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Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 900 µl Percoll (Sigma P1644) with 100 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Neuroscience 2023Quote: ... in 2 mL HB buffer (1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, protease inhibitor (Sigma-Aldrich 04693159001), 0.3% IGEPAL-630 ...
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bioRxiv - Molecular Biology 2023Quote: ... From this 30ul was used to transduce 0.1 million HUDEP-2 cells taken in 24 well plate with 1% HEPES and 3μg polybrene (Sigma-Aldrich). Spinfection was carried out at 800G for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The organic fraction was removed from the mixture and diluted 1:2 in 100% ethanol (Sigma cat: E7023-1L). Scanning absorbance values were then taken between 230nm and 290nm on a Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: The differentiated THP-1 cell lines were seeded into 24-well plates at a density of 1 ×105 cells per well in RPMI-1640 (supplemented with 10% v/v FBS, 1% Penicillin/Streptomycin and 0.05 mM 2-mercaptoethanol) (Sigma) and incubated for 24 hours in 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden hydrogels were cut into smaller pieces and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate, 2 mM EDTA, and protease inhibitor cocktail from Sigma Aldrich). Protein content was determined using BCA assay (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... roots underwent a 2-min treatment with 1% bleach followed by immersion in boiling acid fuchsin solution (Sigma-Aldrich) for 2 to 3 min ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-acetate pH7.5, 2 mM EGTA-Tris, 1% PVP-40, 2mM DTT, 0.5×protease inhibitor cocktail [Sigma-Aldrich]). The protein concentration was determined using a Bio-Rad Bradford protein assay kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 100 µg/L in 1× TE buffer with 2 g/L heparin (Sigma-Aldrich, U.K.). Standard solutions from the corresponding RNA stock were also prepared in a 1× TE buffer with 2 g/L heparin to account for any variation in fluorescence ...
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bioRxiv - Microbiology 2024Quote: ... 20 U/mL IL-2 (AIDS Reagent Program, Germantown, MD) and 1% penicillin/streptomycin (Sigma- Aldrich, St. Louis, MO), Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences ...
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bioRxiv - Genetics 2024Quote: ... cells were washed with PBS and pellets were resuspended in 350 µl of RLT + 1% 2-Mercaptoethanol (Sigma Aldrich) and stored at −80 °C.
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bioRxiv - Molecular Biology 2024Quote: ... we grew cells in media supplemented with 1 mM or 2 mM of the drug BIX01294 G9a inhibitor (Sigma). For passaging ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 1% FBS and gluMax along with 5 μM CHIR99021 and 2 μM retinoic acid (R2625, Sigma-Aldrich) for 72 hrs ...
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bioRxiv - Evolutionary Biology 2024Quote: Tissue was minced with a scalpel into ∼1 mm3 cubes in 2 mL of digestive solution containing 0.2 mg/mL Liberase TL (05401020001, Sigma). Tissue suspensions were heated at 37°C for 15 minutes and then passed 10 times through a 16-gauge needle attached to a 3-mL syringe ...
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bioRxiv - Developmental Biology 2024Quote: ... pCAG-PiggyBac transposase or PB-pCAG-H2B-GFP (final concentration: 1-2 μg/μL) plasmid was mixed with 0.05% Fast Green (Sigma) and injected into the lateral ventricle of embryos (0.5 μL per embryo ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then perfused through the left ventricle with ice-cold 0.1 M phosphate buffer saline (PBS; pH 7.4, 4 °C) supplemented with phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2; Sigma), protease inhibitors (Complete EDTA-free Tablets ...
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bioRxiv - Neuroscience 2024Quote: ... 20-5310-05) (2 washes at 1 min each) followed by permeabilization using protease (0.34 µg/ml) (Sigma, P5380) in 1x PBS for 7.5 min ...
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bioRxiv - Biochemistry 2024Quote: Cells were collected and resuspended in lysis/IP buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 2x cOmplete protease inhibitor tablet [5056489001, SIGMA]) or IP Buffer with 0.5 mM DTT ...
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bioRxiv - Bioengineering 2024Quote: ... and chips were blocked and permeabilized for 2 hours by adding PBS containing 1% Triton X-100 (Sigma #T8787) and 3% bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μCi [γ-33P] ATP) with 1 µg substrate (purified GST-NLP7 or myelin basic protein (MBP, Sigma M1891)) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% yeast extract) and in synthetic complete (SC) medium (2% glucose, 0.67% yeast nitrogen base without amino acids (Sigma), 0.2% synthetic drop-out supplement (Sigma)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in NP40 buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40) and treated with Benzonase (Sigma) as described in 21 ...
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bioRxiv - Cell Biology 2024Quote: ... the beads with bound lysosomes were resuspended in 80 µl ice-chilled 1x extraction buffer (50 mM HEPES at pH 7.4, 40 mM NaCl, and 2 mM EDTA, 1% Triton X-100 (Sigma), 1.5 mM sodium orthovanadate (NaVO4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The cell pellets were resuspended in 2 mL lyticase buffer (1 M sorbitol, 0.1 M EDTA, 0.1% β-mercaptoethanol) plus 50 U of lyticase (Sigma) and incubated for 30 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: Cells were grown at 23°C to 6×106-1×107 cells/ml in synthetic medium before addition of cycloheximide (Sigma Aldrich, 100 mg/ml stock in 100% ethanol) to 100 µg/ml final concentration ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Plant Biology 2020Quote: ... of internode above and below the node was selected for in vitro culture on the MS-0 medium with 3 gL−1 Phytagel (Sigma Chemical Co., St. Louis) and no plant growth regulator ...
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bioRxiv - Neuroscience 2020Quote: CSF samples were prepared for NMR by adding 16 µL 3-(Trimethylsilyl)-1-propanesulfonic acid-d6 sodium salt (DSS-D6; Chenomix/Sigma-Aldrich, Oakville, ON, Canada) to 30 µL CSF (pooled from 1 – 3 animals ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Biochemistry 2021Quote: ... Fixed cells were thoroughly washed with 1XPBS and then the cells were incubated in 3% BSA with 0.1% Triton X-100 in 1XPBS (Sigma, Cat. no. 9002-93-1) for permeabilization ...
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bioRxiv - Molecular Biology 2020Quote: ... 10× lentivirus concentrates were diluted in 1 ml of Opti-MEM containing 8 × 10−3 µg/ml of polybrene (Sigma Aldrich; St. Louis, MO) and incubated overnight at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The parafilm was washed with water and blocked with 1x phosphate-buffered saline (PBS) containing 3% (w/v) BSA and then incubated in α-HA antibody (Sigma; 1:1.500 dilution) diluted in PBS buffer and 3% (w/v ...
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bioRxiv - Physiology 2022Quote: ... 3051.2) for 5 min and blocked in PBS containing 3% bovine serum albumin and 1% normal goat serum (Sigma-Aldrich, Taufkirchen, Germany, A4503 & G0023) at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... for 1-3 hours at room temperature (RT) and then permeabilized with 0.1% (v/v) Triton X-100 (Sigma-Aldrich, Cat # 9036-19-5) for 1 hour before blocking with 3% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Cell Biology 2023Quote: ... the cell lines were treated in triplicate with a top dose of 10uM in 1:3 dilutions for a total of 12 doses with paclitaxel (Sigma-Aldrich Product Number T7191), fulvestrant (Sigma-Aldrich Product Number I4409) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Cell Biology 2020Quote: Lung fragments (2 mm x 2 mm) were homogenized with TRIZOL (Sigma, Poole, UK) and RNA precipitated by overnight incubation in isopropanol at −20°C ...
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bioRxiv - Immunology 2021Quote: ... at 2 µg/mL and blocked with 2% bovine serum albumin (A8022, Sigma-Aldrich) dissolved in PBS ...