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Citations for Millipore Sigma :
751 - 800 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: IgG from human serum (Sigma-Aldrich) was incubated at 62°C for 30 min to induce aggregation ...
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bioRxiv - Bioengineering 2024Quote: ... or human complement C1q (Sigma-Aldrich) were immobilized on a CM5 or C1 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... 4 mg/mL human fibrinogen (Sigma), 10% Matrigel (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% human serum albumin (Sigma-Aldrich), 0.0002% heparin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Physiology 2019Quote: ... Then one of the serial sections was incubated at RT in primary antibody against a slow isoform of the myosin heavy chains and the second serial section against a fast isoform of the myosin heavy chains (M8421, 1:5000; M4276; 1:600, respectively; Sigma-Aldrich, USA) for 1 h and incubated in PBS (3 × 5 min) ...
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bioRxiv - Molecular Biology 2019Quote: ... For myosin heavy chain, the membranes were incubated with 1:500 dilution of monoclonal anti-myosin (skeletal, fast) (M4276, Sigma-Aldrich, USA) and monoclonal anti-myosin (skeletal ...
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bioRxiv - Physiology 2019Quote: ... and incubated for 2 h at room temperature with anti-myosin heavy chain I antibody (MHCI; mouse IgM, Developmental Studies Hybridoma Bank: reference #A4.480) and anti-dystrophin antibody (mouse IgG2b, Sigma Aldrich: reference #D8168) in 5% goat serum diluted in PBS (1:1 PBS dilution) ...
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bioRxiv - Microbiology 2019Quote: ... and peak identification was performed by comparison of retention times and fragmentation patterns with a standard bacterial FAME mixture containing both odd and even fatty acid chains (Supelco 47080-U; Sigma-Aldrich Ltd).
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bioRxiv - Developmental Biology 2023Quote: Inhibition of CSPG through digestion of its CS chains was done using addition of 50 mU/ml Chondroitinase ABC (ChABC; Sigma-Aldrich, USA) diluted in 0.01% BSA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Genetics 2022Quote: ... Human Erythroleukemia (HEL) and human TF-1 cells were cultured in RPMI-1640 medium (Sigma Aldrich #R8758) supplemented with 10% Fetal Bovine Serum (Sigma Aldrich #F0926 ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Neuroscience 2019Quote: ... and retina were dissected under dim red light in carbogenated (95% O2-5%CO2) Ame’s medium (Sigma, A1420). Retinas were then sliced in half and incubated at 25°C in Ame’s solution for at least 30min ...
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bioRxiv - Developmental Biology 2022Quote: Mice were either dark- or light-adapted for 12 h before ERG recording and anesthetized with 2,2,2-tribromoethanol (Sigma). After the pupils of the mice were dilated by 0.5% tropicamide ...
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bioRxiv - Cancer Biology 2022Quote: ... aliquots were protected from direct light and supplemented with 40μM EDTA and 0.5mg/ml Ascorbyl Palmitate (Sigma, PHR1455). Control 6-weeks old mice received 100μL of PBS containing 40μM EDTA and 0.5mg/ml Ascorbyl Palmitate ...
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bioRxiv - Biochemistry 2019Quote: ... SILAC-labeled heavy and light samples were then mixed and allowed to bind to streptavidin-agarose beads (Novagen) for 90 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cells were split into three different conditions light SILAC media (40 mg/mL L-Lysine K0 (Sigma, #L8662) and 84mg/mL L-Arginine R0 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and retinas were dissected under dim red light in carbogenated (95% O2-5% CO2) Ames’ medium (Sigma-Aldrich). Retinas were then sliced in half and incubated in carbogenated Ames’ medium at 26°C for at least 30 min ...
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bioRxiv - Immunology 2022Quote: ... Parasites were counted by light microscopy in thin blood smears stained with Giemsa (Sigma-Aldrich, St. Louis, MO). All animal studies were carried out in accordance with the protocol as approved by the University of Texas Medical Branch Institutional Animal Care and Use Committee.
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bioRxiv - Immunology 2023Quote: ... Parasites were counted by light microscopy in thin blood smears stained with Giemsa (Sigma-Aldrich, St. Louis, MO). Parasitemia was monitored by microscopic examination of thin blood smears from the tail ...
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Applying Super-Resolution and Tomography Concepts to Identify Receptive Field Subunits in the RetinabioRxiv - Neuroscience 2023Quote: ... The retina was then placed in a light-tight chamber that contained Ames’ medium (Sigma-Aldrich, Munich, Germany) supplemented with 6 mM D-glucose and oxygenated with a mixture of 95% O2 and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were mounted in low light in mounted using FluoroShield mounting media (Sigma-Aldrich; Product no. F6182).
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bioRxiv - Microbiology 2023Quote: ... triplicate culture samples for each light and temperature condition were vacuum filtered onto 0.2 µm PES membranes (Millipore) mounted on Nalgene filtration cups ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Neuroscience 2019Quote: ... for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain: Millipore MAB1614 1:50; Poly-GR: Millipore MABN778 1:50). The following day ...