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Citations for Millipore Sigma :
7751 - 7800 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... We used standard dilutions of hen egg white lysozyme (0.5, 0.8, 1, 2, 4, 8, 10, 20, and 40 µg/ml; Sigma-Aldrich) to prepare a standard curve in each plate ...
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bioRxiv - Immunology 2020Quote: ... remaining 1% PFA was neutralized with 0.1 M glycine in PBS (1 hour) and muscles were blocked with 2% bovine serum albumin (BSA, Sigma), 1% Triton-X (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... followed by culture on gelatin for 2 weeks in hepato-medium (DMEM:F-12, supplemented with 10% FBS, 1 mg/ml insulin (Sigma), dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs were pre-treated with the following inhibitors or vehicle controls: pertussis toxin (Sigma, 1 μg/mL 2 h), SB290157 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... then placed in a 1.7 mL microcentrifuge tube with 1 mL of BY-2 media containing either 0.05% trypsin (Sigma T2601), 0.8% cellulase (Duchefa Biochemie C8001) ...
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bioRxiv - Cell Biology 2020Quote: ... MitoTEMPO ((2-(2,2,6,6-Tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride; #SML0737) and KCN (potassium cyanide; #60178) from Sigma-Aldrich; chemicals applied in Saccharomyces cerevisiae Meyen ex E.C ...
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bioRxiv - Bioengineering 2020Quote: ... according to the manufacturer’s protocol and the cDNA was diluted 1:2 in molecular biology grade water (Sigma,UK). Gene expression analysis was performed by quantitative real time PCR on a RotorGene 6000 cycler (Corbett Research ...
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bioRxiv - Biophysics 2020Quote: ... 10 μL was removed and pulsed into a tube containing 1 μL of 2 mg/mL thermolysin (Sigma Aldrich) for 1 min before quenching with 3 μL 500 mM EDTA ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Immunology 2021Quote: ... 0.05μg/mL IL-2 and activated with cell stimulation cocktail (eBioscience) for 1 hour prior to adding 10μg/ml brefeldin A (Sigma) and culturing for a further 4 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 1,000 µL transduction mix containing 1 µL of 2 µg/mL Polybrene (Sigma-Aldrich #TR-1003), recombinant lentivirus ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were finally washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min in dark ...
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bioRxiv - Plant Biology 2023Quote: ... washed three times in sterilised water and sown on half-strength Murashige and Skoog media (1/2 MS) (Sigma), 3 mM MES-KOH (pH 5.7) ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 900 µl Percoll (Sigma P1644) with 100 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Neuroscience 2023Quote: ... digestion was performed at an enzyme:protein ratio of 1:100 for 2 hours at 25°C followed by trypsin (Sigma) digestion at an enzyme:protein ratio of 1:50 overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were applied for 2 hours at room temperature and counterstained with DAPI (Sigma Aldrich D9542, 1:10000) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... weighted and incubated in 2 mL lysis solution consisting of 1% v/v Triton-X-100 (108603, Sigma-Aldrich) + 5 mM MgCl2 (M2393 ...
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bioRxiv - Immunology 2023Quote: ... lavage fluids (1/2 diluted; 100 µl) were incubated with 500 µM Gly-Pro-p-nitroanilide substrate (Sigma-Aldrich) in 200 mM Tris-HCl buffer (pH 8.3 ...
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bioRxiv - Microbiology 2023Quote: ... Intestinal tissues were then finely chopped and digested in complete HBSS-2 (HBSS, 25 mM HEPES, 1 mM sodium pyruvate) containing 0.05 mg/mL Collagenase VIII (Sigma) and 50 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... in 2 mL HB buffer (1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, protease inhibitor (Sigma-Aldrich 04693159001), 0.3% IGEPAL-630 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were treated with mitoDFX (0.03-1 µM) in the absence or presence of 2-DG (50 mM; Sigma) or oligomycin (2 µM ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl plasmid vectors (2 μg) and/or siRNA oligos (0.1 nmol) containing 0.05% fast green FCF (Sigma-Aldrich) were injected into each DRG ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The agarose pad was made by mixing 0.5 ml 2% agarose Optiprep mixture (20 mg agarose in 1 ml Optiprep (Sigma), heated to 90 °C ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA plasmids were used at 2 µg/µl and mixed with 1% fast green (Sigma-Aldrich, final concentration 0.2%). Plasmids were injected into the ventricle with a pump-controlled micropipette ...
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bioRxiv - Plant Biology 2022Quote: ... were also amplified by PCR using the primers listed in Supplemental Table 2 and cloned into pGEX4T-1 (Novagen) using BamHI/EcoRI sites to N-terminal glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was re-extracted with 450 µL methanol:water (2:1, v/v) containing 1nmol of scyllo-inositol (Sigma) as an internal standard ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipids were resuspended in chloroform: methanol (2:1 v/v) and loaded onto a silica TLC plate (Sigma 60778). For nonpolar lipid separation ...
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bioRxiv - Neuroscience 2024Quote: Lhx6-Cre;Ppargc1a+/+ and Lhx6-Cre;Ppargc1aF/F animals were injected at P1 with 600nL of custom-made AAV8-hSyn-dio-mito-EmGFP (diluted 1:2 with sterile PBS and 0.5% Fast Green, Sigma). The Vanderhaeghen lab gifted the mito-EmGFP construct ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:40 dilution for sparse cell targeting and AAV8-hSyn-dio-GFP (Addgene #50457-AAV8, 1:2 dilution with sterile PBS and 0.5% Fast Green, Sigma). The dilution factor of the DREADDs viruses (here 1:40 ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... From this 30ul was used to transduce 0.1 million HUDEP-2 cells taken in 24 well plate with 1% HEPES and 3μg polybrene (Sigma-Aldrich). Spinfection was carried out at 800G for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate, 2 mM EDTA, and protease inhibitor cocktail from Sigma Aldrich). Protein content was determined using BCA assay (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... washed 2 x 5 min in PBS and then incubated with primary antibodies against Laminin-β1γ1 (1:200; #L9393, Sigma) in 3% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: The differentiated THP-1 cell lines were seeded into 24-well plates at a density of 1 ×105 cells per well in RPMI-1640 (supplemented with 10% v/v FBS, 1% Penicillin/Streptomycin and 0.05 mM 2-mercaptoethanol) (Sigma) and incubated for 24 hours in 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The organic fraction was removed from the mixture and diluted 1:2 in 100% ethanol (Sigma cat: E7023-1L). Scanning absorbance values were then taken between 230nm and 290nm on a Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden hydrogels were cut into smaller pieces and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 150 µl of FACS buffer (PBS supplemented with 0.5 % fetal bovine serum and 2 mM EDTA) containing DAPI (1:50,000, Sigma-Aldrich) as previously described (Fischer et al ...
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bioRxiv - Bioengineering 2023Quote: ... Cell-laden were cut into 6x6 mm squares and cultured in growth medium supplemented with 0.05 mg ml-1 L-ascorbic acid-2-phosphate (Sigma) and 1 mM calcium chloride (Sigma ...