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Citations for Millipore Sigma :
601 - 650 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... equipped with a Colibri 7 light source for excitation of Hoechst 33342 (cat.no. 14533, Sigma-Alrich) with LED-module 385nm ...
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bioRxiv - Biochemistry 2023Quote: ... “Light” and “heavy” media were supplemented with natural lysine and arginine (Sigma Aldrich, 0.4 mM final), and 13C- ...
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bioRxiv - Pathology 2023Quote: ... Successful crystallization was confirmed by polarized light microscopy and by SEM (Zeiss Sigma 500, Oberkochen, Germany) of crystals coated with 5 nm of Iridium (Safematic CC-010 HV) ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... a solution of 20 µg ml-1 biotinylated anti Mouse IgG antibody (#115-065-003-JIR-2ml STRATECH SCIENTIFIC LIMITED) and 20 µg ml-1 anti-Dynein Intermediate chain antibody (#MAB16818 Sigma-Aldrich). Unreacted streptavidin binding sites were then blocked using biotin-BSA (#PN29130 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4 °C with primary anti-myosin heavy chains antibody (Monoclonal Anti-Myosin skeletal, Fast, Clone My-32, Mouse Ascities Fluid, M4276, Sigma-Aldrich) diluted (1:400 ...
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bioRxiv - Biochemistry 2021Quote: ... PAR chains from a purified tetra-ADP-ribosylated H2B (1-16) peptide were subjected to treatment with Alkaline Phosphatase (Sigma-Aldrich) and Phosphodiesterase I (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
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bioRxiv - Microbiology 2022Quote: ... the medium was supplemented with a mixture of 3 branched-chain fatty acid precursors (100 μm of 2-methyl-butyrate, isobutyrate and isovalerate, Sigma-Aldrich) or straight fatty acid precursors (100 μm of methyl-butyrate ...
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bioRxiv - Plant Biology 2022Quote: Conserved regions were amplified in a standard Polymerase Chain Reaction (PCR) using genome-specific primers (Linares et al., 1998; Liu et al., 2019) synthesized commercially (Sigma-Aldrich). These probes were labelled with digoxigenin-11-dUTP ...
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bioRxiv - Genomics 2022Quote: ... Regions determined to be differential between the Illumina sequencing performed in 2017 and the PacBio sequencing performed in 2020 and 2021 were verified by polymerase chain reaction (PCR) using RedTaq (Sigma-Aldrich) according to standard protocols ...
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in biological triplicate (∼80% confluent) in HPLM plus H14 supplements and treated with 5mM of the medium-chain fatty acid OA (Sigma, #C5038) dissolved in PBS ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Physiology 2022Quote: ... To visualize traces the probes were covered with fluorescent (green light) dye (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, Sigma). LFP signals from the probes were acquired using a Digital Lynx amplifier (Neuralynx ...
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bioRxiv - Molecular Biology 2020Quote: ... Heavy/light disuccinimidyl-suberate cross-linker (DSS-H12/D12, Creative Molecules Inc.) resuspended in 100% dimethylformamide (Sigma) was added to final concentrations of 0.25 ...
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bioRxiv - Developmental Biology 2020Quote: ... Phosphopeptide standards (0.1 pmol of MS PhosphoMix 1, 2, 3 Light; Sigma-Aldrich, St. Louis, Missouri, USA) was added to suspended sample in binding/equilibration buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The tumor slides were incubated protected from light for 1’ in eosin (Sigma-Aldrich, Merck, Overijse, Belgium) and dehydrated after in 95% ethanol for 5’ ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: Light responsive endoglucanase activity was performed towards 4-nitrophenyl β-D-cellobioside model substrate (Millipore Sigma-N5759) under visible and UV light ...
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bioRxiv - Biophysics 2024Quote: ... Mineral oil (light, bioreagent, 0.84 g/mL, 14.2-17.2 cSt at 40°C) was from Sigma Aldrich.
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...