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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... containing 10% FCS (Sigma-Aldrich, #F7524), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Sigma-Aldrich) and PenStrep (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-Fc (M4280, Sigma Aldrich) 1:100 ...
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bioRxiv - Physiology 2019Quote: Fluoxetine hydrochloride (18 mg/Kg per day; Sigma-Aldrich) was administered ad libitum in the drinking water in opaque bottles to protect it from light ...
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bioRxiv - Cell Biology 2019Quote: ... A hydrogel mixture containing 7.5-18 % acrylamide solution (Sigma) and 0.06-0.4 % bis acryl amide solution (Sigma) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The SPE columns used were DSC-18 (Supelco, Sigma). Semi-preparative HPLC separations were performed on a Shimadzu HPLC system (LC-20AP dual pump ...
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bioRxiv - Synthetic Biology 2021Quote: ... the samples were desalted by C-18 ZipTip (Millipore) and analyzed by MALDI-TOF MS 49.
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bioRxiv - Neuroscience 2020Quote: ... 18-β glycyrrhetinic acid (50 μM, Sigma-Aldrich, Germany) and TTA-P2 (3μM ...
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bioRxiv - Biochemistry 2021Quote: ... and 18% (w/v) PEG 10 K (Sigma-Aldrich) and 25% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... for 18 hours (h) or 800 μM novobiocin (Sigma) for 6 h ...
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bioRxiv - Immunology 2021Quote: ... stearylamine (18 μmoles) and cholesterol (9 μmoles) (Sigma Aldrich) was reconstituted with 300 μl of saline ...
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bioRxiv - Microbiology 2022Quote: ... using silica-gel 60 RP-18 matrix (Sigma-Aldrich). Mobile phases used were ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... Pmp70 (Sigma-Aldrich; 70-18; SAB4200181; 1:1000 WB), Rab7 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... 0.04 % p-nitrophenylphosphate (Sigma-Aldrich, CAS-333338-18-4) were added and samples were incubated until the solution turned visibly yellow ...
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bioRxiv - Biochemistry 2023Quote: ... alkylated with 18 mM iodoacetamide (IA) (Sigma, 786-228), then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... heparin (H3149-100KU, Sigma Aldrich, Mw ∼18 kg/mol); heparin-FITC (H7482 ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... ACE2 expression was primarily detected with the rabbit antibody (1:250, HPA000288, Sigma-Aldrich) and confirmed with the mouse antibody (1:5000 ...
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bioRxiv - Bioengineering 2021Quote: ... ACE2 binding was measured with an anti-FLAG HRP conjugated antibody (Sigma-Aldrich, A8592) diluted 1:1000.
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bioRxiv - Microbiology 2023Quote: The total RNA from Ace2-HEK-293T cells was isolated using TRI Reagent (Sigma) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Protein lysates were collected from regional human astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Microbiology 2023Quote: ... and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma, Cat. No. 14365C). Cells passed the MSX selection cycles were then subjected to a pilot production experiment ...
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bioRxiv - Immunology 2023Quote: ... CHO ligand anchor cells (short and long) were maintained in L-Glutamine-free DMEM (Sigma-Aldrich #D6546) supplemented with 10% dialysed FBS (dialysed three times against 10 L PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... and MG-132 (Z-Leu-Leu-Leu-CHO) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Bioworld agar (phytoagar) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... wild type (WT) hM2 mAChR expressing FlpIn CHO cells were transfected transiently using Polyethylenimine (PEI, Sigma-Aldrich) with 10 ng per well of each the G protein TRUPATH biosensors pcDNA5/FRT/TO-Gαi1-RLuc8/pcDNA3.1-β3/pcDNA3.1-Gγ9-GFP2 giving a ratio of 1:1:1 ratio with 30 ng in total ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Microbiology 2023Quote: ... mixture of BEI-EMCV strain SING-M105 or recombinant VP1 protein prepared according or BEI-EMCV with Complete Freund’s adjuvant (Sigma-Aldrich, USA) was injected intramuscularly into five BALB/c mice at the concentration of 50 μg of antigen per mouse ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...