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Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 3) Beta-galactosidase (from E. Coli, Sigma-Aldrich G5635) at a concentration of ∼6 mg/mL (A280=13.79) ...
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bioRxiv - Biochemistry 2024Quote: ... 3 U/mL LDH (Sigma-Aldrich, 9001-60-9), and 294 ng CLYBL (added to launch the reaction) ...
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bioRxiv - Cell Biology 2024Quote: ... or 100μM of CK666 (Sigma-Aldrich, 442633-00-3). For inhibition of the actin cortex during osmotic shock (Figure S2H) ...
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bioRxiv - Cell Biology 2024Quote: ... 3 mM GTP and 20 μM paclitaxel (Millipore Sigma). Microtubules were centrifuged at 199,000 × g for 5 minutes to remove soluble tubulin and resuspended in BRB80 ...
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bioRxiv - Cell Biology 2024Quote: ... and 3 µM of the GSK inhibitor CHIR99021 (Sigma) (2i) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3 h or 0.2 mM hydroxyurea (Sigma-Aldrich) for 16 h ...
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bioRxiv - Cell Biology 2024Quote: ... Then 50 μL of (3-aminopropyl) triethoxysilane (Sigma-Aldrich) was added to the treated surface of the coverslip for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 µM GSK3 inhibitor CHIR 99021 (Chiron, Sigma, SML1046), 1 µM MEK inhibitor PDO35901 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977; 2% 2-heptanone in mineral oil, Sigma Aldrich product # 537683 ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Cell Biology 2020Quote: Organs from 6 weeks old wild-type C57BL/6 mice and FLAG-RIPK2 knock-in mice were lysed in IP buffer (150 mM NaCl, 50 mM Tris pH 7.5, 10% glycerol, 1% Triton X-100, 2 mM EDTA, all from SIGMA) supplemented with protease inhibitors (cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... gradients (1 for progeny population or 2 for aged population) were formed by vortexing 1.16 mL Percoll (Sigma P1644) with 42 µL 5 M NaCl and 98 µL water in 2 mL tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml Fluorobrite containing 800 μM biotin and 1000 μg/ml L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902) was added to the dish (final concentration 400 μM biotin and 500 μg/ml respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Microbiology 2021Quote: ... T84 and IPEC-1 cells were incubated in DMEM/F12 + 10% FBS + 2 µg/ml Cytochalasin D (Sigma-Aldrich) at 37 ℃ for 1 h to block actin-dependent invasion ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... RRM1 (clone ID NM_001033.2-476s1c1) and CYP1B1 (clone ID NM_000104.2-1176s1c1) were obtained in pLKO.1-Puro-CMV-tGFP from Sigma Aldrich.
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 2 mM MgCl2 and protease inhibitor cocktail [Sigma]). 10% of lysate volume was reserved to determine total protein amounts ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested in TENT buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was digested for 1 hour at 37°C in 2 mg/mL of Pronase Protease (Millipore, Temecula, CA) in growth media containing 1mM L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... The pellet was thawed by the addition of 2 mL lysis buffer: 1 mg/mL lysozyme (L6876, Millipore Sigma), 10 U/mL benzonase nuclease (E1014 ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Plant Biology 2021Quote: Clean and dry peptide samples were re-suspended in MeCN/H2O (2 %v/v) with formic acid (0. 1 %v/v) and filtered through 0.22 µm filters (Millipore) before being loaded for LC separation ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: Adult female worms were incubated with 5 μM of 5’ cy3-labeled Bma-lad-2 siRNA 1 (Sigma Aldrich) for 24 hrs to evaluate uptake of siRNA into intestinal tract epithelial cells ...
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bioRxiv - Biophysics 2020Quote: ... and adding 2 μl of catalase supernatant to 20 μl of 19.1 U⋅μL−1 of glucose oxidase (Sigma). Immediately prior to addition of trapping buffer to the chamber ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and 80% Buffer Z (0.1 M Na2HPO4, 10 mM KCl, 1 mM MgSO4, and 3.4 µl/ml 2-mercaptoethanol; all from Sigma-Aldrich). Luminescence and absorbance (410 nm ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-NL63 and SARS-CoV-2 were supplemented with 1 µg/ml TPCK-treated trypsin (Sigma-Aldrich, cat. #T1426) increasing the rate of infection ...
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bioRxiv - Neuroscience 2020Quote: ... Bacterial pellets of 20 OD600 units were lysed in 0.5 ml cold lysis buffer (50 mM Sodium-phosphate pH 8.0, 300 mM NaCl, 5% glycerol, 5 mM 2-Mercaptoethanol, 1 mM PMSF [Sigma]), RNase [0.01 mg/ml] ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Neuroscience 2020Quote: ... were each diluted in their liquid state to 133.332 Pa (1 Torr) and 266.645 Pa (2 Torr) in light mineral oil (Sigma Aldrich) which also served as the blank stimulus ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs and tumor tissue were stained with αCD8D-1-PsT and αCD8D-2-FITC in PBS supplemented with 0.5% BSA (Sigma) and EDTA (2 mM ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted in SDS extraction buffer (50 mM Tris, pH 7.8, 2 % SDS, 1 x protease inhibitor cocktail; P9599, Sigma), 2 mg/ mL NaF ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: Decreasing concentrations of recombinant DC-SIGN or rfhSP-D (2, 1, 0.5 or 0 µg 100µl/well) were coated on polystyrene microtiter plates (Sigma-Aldrich) at 4°C overnight using carbonate/bicarbonate (CBC ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and then incubated for 2 hours at room temperature in host-specific HRP-conjugated secondary antibody (1:10,000, Sigma) in TBS-T containing 5% skim milk ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Synthetic Biology 2020Quote: Growth experiments in YNB medium supplemented with yeast extract (1 g/L) and 13C-methanol (2 %; Sigma-Aldrich 277177) were carried out aerobically (sparging air ...
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bioRxiv - Cancer Biology 2020Quote: ... For each tested group 2×104 cells were added to 1 ml of 0.4% low-melt agarose solution (Sigma) in OCMI-L media and transferred to a well with a bed of growth media with 0.5% low-melt agarose ...
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bioRxiv - Cell Biology 2020Quote: ... the spheroplasts were recovered in 10 ml EMMA medium (Edinburgh minimal medium with all amino acids and nucleotides supplements) containing 0.8 M sorbitol and 0.5% (v/v) of 1 M 2-deoxyglucose (Sigma, D6134) for 30 minutes at 36°C prior to microscopy imaging.
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bioRxiv - Synthetic Biology 2021Quote: ... The material was then rehydrated by adding 1 × PBS for 5 min followed by incubation with 2% driselase (Sigma) for 60 min at 37°C in a moisture chamber to remove the cell wall ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mg/mL Dox (PanReac AppliChem #A29510025) was also added to drinking water with 1% sucrose (Sigma-Aldrich #S0389) 78,79 ...