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Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... EBs at day 5 were transferred to a new 6-well plate coated with PDL (Millipore. Catalog. MILL-A-003-E)/Laminin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Microbiology 2024Quote: ... The pooled lentivirus media was filtered through a 0.45 mm pore filter and used to transduce A549 cells in the presence of 6 μg/ml polybrene (Sigma-Aldrich, TR1003). Twenty-four hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Cell Biology 2024Quote: ... the center of the glass dish was coated with 6% Concanavalin A (Canavalia ensiformis Jack Bean, type IV Sigma C2010-G) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Systems Biology 2024Quote: ... pre-coated with Poly-L-ornithine hydrobromide (6 µg/mL in 1xPBS, Sigma-Aldrich, for 1 hour at 37°C, SigmaAldrich) followed by Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (1.2 mg/mL in 1xPBS for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Mouse head and vertebral column samples were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2,700; Sigma□Aldrich, USA) (Massalimova et al ...
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bioRxiv - Neuroscience 2024Quote: ... the glutamatergic nature of EPSCs was confirmed by a complete block of spontaneous activity with 6-cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX, 50 μM, Sigma-Aldridge, US) and D-2-amino-5-posphonopentanoic acid (APV ...
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bioRxiv - Immunology 2024Quote: ... Wells were then washed 6 times with PBS/T and developed with 100 µL of p-nitrophenyl phosphate substrate at 1 mg/mL (Sigma-Aldrich) and optical density read at 405 nm (OD405 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... 6–8-week old B6D2F1 females were hormone primed by an intraperitoneal injection of pregnant mare serum gonadotropin (PMS, EMD Millipore) followed 46 hours later by an injection of human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2024Quote: ... LC-MS grade chemicals Ammonium formate (CAS 540-69-2) and formic acid (CAS 64-18-6) were from Sigma (Germany). Authentic metabolic standards like L-alanine ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were transfected with pcDNA3.1 and pEFl vectors using GeneJuice transfection regent (Merck Millipore, #70967-3) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo, Millipore, #WBLUR0100) for chemiluminescence detection using ChemiDoc™ MP Imaging System (Bio-Rad).
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and the Arp2/3 inhibitors CK-548 and CK-636 were from Sigma (St. Louis, MO). IL-4 was from Genscript (Piscataway ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to stagetips containing 3×14G plugs of 3MTMEmporeTM SDB-RPS substrate (Sigma) as described previously (Kulak et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed in PBS and incubated in 3% bovine serum albumin (BSA) (Sigma-Aldrich) diluted in PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
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bioRxiv - Cell Biology 2020Quote: ... washed and developed with chromogenic 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma-Aldrich, B6149).
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bioRxiv - Cell Biology 2020Quote: ... organoids were rinsed twice with warm PBS and incubated with warm PBS containing 3% formaldehyde (Sigma) for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Bead solution on the column was incubated with 3 µL 50U Thrombin (EMD Millipore, Novagen®) in 250 µL wash buffer overnight at 4° C on a rotator ...
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bioRxiv - Developmental Biology 2021Quote: ... After 3 washes for 5min each with 1ml DPBS−/− (DPBS without Mg and CaCl2, Sigma, D8537), 1ml 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2021Quote: ... Freshly purified NyxB was concentrated to 21 mg/ml on 3 KDa Amicon Ultra concentrators (Millipore). SeMet-NyxB was produced in M9 minimum medium and purified as above ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... for phosphorylated histone 3 antibody staining (Chen et al., 2020) (pH3, Sigma # 05-806, 1:100), or with Lavdovsky’s fixative (3.7% formaldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... then permeabilized for 30 min in PBS containing 1.5% TritonX100 and 3% hydrogen peroxide (Sigma; #31642). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... After pre-incubation with 3% skim milk in PBS-T (PBS with 0.1% Tween20 (Sigma-Aldrich)) at RT for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was added to 3 ml anti-Flag (M2)-agarose (Sigma, St. Louis, MO, USA), and incubated at 4°C overnight ...
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bioRxiv - Physiology 2020Quote: ... and T3 (3,3’,5-triiodo-L-thyronine, > 95% HPCL, CAS number 6893-02-3, Sigma-Aldrich) were first dissolved in 0.1M NaOH and then diluted in 0.9% NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl were spotted onto plates with or without 100 μg/ml terbinafine (Sigma-Aldrich). Plates were imaged after 3–7 days of incubation at 30°C.
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bioRxiv - Molecular Biology 2020Quote: ... Following 3 x TBS-T washes the membranes were developed with Immunoblot Forte Western HRP (Millipore), according to the manufacturer’s instructions and imaged using an Amersham™ Imager 600 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168] ...
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bioRxiv - Cell Biology 2022Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used for cell viability (Sigma, Israel).
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bioRxiv - Developmental Biology 2022Quote: ... Slices were washed in PBS for 3 times and incubated in 0.3% TritonX-100 (Sigma-Aldrich,) at room temperature (RT ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing α -syn were pooled and concentrated using a 3-kDa Amicon Ultra column (Millipore). Concentrated α -syn was loaded onto a gel filtration column (Superdex 200 ...
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bioRxiv - Immunology 2019Quote: ... cells were lysed in phosphoprotein lysis buffer containing 0.25% CHAPS with phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
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bioRxiv - Pathology 2019Quote: ... at 4°C for 15 min and then subjected to blocking with 3% albumin (Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma, 1:500). All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako ...
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bioRxiv - Developmental Biology 2020Quote: ... HDLECs were incubated in a 15-mL conical tube in presence of cytodex 3 beads (Sigma) at a ratio of 400 cells per bead in EGM-2MV medium (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... All cultures were tested every 3 months for mycoplasma contamination using the LookOut kit (Sigma, MP0035).
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bioRxiv - Cell Biology 2019Quote: ... Cultures were then treated with 50 ng/mL LMB dissolved in 7:3 MeOH:H2O solution (Sigma) for 45 minutes alongside a control of the equivalent volume of MeOH before imaging.
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in permeabilization buffer (3% (w/v) bovine serum albumin (BSA, Sigma-Aldrich, Germany) with 0.5% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were diluted 1 uL into 3 uL water before monitoring peptide formation electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Neuroscience 2019Quote: ... Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP) (C2920) and 3-NP (N5636) were purchased from Sigma-Aldrich. CHX was dissolved in 95% ethanol and used at 100 µM for indicated time point ...