Labshake search
Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... cells were selected in a medium containing 2 μg/mL puromycin (P8833; Sigma-Aldrich), 4 μg/mL blasticidin S hydrochloride (022-18713 ...
-
bioRxiv - Neuroscience 2024Quote: ... were dissected and collected in 2 ml Eppendorf tubes containing cold DMEM (Sigma-Aldrich) and maintained on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... Peptides were resuspended in MS buffer containing 2% ACN in 1% Formic Acid (Sigma).
-
bioRxiv - Cancer Biology 2024Quote: ... 1 mM Benzamidine) containing phosphatase inhibitor cocktail 2 and 3 (Sigma, P5726 and P0044). Supernatants containing proteins were collected after centrifugation at the highest speed for 15 minutes at 4°C ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1 mL of complete DMEM containing 2 mg/mL collagenase D (Sigma-Aldrich) and 40 µg/mL DNAse I was added ...
-
bioRxiv - Cell Biology 2023Quote: ... The cryomold containing the specimens were snap-frozen on 2-methylbutane (Sigma-Aldrich #320404) pre-cooled with liquid nitrogen and stored at −80 °C ...
-
bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
-
bioRxiv - Cell Biology 2021Quote: ... Insulin secreted from the islet grafts was measured in the mouse serum three weeks post-transplant using a human insulin ELISA (Millipore, EZHI-14K).
-
bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
-
bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with PBS + 2% FBS and then resuspended in 400 μL PBS + 2% FBS containing DAPI (Millipore Sigma, D9542 ...
-
bioRxiv - Neuroscience 2023Quote: ... coverslips containing cultured DRG neurons were transferred to a new 35mm dish containing 5µM Fura-2- acetoxymethyl ester (Fura-2 AM) (Millipore Sigma 344905) in complete neurobasal media and placed in a 37°C + 0.5% CO2 incubator for 30 min ...
-
bioRxiv - Biochemistry 2022Quote: ... also contained 1-2 µg/uL α-MBPAF546 or 1-2 µg/uL mouse α-BRCA2 (Ab1, Millipore) plus 1-2 µg/µL goat α-mouse IgGAF546 (Molecular Probes).
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were eluted in HBS containing 1 mM DTT and 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
-
bioRxiv - Neuroscience 2019Quote: Frozen cortexes were homogenized in radioimmunoprecipitation assay (RIPA) buffer containing a protein inhibitor cocktail (Sigma). Samples were centrifuged at 7000 g for 5 min at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Thrombin was removed from protein-containing fractions with 40 μL p-aminobenzamidine beads (Sigma #A7155) washed twice with 400 uL 137 mM NaCl ...
-
bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... the supernatant containing solubilized SLC44A1 protein was load to anti-FLAG M2 affinity gel (Sigma), then washed by Lysis Buffer with 0.02%DDM and 0.004% CHS ...
-
bioRxiv - Biochemistry 2021Quote: ... fractions containing pure protein were concentrated using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma), dialyzed into PBS ...
-
bioRxiv - Microbiology 2020Quote: ... The eluate containing protease and cleaved protein was concentrated using Amicon Ultra 10,000 MWCO (Millipore), then passed through a Superdex-200 column (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.1% TritonX-100) containing protease and protein phosphatase inhibitor cocktails (Sigma Aldrich, Gillingham, Dorset, UK). Protein concentrations were measured with BCA protein assay and samples were boiled for 5 min at 90°C after addition of 5%β-mercaptoethanol ...
-
bioRxiv - Neuroscience 2022Quote: ... After adding 1x protein loading buffer (42526.01, SERVA) containing 5% ß-Mercaptoethanol (M6250, Sigma-Aldrich), protein samples were denatured 5 min at 95°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Total proteins of BR-EVs were extracted with RIPA buffer containing 0.5% PMSF (Sigma Aldrich). In brief ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins solubilized into serum-containing media were pulled down by using heparin-sepharose beads (Sigma). Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5) ...
-
bioRxiv - Cancer Biology 2023Quote: Equal amounts of protein or EVs were mixed with reduced (containing 5% β-mercaptoethanol, Sigma) or non-reduced 4 x Laemmli sample buffer (Bio-Rad ...
-
bioRxiv - Pathology 2019Quote: ... a Milliplex mouse cytokine magnetic kit (Millipore, France) was used according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2022Quote: ... A Milliplex Mouse Th17 Luminex kit (EMD Millipore) with analytes IL-1β ...
-
bioRxiv - Immunology 2021Quote: ... A Milliplex Mouse Th17 Luminex kit (MD MilliPore) with analytes IL-1β ...
-
bioRxiv - Biochemistry 2020Quote: ... Solubilized protein was then loaded onto 2 mL M2 affinity matrix (Sigma) previously equilibrated with wash buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
-
bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
-
bioRxiv - Immunology 2021Quote: ... Non-specific protein was blocked with 2 % normal donkey serum (Sigma Aldrich) in PBS containing 0.05 % Tween-20 and 1 % BSA ...
-
bioRxiv - Microbiology 2022Quote: The MLB1 CPNTD protein was produced in Rosetta 2 (DE3) cells (Novagen) cultured in 2×YT media with overnight expression at 18 °C induced with 0.4 mM IPTG ...
-
bioRxiv - Neuroscience 2023Quote: ... the proteins were reduced with 10mM tris(2-carboxyethyl)-phosphine (TCEP) (Sigma) for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tube containing the embryo was placed horizontally into the chamber containing 0.04% Tricaine in embryo medium (Sigma, CAS Number: 886-86-2). The tube was rotated under the microscope so that the embryo would face the objective.
-
bioRxiv - Biochemistry 2020Quote: ... the GAF domain (residues 164–313) of RcaE was cloned into plasmid pET-28 (Novagen). The construct was expressed in Escherichia coli strain BL21(DE3 ...
-
bioRxiv - Biophysics 2021Quote: ... The C2A domain (amino acids 1-129) was cloned into the pET-28a(+) vector (Novagen) between the BamHI and HindIII restriction sites ...
-
bioRxiv - Developmental Biology 2022Quote: ... Immunohistochemistry was performed using antibodies recognizing EF-Hand domain family D1 (HPA056959; RRID:AB_2683288; Sigma Aldrich), and type XV collagen (HPA017915 ...
-
bioRxiv - Biochemistry 2023Quote: ... The catalytic domains of BRSK129-358 or BRSK214-341 were sub-cloned into pET28a (Novagen) to generate N- terminal hexa-His tagged plasmid constructs for expression of BRSK1/2 catalytic domains in E ...
-
bioRxiv - Biochemistry 2020Quote: ... For ELISAs with asialofetuin (Sigma Aldrich # A4781), 5 μg/mL was immobilized ...
-
bioRxiv - Physiology 2023Quote: ... Insulin was measured by ELISA (Millipore Sigma). For glucose tolerance tests ...
-
bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...