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Citations for Millipore Sigma :
801 - 850 of 3864 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 2% HI-FBS PBS with 1 µg/mL DAPI (Sigma Aldrich, #D9542) to stain dead cells ...
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bioRxiv - Physiology 2019Quote: ... Then one of the serial sections was incubated at RT in primary antibody against a slow isoform of the myosin heavy chains and the second serial section against a fast isoform of the myosin heavy chains (M8421, 1:5000; M4276; 1:600, respectively; Sigma-Aldrich, USA) for 1 h and incubated in PBS (3 × 5 min) ...
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bioRxiv - Molecular Biology 2019Quote: ... For myosin heavy chain, the membranes were incubated with 1:500 dilution of monoclonal anti-myosin (skeletal, fast) (M4276, Sigma-Aldrich, USA) and monoclonal anti-myosin (skeletal ...
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bioRxiv - Physiology 2019Quote: ... and incubated for 2 h at room temperature with anti-myosin heavy chain I antibody (MHCI; mouse IgM, Developmental Studies Hybridoma Bank: reference #A4.480) and anti-dystrophin antibody (mouse IgG2b, Sigma Aldrich: reference #D8168) in 5% goat serum diluted in PBS (1:1 PBS dilution) ...
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bioRxiv - Microbiology 2019Quote: ... and peak identification was performed by comparison of retention times and fragmentation patterns with a standard bacterial FAME mixture containing both odd and even fatty acid chains (Supelco 47080-U; Sigma-Aldrich Ltd).
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bioRxiv - Developmental Biology 2023Quote: Inhibition of CSPG through digestion of its CS chains was done using addition of 50 mU/ml Chondroitinase ABC (ChABC; Sigma-Aldrich, USA) diluted in 0.01% BSA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Neuroscience 2019Quote: ... and retina were dissected under dim red light in carbogenated (95% O2-5%CO2) Ame’s medium (Sigma, A1420). Retinas were then sliced in half and incubated at 25°C in Ame’s solution for at least 30min ...
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bioRxiv - Developmental Biology 2022Quote: Mice were either dark- or light-adapted for 12 h before ERG recording and anesthetized with 2,2,2-tribromoethanol (Sigma). After the pupils of the mice were dilated by 0.5% tropicamide ...
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bioRxiv - Cancer Biology 2022Quote: ... aliquots were protected from direct light and supplemented with 40μM EDTA and 0.5mg/ml Ascorbyl Palmitate (Sigma, PHR1455). Control 6-weeks old mice received 100μL of PBS containing 40μM EDTA and 0.5mg/ml Ascorbyl Palmitate ...
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bioRxiv - Biochemistry 2019Quote: ... SILAC-labeled heavy and light samples were then mixed and allowed to bind to streptavidin-agarose beads (Novagen) for 90 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cells were split into three different conditions light SILAC media (40 mg/mL L-Lysine K0 (Sigma, #L8662) and 84mg/mL L-Arginine R0 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and retinas were dissected under dim red light in carbogenated (95% O2-5% CO2) Ames’ medium (Sigma-Aldrich). Retinas were then sliced in half and incubated in carbogenated Ames’ medium at 26°C for at least 30 min ...
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bioRxiv - Microbiology 2023Quote: ... triplicate culture samples for each light and temperature condition were vacuum filtered onto 0.2 µm PES membranes (Millipore) mounted on Nalgene filtration cups ...
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bioRxiv - Immunology 2022Quote: ... Parasites were counted by light microscopy in thin blood smears stained with Giemsa (Sigma-Aldrich, St. Louis, MO). All animal studies were carried out in accordance with the protocol as approved by the University of Texas Medical Branch Institutional Animal Care and Use Committee.
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bioRxiv - Immunology 2023Quote: ... Parasites were counted by light microscopy in thin blood smears stained with Giemsa (Sigma-Aldrich, St. Louis, MO). Parasitemia was monitored by microscopic examination of thin blood smears from the tail ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were mounted in low light in mounted using FluoroShield mounting media (Sigma-Aldrich; Product no. F6182).
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Applying Super-Resolution and Tomography Concepts to Identify Receptive Field Subunits in the RetinabioRxiv - Neuroscience 2023Quote: ... The retina was then placed in a light-tight chamber that contained Ames’ medium (Sigma-Aldrich, Munich, Germany) supplemented with 6 mM D-glucose and oxygenated with a mixture of 95% O2 and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... and retinas were dissected under dim red light in carbogenated (95% O2 - 5% CO2) Ames’ medium (Sigma-Aldrich). Retinas were sliced in half and incubated in carbogenated Ames’ medium at 26°C for at least 30 min ...
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bioRxiv - Biophysics 2024Quote: ... The instrument response function was collected by measuring scattered light from a colloidal suspension of Ludox (Sigma Aldrich). The lifetime decays were fit to a sum of exponentials using the following equation: where I(t ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...