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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The culture medium of A549-ACE2 was supplemented with 1μg/ml puromycin (Sigma). The culture medium of Caco2 and Huh-7 was supplemented with 1x non-essential amino acid ...
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bioRxiv - Microbiology 2023Quote: ... 10% SDS-PAGE gel and anti-ACE2 antibody (1:1,000, HPA000288, Sigma-Aldrich) was used ...
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bioRxiv - Bioengineering 2021Quote: ... The flask was then completely covered with aluminum foil and the solution was left to stir overnight (approximately 18 h) at RT when it was quenched with 18 mg hydroxylamine hydrochloride (Sigma-Aldrich). The solution was dialyzed with molecular weight cut-off membranes (SnakeSkin™ Dialysis Tubing ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Molecular Biology 2019Quote: ... CHO (His9) cells (JCRB Cell Bank) were grown in medium containing 10 mM L-histidinol (Sigma) to maintain human chromosome 9 ...
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bioRxiv - Neuroscience 2021Quote: CFP-DAT expressing CHO cells were fixed and immuno-labelled for DAT (1:1000, MAB369, Millipore) as above ...
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bioRxiv - Cell Biology 2020Quote: The basic production medium employed EX-CELL® Advanced™ CHO Fed-batch medium (Merck-Sigma). Bioreactors were seeded at 5×105 cells/mL at 37°C ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... CHO PS1 WT cells were incubated with 150 μM MβCD-cholesterol (cholesterol-water soluble; Sigma, #C4951) for 1 h and CHO PS1 ΔE9 cells were treated with 5 mM methyl-β-cyclodextrin (MβCD ...
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bioRxiv - Microbiology 2020Quote: CHO-HVEM cells in 24 well plates were pre-treated with 50 μM Retro-2 (Sigma) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: CHO cells were seeded on poly-L-lysine (PLL, 0.1 mg/ml, Sigma Aldrich, #P1399-100MG)-coated 13 mm glass coverslips (VWR ...
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bioRxiv - Bioengineering 2023Quote: ... CHO MPs were washed in PBS and resuspended in RIPA buffer (Millipore Sigma, Burlington, MA, USA) supplemented with protease inhibitor (200:1 buffer-to-inhibitor ratio) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant Fetuin-A (Sigma; SRP6217) was diluted in 0.9% saline vehicle solution ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant PAK1 (Millipore, 14-927) and agitated at 30°C for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant bacterial sphingomyelinase (S7651, Sigma); 2-bromopalmitate (320-76562 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse recombinant IFN-β (Millipore) diluted in DPBS with 0.1% Bovine serum albumin BSA (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Genetics 2019Quote: ... 15 U recombinant DNase1 (Sigma) and 10 U DNA polymerase I (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant DNAse I (Sigma) in MACS buffer (PBS/2mM EDTA/ 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant mouse adiponectin (Sigma, #SRP3297) and GFP protein (Sino Biological ...
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bioRxiv - Physiology 2022Quote: Lyophilised recombinant Aβ42 (#AG912, Millipore) and scrambled control peptide (ScrAβ42 ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: Recombinant Mpro (Sigma Aldrich, SAE0172) was resuspended in 10% aqueous glycerol to a concentration of 1 mg/mL ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... HA tag antibody (HA-7, Sigma-Aldrich), Myc tag antibody (A7 ...
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bioRxiv - Microbiology 2019Quote: ... and the FLAG epitope tag (F1804; Sigma), as well as anti-mouse HRP (A9044 ...
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bioRxiv - Physiology 2019Quote: ... anti-HA tag (Cat. No# H6908, Sigma), anti-Rabbit secondary (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Biochemistry 2020Quote: ... and the Flag tag (Sigma-Aldrich, F3165). ASCC2 and ASCC3 bands were quantified using Image J software ...