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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... containing 2-fold serial dilutions of doxycycline (D9891-1G, Sigma-Aldrich Quimica S.A., Madrid, Spain) (1000-62.5 ng/mL final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) containing protease inhibitor cocktail and phosphatase inhibitor cocktails 2 and 3 (Millipore-Sigma). 15 adult Drosophila males and females of different genotypes were harvested at 10 days post-enclosure ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) containing protease inhibitor cocktail and phosphatase inhibitor cocktails 2 and 3 (Millipore-Sigma). 15 adult Drosophila males and females of different genotypes were harvested at 10 days post-enclosure ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and blocked for 5 min in PBS buffer containing 2% bovine serum albumin (BSA, Sigma). Incubation with primary antibodies in PBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% Triton-X-100) containing 2 μg/ml anti-NeuN-alexa-488 antibody (Millipore, MAB377X) and 1 μg/ml Hoechst 33342 (Molecular Probes) ...
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bioRxiv - Neuroscience 2020Quote: ... HUVECs were incubated overnight in hanging drops in EGM-2 medium containing 20% methylcellulose (Sigma) to form spheroids ...
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bioRxiv - Neuroscience 2019Quote: ... containing 2 µL of lysis buffer (0.2% Triton-X 100, 0.2% NP-40 (Sigma Aldrich), 1 U/µL RNaseOUT (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... The cells were mounted in Mowiol containing 2 mg ml-1 Hoechst 33342 (Sigma-Aldrich). Confocal fluorescence microscopy was done with a Zeiss LSM780 microscope (Carl Zeiss MicroImaging GmbH ...
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bioRxiv - Microbiology 2021Quote: ... an overlay media containing a 50:50 mix of 2% (w/v) agar (Sigma-Aldrich) and DMEM was added to each well and further incubated for 72 hours ...
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bioRxiv - Plant Biology 2019Quote: ... and sown on 0.8% (w/v) Agar (Wako) containing 1/2 MS medium (Sigma-Aldrich) and 2% (w/v ...
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bioRxiv - Physiology 2020Quote: ... were placed in a glass tank containing 2% magnesium chloride (MgCl2; Sigma-Aldrich, Gillingham, UK) dissolved in seawater ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA and 1% NP40) containing a protease inhibitor cocktail for mammalian cells (Sigma) and total protein was measured by Bradford assay 40 ...
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bioRxiv - Neuroscience 2021Quote: ... Grids washed three times in TBS and post-fixed in TBS containing 2% glutaraldehyde (Sigma) for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-mercaptoethanol) containing 10 µM SB431542 (R&D) and 3 µM CHIR99021 (Sigma), and was subsequently replaced every day ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then stained with a freshly prepared staining mix containing 2 mM CuSO4 (Sigma), 12 μM Alexa Fluor Azide 488 (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... containing 2% glucose and lacking the respective auxotrophic nutrient during selection in plates (Sigma Aldrich Uracil ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lines containing doxycycline-inducible transgenes were additionally cultured with 2 µg/mL puromycin (Sigma). Transgenes were induced with 1 µg/mL of doxycycline (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were thawed in a cryoprotectant solution containing 2% vol/vol glycerol (G5516, Sigma-Aldrich) and 20% vol/vol dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 3 hours in a blocking solution containing 2% BSA fraction V (Sigma), 0.1% TritonX-100 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... filled with the embryo medium containing 0.003% 1-phenyl 2-thiourea (PTU; P7629, Sigma-Aldrich) at 28.5°C until 72 hpf ...
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bioRxiv - Microbiology 2023Quote: ... switching larvae to E3-MB containing 0.2 nM 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) at 18-24 hours post fertilization to inhibit pigment formation ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated for 2 hours with DMEM containing 50 µM of Camostat (Sigma), Nafamostat (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... 0.05% Saponin) and resuspended in 200 μL Wash Buffer 2 containing Hoechst 34580 (Sigma, 63493) at 1 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... all samples were embedded in an agarose solution containing 2% low-melting agarose (Millipore Sigma, cat ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Physiology 2023Quote: ... A standard stock solution containing 1% 2-methyl pentanoic acid (Sigma-Aldrich St. Louis, MO) was prepared as an internal standard control for the extractions ...
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bioRxiv - Cell Biology 2023Quote: ... The agarose pad containing mounted cells was sealed with a ConA (Sigma, 2 mg/mL)-coated coverslip for immobilization of cells during imaging ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (BSA, Sigma-Aldrich) and 0.01% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Cell Biology 2020Quote: Total protein cell lysates were obtained using RIPA buffer containing protease inhibitor cocktail (11697498001, Sigma-Aldrich), phosphates inhibitors (A32957 ...
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bioRxiv - Microbiology 2021Quote: ... enabling formation of protein-containing polyacrylamide plugs upon polymerization using ammonium persulphate and TEMED (Sigma-Aldrich). Gel plugs were then diced by spinning at 14,000 xg through plastic mesh ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Immunology 2019Quote: ... We prepared protein lysates by scraping the cells in cold lysis buffer containing 1% Igepal (Sigma) and CompleteTM protease inhibitor cocktail and preclearing by centrifugation at 14,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654 ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants containing lentivirus were filtered through a low-protein binding syringe filter (0.45 μm, Millipore #SLHP033RS) before transductions ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 6 ml buffer B containing 15 mM d-desthiobiotin (Sigma Aldrich). Purification of GyrA-N-Strep and GyrB-C-Strep were conducted in the same way using an adjusted buffer Bgyr for cell disruption and purification (buffer Bgyr ...
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bioRxiv - Cancer Biology 2021Quote: ... Fractions containing the defensin protein were concentrated using a Minitan II ultrafiltration system (Millipore, Bedford, MA) with a 3-kD cutoff membrane and dialyzed against 10 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... the instrument was calibrated using a protein standard containing 10 nM β-amylase (Sigma Aldrich A8781) and 3 nM Thyroglobulin (Sigma-Aldrich 609310) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Forty micrograms of protein samples were denatured in reducing loading buffer containing β-mercaptoethanol (M3148, Sigma) and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The extracellular domain (residues 1–270) of BF2*1901 were cloned into a pET21a vector (Novagen) and transformed into Escherichia coli strain BL21 (DE3) ...