020Quote: ... single-polymer and IPN hydrogels were incubated with an HA binding protein derived from bovine nasal cartilage (EMD Millipore) as previously described by Suri & Schmidt [31] ...
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bioRxiv - Biochemistry 2021Quote: ... The rAQP4ct protein domain was concentrated and exchanged into buffers for biophysical analyses with an Amicon centrifugal filter (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... The protein sample was concentrated by ultrafiltration (Amicon Ultra-4 centrifugal filter devices, 50,000 MWCO; Millipore, Billerica, MA, USA), and the solution was changed to buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced by adding 5 mM DTT (45 min, 56 °C) and free cysteines alkylated with iodoacetamide (Sigma, Taufkirchen ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2021Quote: ... and the expression of the bait-protein was detected from the input sample with anti-FLAG antibody (Sigma Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes associated with p53 were resolved by SDS-PAGE and transferred onto a PVDF membrane (#IPVH00010, Millipore). The membrane was blocked with 3% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were eluted twice for 30 minutes each at 58°C with 150 mM dithiothreitol (DTT; D0632, Sigma-Aldrich), 1% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... membranes are blocked with 5% milk or BSA for phosphorylated proteins in TBS+0.2% Tween®20 (Sigma-Aldrich) for 1h and incubated 1h with primary antibody at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Values were normalized with respect to the total protein on each sample determined by the BCA method (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... The protein sample was incubated with taxol-stabilized microtubules (15 mg mL-1) and 1 mM AMP-PNP (Sigma) and the mixture overlaid on a glycerol cushion (BRB80 ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein post cleavage was injected into FPLC and run over two HisTrap FF Crude 1 mL columns (Sigma-Aldrich) with FPLC running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... NaCl was removed from the buffer and the protein was concentrated to 100 mg mL−1 using a 50,000 Da molecular mass cut-off concentrator (Millipore). The concentrated protein was incubated with a four-fold molar excess of dalbavancin at 4 °C for 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... 150 μg of protein from each sample were loaded onto a 10-kDa Amicon Ultra-0.5 centrifugal filter (Millipore). The protein mixture was washed three times by adding 200 μL of UA buffer (8 M Urea ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing the S protein were pooled and concentrated using 50 kDa MWCO Amicon centrifugal filter units (EMD Millipore). During concentration ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted for measuring the activity of GUS and luciferase (LUC) activity using 4-methylumbelliferyl glucuronide (Sigma) and luciferin (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated via gel electrophoresis on 7.5-12 % polyacrylamide gel and transferred to a PVDF membrane (Sigma Aldrich) using a semi-dry blotting system ...
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bioRxiv - Microbiology 2020Quote: ... the proteins in another gel were electrophoretically transferred to a methanol-activated polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA) and reacted with COVID-19 hospitalized patient sera (#12) ...
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bioRxiv - Microbiology 2020Quote: ... cell pellets were lysed with the M-PER Protein Extraction Reagent (Pierce) complemented with a protease inhibitor cocktail (Sigma) and a phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein extraction buffer (50 mM TrisHCl, pH 7.0, 1% w/v SDS, P9599 protease inhibitor cocktail, Sigma-Aldrich) was added to the powder ...
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bioRxiv - Synthetic Biology 2021Quote: ... Ll.LtrB intron and LtrA protein was amplified from the commercial plasmid pACD4K-C (TargeTron gene knockout system, Sigma-Aldrich) with primers pGIIintron_fwd and rev (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were captured by addition of 75 μL EZview Red Anti-c-Myc Affinity Gel beads (Sigma). Beads were collected with centrifugation and rinsed at least 3 times with wash buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of tissue was ground in liquid nitrogen and protein was precipitated by 10% TCA (Trichloroacetic acid, Sigma) in acetone and the resultant precipitate was pelleted by centrifuging at 13000 rpm for 5 min ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... Viral supernatant was transferred into a new sterile 50 mL tube and passed through a low protein binding 0.45 μm filter (SE1M003M00, Millipore) to exclude any cells still present in the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore). The membranes were blocked with 5% bovine serum albumin in 1XPBS with 0.5% Tween 20 (PBST ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred by electroblotting onto polyvinylidene difluoride membrane (Millipore). After blocking 1 h with 5% nonfat dry milk ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were reduced and alkylated after treatment with 10 mM DTT (Astral Scientific) and 20 mM chloroacetamide (Sigma). The gel was dehydrated using 100% ACN and rehydrated with digestion solution ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing each DNA pol of a high purity were pooled and concentrated using a 10K protein concentrator (Millipore). Concentrated proteins were preserved at −20 °C in the presence of 50% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Boston, USA). After blocking with 5% non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... 15 mL protein extracts were incubated in a 50 μL slurry of anti-FLAG M2 affinity gel (Sigma, A2220) and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... coli total protein extract were spotted within a pre-marked grid onto a 0.2 μm pore–size nitrocellulose membrane (Merk Millipore). The membrane was left to dry to fix the proteins for 30 min at room temperature ...