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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CHO-K1 cells were cultured in F-12 medium (Sigma-Aldrich, St. Louis USA) supplemented with 10% (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... The cells were then incubated with 1ug/ul primary antibody (For TRUB1 staining, TRUB1 Rabbit anti-human polyclonal, 50-172-8037, Protein tech; for PUS7 staining, PUS7 Rabbit anti-human, HPA024116, Sigma) in 1% BSA/PBS-Triton (0.1% ...
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bioRxiv - Cancer Biology 2019Quote: ... alkylated with iodoacetamide (IA) (18 mM, Sigma), then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega) ...
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bioRxiv - Bioengineering 2021Quote: ... Deionized water (18 MΩ·cm Milli-Q, Millipore) was used for rinsing samples and to prepare RGD-functionalized dendrimers’ working solutions at 2.5 10−8 ...
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bioRxiv - Microbiology 2020Quote: ... 18 g/L MgCl2•6H2O (Sigma M2393), 4.2 g/L KCl (Sigma P5405) ...
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bioRxiv - Neuroscience 2020Quote: ... L-Valine (Sigma-Aldrich, 72-18-4), L-Cysteine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 18% (w/v) Polyethylene Glycol 3350 (Sigma) at 20°C ...
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bioRxiv - Microbiology 2022Quote: ... 18-30U mL-1 pyruvate kinase (Sigma) and 27-42 U mL-1 lactate dehydrogenase (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... 18-30U mL-1 pyruvate kinase (Sigma) and 27-42 U mL-1 lactate dehydrogenase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... and alkylated with iodoacetamide (18 mM, Sigma) before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg/μL sequencing-grade trypsin (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... and 18 mM citric acid (Sigma-Aldrich) [18] and/or supplemented with 5 mM Phe (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... dissolved in deionized water (18 MΩcm, Millipore) at a concentration of 100 mM ...
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bioRxiv - Biophysics 2024Quote: ... and 18 mg/mL PMMA (200336, Sigma) in toluene were mixed ...
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bioRxiv - Molecular Biology 2024Quote: ... and 18 mg glucose oxidase (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... Expression of the NK1R-miniGs399fusion protein was induced and enhanced 18 hours after transfection with addition of 1 μg/mL doxycycline hyclate (Sigma Aldrich), 10 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... The temperature was subsequently reduced to 18°C and protein production was induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG, Sigma Aldrich) overnight ...
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bioRxiv - Microbiology 2022Quote: A549-ACE2 cells were pre-treated with 10µM of E64d (Sigma; E8640) for 1h at 37°C prior to infection ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Cell Biology 2024Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Physiology 2024Quote: ... and 120 min after intraperitoneal injection of 1g/kg glucose (glucose tolerance test) or 0.5 U/kg of recombinant human insulin (Sigma Cat.# I6634) (insulin tolerance test).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of proteins was validated by performing a Western blot analysis against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). For the EMSA assays ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Cancer Biology 2024Quote: ... HA-tag was stained using either rabbit and HA-tag from Sigma-Aldrich (catalogue no. H6908) or mouse anti-HA-Tag (6E2 ...