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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... which would allow controlled expression of FOXL2WT or FOXL2C134W protein by addition of Dox (Sigma, D9891). Replication-defective lentivirus containing the rtTA Advanced TET-ON was generated by co-transfection of pLVX-TET-ON ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... 60 μL (50% slurry) of Protein-RIPA-equilibrated FLAG-m2 beads (Sigma-Aldrich, Cat. No A2220) were added together with 1 μL of Benzonase (Merck-Millipore ...
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bioRxiv - Plant Biology 2021Quote: Proteins fractionated by gel electrophoresis were transferred to polyvinylidene difluoride membranes (PVDF) (Immobilon®-P, Millipore) using a semi-dry blotting system (Biorad ...
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bioRxiv - Genomics 2020Quote: ... except for the following modifications: immunoprecipitation was carried out using monoclonal antibodies against protein A (Sigma); the lysis buffer contained 10mg/ml heparin (sigma H7405) ...
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bioRxiv - Neuroscience 2020Quote: ... Following the estimation of protein concentration using the Quantipro BCA assay kit (Sigma-Alrich, United States), equal amounts of lysate were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel and then transferred onto polyvinylidene fluoride membranes ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Protein bands were detected using the Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, MA catalog# WBKLS0100) and images were acquired using the In Vivo FX PRO imaging system (Carestream ...
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bioRxiv - Cancer Biology 2020Quote: RNA-binding protein immunoprecipitation was run according to supplier recommendations (Millipore, Magna RIP Kit, 17-700). Beads were conjugated with 10 μg either anti-IMP3 (#1 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma) at an absorbance of 0.8–0.9 at 600 nm ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) for 20-24h at 20°C ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was determined using the Bradford assay (Millipore, Billerica, MA, USA). Each protein sample was electrophoresed on 10% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... The protein lysates were separated via SDS-PAGE and transferred to a polyvinylidene difluoride membrane (Millipore). The western blot was performed using anti-puromycin (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were reduced by adding up to 5 mM dithiothreitol (DTT, Sigma-Aldrich Cat. Nr. D0632) and incubating at 60 °C for 45 min followed by alkylation with 10 mM iodoacetamide (IAA ...
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bioRxiv - Immunology 2022Quote: ... The proteins from the gels were electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA). The membranes were blocked with 5% skim milk prepared in Tris-buffered saline (150 mM NaCl and 20 mM Tris–HCl ...
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bioRxiv - Immunology 2022Quote: ... Double-purified protein was concentrated with Amicon Ultra Centrifugal Filters according to manufacturer’s instructions (Sigma-Aldrich) and the concentration determined by a BCA assay using the Pierce BCA Protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Total cellular proteins were extracted by homogenization in radio-immunoprecipitation assay (RIPA) lysis buffer (Sigma-Aldrich) supplemented with the Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Genetics 2022Quote: ... hPSC-CMs were stained with sarcomreirc protein TNNT2 (Catalog #: HPA017888 Lot #: A91786, Sigma, dilution 1/200), secondary antibody Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins in the lysate were reduced with 20mM Tris(2-carboxyethyl)phosphine (TCEP) (Sigma-Aldrich) and alkylated with 55mM 2-Chloroacetamide (CAA ...
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bioRxiv - Physiology 2022Quote: ... protein G beads were coupled to the pre-pro-vasopressin antibody or normal rabbit serum (Sigma) at a 1:50 ratio for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and stained in-gel with SYPRO Ruby (#S4942, Sigma-Aldrich) as loading control ...
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bioRxiv - Molecular Biology 2019Quote: The CbAgo WT and DM proteins were expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50 μg ml-1 kanamycin and 34 μg ml-1 chloramphenicol until an OD600nm of 0.7 was reached ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were eluted using 2x Laemmli sample buffer with freshly added D-thiotreitol (200 mM, Sigma) for 10 min at 95°C.
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bioRxiv - Pathology 2019Quote: Serum proteins were separated by reducing SDS-PAGE (10%) and transferred onto polyvinylidene difluoride membranes (Millipore). Membranes were blocked in 5% milk Tris-buffered saline (TBS) ...
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bioRxiv - Plant Biology 2019Quote: ... proteins in fusion with 2xStrep-FLAG were purified with Anti-FLAG M2 Magnetic Beads (Sigma Co.). Purification was carried out according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Cell Biology 2019Quote: ... Silicon wafers were silanized for protein conjugation by incubation with 3-glycidoxy-propyl-dimethoxy-methylsilane (Sigma) (0.045 % in 100 % EtOH ...
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bioRxiv - Immunology 2019Quote: ... CD14 peridinin-chlorophyll protein complex (PerCP, clone TM1, DRFZ Berlin) and propidium iodide (PI, Sigma Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration of the membrane preparation was determined by the bicinchoninic acid assay kit (Sigma-Aldrich), the sample concentration was adjusted to of 3–10 mg/mL membranes were aliquoted (50 or100 μl) ...
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bioRxiv - Molecular Biology 2019Quote: RIP experiments were performed using a Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 uL of pre-washed magnetic beads (Magna ChIP Protein A+G Magnetic Beads, Millipore Sigma) were added to each sample for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Seven milligrams of protein was incubated with 40 μL α-FLAG M2 magnetic beads (Sigma M8823) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Protein extracts were subject to SDS-PAGE and were transferred to Immobilon-FL PVDF membrane (Millipore). Membranes were blocked for a minimum of 1-hour in Odyssey Blocking Buffer (OBB ...
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bioRxiv - Neuroscience 2019Quote: ... after which endogenous non-specific protein binding was blocked by incubation in 15% normal serum (Sigma) in TBS-T (TBS with 0.3% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted using 100 μL of FLAG peptide (100 μg/mL, Sigma-Aldrich, F4799), and then with 100 μL of 2x Laemli buffer (62.5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absolute quantification of each protein is performed through applying AQUA™ Peptides purchased from Sigma-Aldrich. A summary of all quantification results were shown in S8 Table.
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bioRxiv - Physiology 2019Quote: ... after which the proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Millipore, Billerica, MA, USA). After blocking with Blocking-One solution (Nacalai Tesque) ...
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bioRxiv - Developmental Biology 2019Quote: We prepared total protein extracts from Xenopus laevis embryos using the CelLytic Express reagent (Sigma-Aldrich) and centrifuging at full speed the extracts after 30min incubation ...
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bioRxiv - Biochemistry 2020Quote: ... we ectopically expressed the indicated proteins using the diethylaminoethyl-dextran (10 mg/mL, Cat. #D9885, Sigma)/chloroquine (2.5 mM ...
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bioRxiv - Physiology 2019Quote: ... was from BD Biosciences; citrate synthase (CS; SAB2701077) and mitochondrial fission protein 1 (FIS1; HPA017430) were from Sigma Aldrich; vitamin D receptor (D-6 ...
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bioRxiv - Microbiology 2020Quote: ... Twenty-five microliters of a 50% slurry of Protein G magnetic beads (Millipore Sigma, Cat. LSKMAGG10), pre-equilibrated in lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Neuronal soma and dendrites were identified with microtubule-associated protein 2 (MAP2, Sigma, cat no.#M4403).Neuronal axon projections and their connectivity was identified with neuron-specific class III β -tubulin (Tuj1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purified protein was concentrated using Amicon Ultra-15 centrifugal filter devices (Millipore, Billerica, MA, USA) and the buffer was replaced with storage buffer (100 mM phosphate buffer ...
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bioRxiv - Genetics 2021Quote: ... Proteins were resolved by SDS-PAGE and transferred to low fluorescence PVDF membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cell Biology 2021Quote: ... The IP samples were then incubated with 20μl of washed protein A/G–Sepharose beads (Millipore) for two hours at 4°C with constant mixing ...